Pierce Protein Methods | Thermo Fisher Scientific

An encyclopedia of articles about protein analysis laboratory techniques

Originally created to support Thermo Scientific Pierce products, this continually expanded and updated library is a valued resource for new and experienced researchers alike. Are you new to the protein biology laboratory and need a basic introduction to protein analysis techniques? Are you a seasoned scientist considering how to optimize an experiment involving protein expression, purification or detection? Pierce Protein Methods can help you. In addition, this educational content will assist you in choosing appropriate products from our portfolio to successfully accomplish your experiments.

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Protein Method Article Title	Protein Method Categories	Subtopics
An Overview of Cell Lysis and Protein Extraction	Cell Lysis and Fractionation	Protein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting, Protein Assays and Analysis
Traditional Methods of Cell Lysis	Cell Lysis and Fractionation	Protein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting, Protein Assays and Analysis
Detergents for Cell Lysis and Protein Extraction	Cell Lysis and Fractionation	Protein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting, Protein Assays and Analysis
Cell Lysis Solutions	Cell Lysis and Fractionation	Protein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting, Protein Assays and Analysis
Cell Fractionation and Organelle Isolation	Cell Lysis and Fractionation	Protein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting, Protein Assays and Analysis
Protease and Phosphatase Inhibitors	Cell Lysis and Fractionation	Protein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting, Protein Assays and Analysis
An Overview of Affinity Purification	Protein Purification	Protein Isolation and Purification, Protein Assays and Analysis, Protein Mass Spectrometry
Covalent Immobilization of Affinity Ligands	Protein Purification	Protein Isolation and Purification, Protein Assays and Analysis, Protein Mass Spectrometry
GST-tagged Proteins–Production and Purification	Protein Purification	Protein Isolation and Purification, Protein Assays and Analysis, Protein Mass Spectrometry
His-tagged Proteins–Production and Purification	Protein Purification	Protein Isolation and Purification, Protein Assays and Analysis, Protein Mass Spectrometry
Overview of the Immunoprecipitation (IP) Technique	Protein Purification	Protein Isolation and Purification, Protein Assays and Analysis,
An Overview of Buffer Exchange	Dialysis, Desalting, and Concentration	Protein Isolation and Purification, Protein Assays and Analysis, Protein Mass Spectrometry
Dialysis Methods for Protein Research	Dialysis, Desalting, and Concentration	Protein Isolation and Purification, Protein Assays and Analysis, Protein Mass Spectrometry
Desalting and Gel Filtration Chromatography	Dialysis, Desalting, and Concentration	Protein Isolation and Purification, Protein Assays and Analysis, Protein Mass Spectrometry
An Overview of Protein Assays	Protein Assays	Protein Assays and Analysis, Protein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting,
Chemistry of Protein Assays	Protein Assays	Protein Assays and Analysis, Protein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting,
Protein Assay Data Analysis	Protein Assays	Protein Assays and Analysis, Protein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting,
Protein-Protein Variation of Protein Assays	Protein Assays	Protein Assays and Analysis, Protein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting,
An Overview of Electrophoresis	Protein Gel Electrophoresis	Protein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting, Protein Assays and Analysis,
Protein Gel Stains	Protein Gel Electrophoresis	Protein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting
An Overview of Detection Probes	Detection Probes, ELISA, Western Blotting	Western Blotting, Protein Assays and Analysis,
Introduction to Secondary Antibodies	Detection Probes, ELISA, Western Blotting	Western Blotting, Protein Assays and Analysis, Antibodies and Proteins
Enzyme Probes	Detection Probes, ELISA, Western Blotting	Western Blotting, Protein Assays and Analysis, Antibodies and Proteins
Avidin-Biotin Interaction	Detection Probes, ELISA, Western Blotting, Protein Purification	Western Blotting, Protein Assays and Analysis, Antibodies and Proteins
Fluorescent Probes	Detection Probes, Immunohistochemistry	Western Blotting, Protein Assays and Analysis, Antibodies and Proteins
Luciferase Reporters	Reporter Gene Assays	Protein Assays and Analysis
An Overview of Western Blotting	Western Blotting	Protein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting, Protein Assays and Analysis, Antibodies and Proteins
Chemiluminescent Western Blotting	Western Blotting	Protein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting, Protein Assays and Analysis, Antibodies and Proteins
Stripping and Reprobing Western Blots	Western Blotting	, Protein Gel Electrophoresis, Western Blotting, Protein Assays and Analysis,
Fluorescent Western Blotting	Western Blotting	Protein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting, Protein Assays and Analysis, Antibodies and Proteins
Blocking Buffers for Western Blot and ELISA	Western Blotting	Protein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting, Protein Assays and Analysis
Western Blot Transfer Methods	Western Blotting	Protein Gel Electrophoresis, Western Blotting,
Chromogenic Western Blotting Substrates	Western Blotting	Protein Gel Electrophoresis, Western Blotting
Western Blot Video Tutorials	Western Blotting	Protein Gel Electrophoresis, Western Blotting
An Overview of ELISA	ELISA	Protein Assays and Analysis, Antibodies and Proteins
ELISA Development and Optimization	ELISA	Protein Assays and Analysis, Antibodies and Proteins
Spike-and-Recovery and Linearity-of-Dilution Assessment for ELISA	ELISA	Protein Assays and Analysis, Antibodies and Proteins
Factors Affecting Signal Generation in ELISA	ELISA	Protein Assays and Analysis, Antibodies and Proteins
ELISA Troubleshooting Guide	ELISA	Protein Assays and Analysis, Antibodies and Proteins
ELISA Data Analysis	ELISA	Protein Assays and Analysis, Antibodies and Proteins
An Overview of Immunohistochemistry	Immunohistochemistry	Antibodies and Proteins
Immunohistochemistry vs Immunocytochemistry	Immunohistochemistry	Antibodies and Proteins
Fixation Strategies and Formulations	Immunohistochemistry	Antibodies and Proteins
Paraffin Removal and Antigen Retrieval	Immunohistochemistry	Antibodies and Proteins
Methods to Block Endogenous Detection	Immunohistochemistry	Antibodies and Proteins
Blocking Strategies for IHC	Immunohistochemistry	Antibodies and Proteins
IHC Immunodetection	Immunohistochemistry	Antibodies and Proteins
Avidin-Biotin Complex Method for IHC Detection	Immunohistochemistry	Antibodies and Proteins
Immunofluorescence Method for IHC Detection	Immunohistochemistry	Antibodies and Proteins
IHC Counterstains	Immunohistochemistry	Antibodies and Proteins
IHC Troubleshooting Guide	Immunohistochemistry	Antibodies and Proteins
An Overview of Protein-Protein Interaction Analysis	Protein-Protein Interactions	Protein Isolation and Purification, Protein Modification and Crosslinking
Co-Immunoprecipitation (Co-IP)	Protein-Protein Interactions	Protein Isolation and Purification, Protein Modification and Crosslinking
Pull-Down Assays	Protein-Protein Interactions	Protein Isolation and Purification, Protein Modification and Crosslinking
Crosslinking Protein Interaction Analysis	Protein-Protein Interactions	Protein Modification and Crosslinking, Protein Modification and Crosslinking
Label Transfer Protein Interaction Analysis	Protein-Protein Interactions	Protein Isolation and Purification, Protein Modification and Crosslinking
Far-Western Blot Analysis	Protein-Protein Interactions	Protein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting, Protein Modification and Crosslinking
An Overview of Protein-Nucleic Acid Interactions	Protein-Nucleic Acid Interactions	Protein Modification and Crosslinking
Gel Shift Assays (EMSA)	Protein-Nucleic Acid Interactions	Protein Modification and Crosslinking
Methods for Detecting Protein-DNA Interactions	Protein-Nucleic Acid Interactions	Protein Modification and Crosslinking
Methods for Detecting Protein-RNA Interactions	Protein-Nucleic Acid Interactions	Protein Modification and Crosslinking
Chromatin IP (ChIP Assays)	Protein-Nucleic Acid Interactions	Protein Modification and Crosslinking
Methods for Labeling Nucleic Acids	Protein-Nucleic Acid Interactions	Protein Modification and Crosslinking
An Overview of Mass Spectrometry	Protein Mass Spectrometry Analysis	Protein Isolation and Purification, Protein Mass Spectrometry
Quantitative Proteomics	Protein Mass Spectrometry Analysis	Protein Isolation and Purification, Protein Mass Spectrometry
Sample Preparation for Mass Spectrometry	Protein Mass Spectrometry Analysis	Protein Isolation and Purification, Protein Mass Spectrometry
An Overview of Protein Labeling	Protein Labeling	Protein Isolation and Purification, Protein Modification and Crosslinking
Biotinylation	Protein Labeling	Protein Isolation and Purification, Protein Modification and Crosslinking
Metabolic Labeling and Chemoselective Ligation	Protein Labeling	Protein Isolation and Purification, Protein Modification and Crosslinking
An Overview of Crosslinking and Protein Modification	Protein Crosslinking	Protein Isolation and Purification, Protein Modification and Crosslinking
Chemistry of Crosslinking	Protein Crosslinking	Protein Isolation and Purification, Protein Modification and Crosslinking
Crosslinking Applications	Protein Crosslinking	Protein Isolation and Purification, Protein Modification and Crosslinking
Polyethylene Glycol (PEG) and Pegylation of Proteins	Protein Crosslinking	Protein Isolation and Purification, Protein Modification and Crosslinking
Carbodiimide Crosslinker Chemistry	Protein Crosslinking	Protein Isolation and Purification, Protein Modification and Crosslinking
Amine-Reactive Crosslinker Chemistry	Protein Crosslinking	Protein Isolation and Purification, Protein Modification and Crosslinking
Sulfhydryl-Reactive Crosslinker Chemistry	Protein Crosslinking	Protein Isolation and Purification, Protein Modification and Crosslinking
Carbonyl-Reactive Crosslinker Chemistry	Protein Crosslinking	Protein Isolation and Purification, Protein Modification and Crosslinking
Photoreactive Crosslinker Chemistry	Protein Crosslinking	Protein Isolation and Purification, Protein Modification and Crosslinking
Staudinger Ligation Reaction Chemistry	Protein Crosslinking	Protein Isolation and Purification, Protein Modification and Crosslinking
An Overview of Antibody Production and Purification	Antibody Production and Purification	Protein Expression, Antibodies and Proteins
Antibody Production (Immunogen Preparation)	Antibody Production and Purification	Protein Expression, Antibodies and Proteins
Antibody Isotyping and Characterization Methods	Antibody Production and Purification	Protein Expression, Antibodies and Proteins
Antibody Purification Methods	Antibody Production and Purification	Protein Expression, Antibodies and Proteins
Antibody IgG Binding Proteins	Antibody Production and Purification	Protein Expression, Antibodies and Proteins
Antibody Fragmentation	Antibody Production and Purification	Protein Expression, Antibodies and Proteins
Antibody Structure and Classes	Antibody Production and Purification	Protein Expression, Antibodies and Proteins
Antibody Labeling and Immobilization Sites	Antibody Production and Purification	Protein Expression, Antibodies and Proteins
An Overview of Post-Translational Modification	Post-Translational Modification	Protein Expression
Phosphorylation	Post-Translational Modification	Protein Expression
Glycosylation	Post-Translational Modification	Protein Expression
An Overview of Protein Expression Systems	Protein Expression	Protein Expression
Cell-Free Protein Expression	Protein Expression	Protein Expression
Peptide Design	Peptides	Protein Expression
Peptide Synthesis	Peptides	Protein Expression
Amino Acid Physical Properties	Peptides	Protein Expression

For Research Use Only. Not for use in diagnostic procedures.