eFluor organic dyes spectra

Antibody conjugates offering superior optical performance

Invitrogen eBioscience eFluor organic dyes were developed specifically for labeling and detection of biomolecules. Each eFluor dye is engineered for superior optical performance in a variety of applications.

Reliable performance in multiple applications

eFluor organic dyes are traditional fluorescent dyes for use in multiple applications, including multicolor flow cytometry and immunofluorescence microscopy. To make fluorophore selection easier, all eFluor organic dyes are named after their emission wavelength and they are fully compatible with most conventional dyes.

Dual parameter scatter plot
eFluor organic dyes
DyeeFluor 450eFluor 506
Fluorophore typeOrganic dyeOrganic dye
Ex (nm)401419
Em (nm)421506
ApplicationFlow cytometryFlow cytometry
Conjugates availableeFluor 450 primary antibody conjugates

eFluor 450 secondary antibody conjugates

eFluor 506 primary antibody conjugates

Recommended bandpass filter450/50510/50
UsesComparable to Invitrogen Pacific Blue dyeUse in place of AmCyan or Horizon V500 dye
DyeeFluor 520eFluor 570PE-eFluor 610PerCP-eFluor 710

Fluorophore type

Organic dye

Organic dye

Organic dye (tandem), resonance energy transfer from PE molecule to eFluor 610 dye

Organic dye (tandem), resonance energy transfer from PerCP molecule to eFluor 710 dye

Ex (nm)

475

546

496, 565

488

Em (nm)

535

585

607

710

Application

ICC, IHC

ICC, IHC

FC

FC

Conjugates available

 

eFluor 570 primary antibody conjugates

eFluor 570 secondary antibody conjugates

PE-eFluor 610  primary antibody conjugates

PE-eFluor 610  secondary antibody conjugates

PerCP-eFluor 710 primary antibody conjugates

PerCP-eFluor 710 secondary antibody conjugates

Recommended bandpass filter

535/45

585/40

610/20

695/40

Uses

Alternative to Invitrogen Alexa Fluor 488 dye

Alternative to Invitrogen Alexa Fluor 555 dye

Alternative to Invitrogen PE-Texas Red dye

(Note: PE-eFluor 610 is brighter and requires reduced compensation from far-red channels compared to PE-Texas Red dye)

Alternative to PE-Cyanine 5.5 dye or PerCP-Cyanine 5.5

DyeeFluor 570eFluor 615
Fluorophore typeOrganic dyeOrganic dye
Ex (nm)546560
Em (nm)585645
ApplicationICC, IHCICC, IHC
Conjugates available

eFluor 570 primary antibody conjugates

eFluor 570 secondary antibody conjugates

eFluor 615 primary antibody conjugates

eFluor 615 secondary antibody conjugates

Recommended bandpass filter585/40645/75
UsesAlternative to Invitrogen Alexa Fluor 555 dyeAlternative to Invitrogen Alexa Fluor 647 dye
DyeeFluor 660APC-eFluor 780
Fluorophore typeOrganic dyeOrganic dye (tandem), resonance energy transfer from APC molecule to eFluor 780 dye
Ex (nm)633645
Em (nm)668767
ApplicationFlow cytometry, ICC, IHCFlow cytometry
Conjugates available

eFluor 660 primary antibody conjugates

eFluor 660 secondary antibody conjugates

APC-eFluor 780 primary antibody conjugates

APC-eFluor 780 secondary antibody conjugates

Recommended bandpass filter660/20780/60
UsesAlternative to APC or Invitrogen Alexa Fluor 647 dyeComparable to APC-Cyanine 7 and Invitrogen APC-Alexa Fluor 750 dye

Resources

Custom Services
Contact our eBioscience custom services group for antibody conjugation, antibody cocktails, immunoassay development, bulk reagents, and testing services.

Support

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.