Super Bright 600 Antibody Conjugates

Figure 1. Staining performance and post-fixation stability. (A) Comparison of mouse splenocytes stained with CD8a antibody conjugated to either Super Bright 600 (red, Cat. No. 63-0081-82) or Brilliant Violet™ 605 conjugate (gray), at the same antibody concentration. (B) Mouse splenocytes were stained with CD45R antibody conjugated to Super Bright 600 (Cat. No. 63-0452-82) and either left unfixed (red), or were fixed in Invitrogen eBioscience IC Fixation Buffer (Cat. No. 00-8222-49) for 30 minutes (blue), 24 hours (orange), or 3 days (green).

Figure 2. Multiplex flow cytometry experiment. Normal human peripheral blood cells were diluted in Super Bright Staining Buffer, then surface stained with the indicated reagents. Viable cells (determined using a fixable viability stain) were used for analysis to discriminate various T cell subsets.