Epitope Tag Antibodies and Related Antibodies | Thermo Fisher Scientific

Antibodies for epitope tags, fusion proteins, dyes and haptens

Image of cell fluorescently labeled with primary and secondary antibodies

Epitope tag antibodies are highly specific monoclonal and polyclonal antibodies that provide a method to localize gene products in a variety of cell types. They are used to study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance proteins when specific antibodies are not available. Our epitope tag antibodies are conjugated with the most common dyes and enzymes used by researchers. For popular targets, conjugates to biotin, HRP, and Alexa Fluor dyes are available.

We also have a wide selection of antibodies against fluorescent dyes, haptens and fusion proteins.

See all epitope tag antibodies

Quick links to antibodies for epitope tags, fusion proteins, dyes and haptens

Learn more	DYKDDDDK Tag Antibodies	GST Tag Antibodies	HA Tag Antibodies	His Tag Antibodies	Myc Tag Antibodies	TAP Tag Antibodies	V5 Tag Antibodies
See all antibodies	anti-DYKDDDDK tag antibodies	anti-GST tag antibodies	anti-HA tag antibodies	anti-His tag antibodies	anti-Myc tag antibodies	anti-TAP tag antibodies	anti-V5 tag antibodies

Learn more	Anti-GFP	Anti-mCherry
See all antibodies	anti-GFP antibodies	anti-mCherry antibodies

Learn more	Anti-FITC Antibodies
See all antibodies	anti-FITC antibodies	anti-Alexa Fluor 488 antibodies	anti-Alexa Fluor 405/Cascade Blue Antibodies	anti-BODIPY FL antibodies	anti-Lucifer Yellow antibodies	anti-Oregon Green antibodies	anti-TRITC antibodies	anti-Texas Red antibodies

Learn more	Anti-Biotin Antibodies
See all antibodies	anti-biotin antibodies	anti-dinitrophenyl-KLH antibodies	anti-nitrotyrosine antibodies

Epitope tag antibodies

Learn more	DYKDDDDK Tag Antibodies	GST Tag Antibodies	HA Tag Antibodies	His Tag Antibodies	Myc Tag Antibodies	TAP Tag Antibodies	V5 Tag Antibodies
See all antibodies	anti-DYKDDDDK tag antibodies	anti-GST tag antibodies	anti-HA tag antibodies	anti-His tag antibodies	anti-Myc tag antibodies	anti-TAP tag antibodies	anti-V5 tag antibodies

Fusion protein antibodies

Learn more	Anti-GFP	Anti-mCherry
See all antibodies	anti-GFP antibodies	anti-mCherry antibodies

Anti-dye antibodies

Learn more	Anti-FITC Antibodies
See all antibodies	anti-FITC antibodies	anti-Alexa Fluor 488 antibodies	anti-Alexa Fluor 405/Cascade Blue Antibodies	anti-BODIPY FL antibodies	anti-Lucifer Yellow antibodies	anti-Oregon Green antibodies	anti-TRITC antibodies	anti-Texas Red antibodies

Anti-hapten antibodies

Learn more	Anti-Biotin Antibodies
See all antibodies	anti-biotin antibodies	anti-dinitrophenyl-KLH antibodies	anti-nitrotyrosine antibodies

What are epitope tags?

Epitope tags are often genetically engineered into recombinant proteins during cloning, providing a method for the detection and/or purification of the expressed protein (Figure 1). They can be used to study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low-abundance proteins when specific antibodies are not available. Epitope tag antibodies are commonly used in structure/function studies where modified constructs are compared against native proteins.

Schematic of tag incorporation into protein 3 step process

Figure 1. Basic method for incorporating epitope tags into recombinant proteins.

Different types of epitope tags

What are peptide tags?	What are protein tags?	What are fluorescent tags?

Short amino acid sequences. Some epitope tags, such as myc, are often used in tandem to increase their specific detection, or in combination with another tag.	Large-sized proteins when tagged with a protein of interest result in fusion protein.	Large-sized proteins when tagged with a protein of interest result in fusion protein and provide a fluorescent label to the protein of interest.
Examples: Hemagglutinin (HA), His, myc, V5, FLAG tag (DYKDDDDK)	Examples: GST	Examples: GFP, RFP, mCherry
Uses: Enable easy affinity purification of expressed proteins Enable localization and expression of proteins Used in pull-down immunoprecipitation (IP) experiments	Uses: Can aid in protein expression— enhances the solubility of expressed proteins GST can be easily purified using glutathione agarose	Uses: Monitor protein–protein interactions by Can aid in protein expression— enhances the solubility of expressed proteins GST can be easily purified using glutathione agarose
Applications: Western blot, ELISA, chromatin IP (ChIP), immunocytochemistry (ICC), and immunohistochemistry (IHC)	Applications: IP, western blot, ELISA, ChIP, ICC, and IHC	Applications: Western blot, ELISA, IHC, and fluorescence measurement
Detection: By antibodies either directly conjugated to epitope tags or detected with secondary antibodies	Detection: By antibodies either directly conjugated to epitope tags or detected with secondary antibodies	Detection: By innate fluorescence of the epitope tags or by antibodies when other detection methods are needed

Name	Type of tag	Sequence/size	Description
HA	Peptide tag	YPYDVPDYA	A highly immunoreactive epitope and can be used to purify tagged target proteins; can be used for co-IP studies and western blots.
His	Peptide tag	6X-His HHHHHH tag	Widely used tag for purification of target proteins that can be used with almost all expression systems.
FLAG	Peptide tag	DYKDDDDK	The high hydrophilicity and small size of the FLAG tag tend to interfere less with protein expression, proteolytic maturation, antigenicity, and function.
Myc	Peptide tag	EQKLISEEDL	A reliable method for the detection and purification of tagged target proteins without a protein-specific antibody or probe.
V5	Peptide tag	GKPIPNPLLGLDST	Widely used in affinity purification in combination with His-tag.
GST	Protein tag	27 kDa	Highly soluble protein, resulting in greater expression and solubility of recombinant proteins with the GST tag, and can be especially helpful when expressing proteins in bacteria; the GST protein also has a strong affinity for its substrate, glutathione.
GFP	Fluorescent protein tag	27 kDa	A versatile marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression. GFP is also a powerful research tool for assessing gene expression and subcellular protein distribution in fixed or living cells.
mCherry	Fluorescent protein tag	28 kDa	Due to its improved brightness, superior photostability, and extremely rapid maturation rate, the mCherry monomeric red protein is one of the fluorescent proteins of choice for monitoring physiological processes and detecting transgenic expression.