Expanding options for violet laser-excitable antibody conjugates

Super Bright dyes vs. Brilliant Violet dyes for flow cytometry

  • Super Bright antibody conjugates have comparable brightness to Brilliant Violet™ conjugates 
  • Both can be utilized in flow cytometry applications, typically without a need to adjust standard workflows
  • Both dye families are violet laser–excitable polymer dyes
  • Both require use of a special buffer to reduce nonspecific dye-dye interactions when two or more polymer-dye antibody conjugates are used
     

Super Bright dye antibody conjugates selection guide

DyeSuper Bright 436Super Bright 600Super Bright 645Super Bright 702Super Bright 780
Comparable toBrilliant Violet™ 421
Brilliant Violet™ 421
Brilliant Violet™ 605
Brilliant Violet™ 605
Brilliant Violet™ 650
Brilliant Violet™ 650
Brilliant Violet™ 711
Brilliant Violet™ 711
Brilliant Violet™ 786
Brilliant Violet™ 786
Brightness
  • Similar to Brilliant Violet™ 421
  • Brighter than eFluor 450 and Pacific blue antibody conjugates
  • Similar to Brilliant Violet™ 605
  • Brighter than Pacific Orange antibody conjugates
  • Similar and sometimes superior brightness to Brilliant Violet™ 650
  • Similar to Brilliant Violet™ 711
  • Similar and sometimes superior brightness to Brilliant Violet™ 786 and Brilliant Violet™ 785
Excitation violet (405 nm)violet (405 nm)violet (405 nm)violet (405 nm)violet (405 nm)
Emission 436 nm600 nm645 nm702 nm780 nm
Suggested bandpass filter450/50610/20660/20710/50780/60
More about the dyeView the Super Bright 436 dye dashboard View the Super Bright 600 dye dashboard View the Super Bright 645 dye dashboard View the Super Bright 702 dye dashboard View the Super Bright 780 dye dashboard
Comparative dataSee dataSee dataSee dataSee dataSee data

Support

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.