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Gibco GlycanTune Total Feeds
Precision meets predictability
Achieve consistent protein quality by dialing in your specific glycan profile with Gibco GlycanTune Total Feeds. Simplify your glycan targeting with the ability to shift glycan profiles from heavily G0F to primarily G1F and G2F glycans. Take the guesswork out of your processes.
Gibco GlycanTune Total Feeds are chemically defined, animal origin–free supplements that are pH-neutral and easy-to-use, giving you more control and consistency to obtain your ideal glycan profiles.
Control and consistency for your processes
Enhance your capabilities to achieve your desired glycan profiles with GlycanTune Total Feeds.
- Replace your normal feeds with GlycanTune Total Feeds at the time of glycan shifting and obtain a predictable, linear glycan response.
- Improve protein quality by shifting glycan expression to mostly G1 and G2, with as much as 45% reduction in G0, and without significant loss in performance.
- Gain ~20% more bioreactor utilization with less product dilution, more space for an additional feeding and/or more working volume to start your run.
- Store hydrated product at room temperature for up to 30 days, use fewer bioreactor connections, and reduce the chances of contamination.
- Simple reconstitution with just water results in fewer parts, fewer reconstitution steps, and reduced opportunities for operator error.
Maximize your bioreactor space
By utilizing Gibco EfficientFeed + supplements, you can achieve up to 20% in bioreactor space utilization. The flexibility to super-concentrate Gibco EfficientFeed+ supplements allows for a reduction in dilution, or maximization of either working volume or total amount of fed nutrients to help you conserve bioreactor space.
Stop the guesswork. GlycanTune Total Feeds work in conjunction with Gibco EfficientFeed Supplements or with your own feeds.
Using a simple experiment targeting the right profile becomes easy.
Figure 1: The timing of transition from Gibco EfficientFeed C+ supplement (EFC+) to GlycanTune C+ Total Feed (GTC+) makes it possible to target specific glycosylation profiles. Modulate G0F from 75% down to 32% while increasing G1F (1 and 2) and increasing G2F.
Protein quality has been seen to improve by reducing G0 expression by up to 45%.
Figure 2: Protein quality improved overall as shown by an increase in G1F and G2F glycoforms compared to the control. Transitioning to GlycanTune C+ earlier results in lowering G0F glycoforms by up to 40%, while still maintaining growth and productivity.
Just add water; reconstitution requires fewer steps; auto-adjusts pH and osmolality.
For use with EfficientFeed Supplements, download the selection guide to best match with your CHO cells.
For use with your own feeds, download the user manual for your best selections.
Or contact one of our Gibco BioProduction Service scientists for a no hassle consultation.
GlycanTune Total Feeds are available in A+, B+, or C+ formulations.
Available starter kits include:
- GlycanTune A+, B+, or C+ Total Feeds
- Corresponding EfficientFeed A+, B+, or C+ Supplements
Flashdrive with user manual
Consider improving your glycan analysis with our novel, highly sensitive fluorescent dy-based system with the Applied Biosystem GlycanAssure Glycan Analysis and Quantitation System.
Glycosylation is a critical quality parameter that is examined closely in regulatory filings. Companies must show a range of control over the pattern they get from their bioreactor runs for consistency of process and performance of the protein being produced. In the case of companies making a biosimilar, they must match an innovator molecule glycosylation pattern closely. For others, it may be a matter of showing consistency and perhaps targeting a profile that may make pharmacodynamics parameters of the molecule more desirable.
Until recent years, control was dictated by the cells used and clones generated, necessitating screening of many clones and/or time-consuming development of cells that had been engineered to express specific glycoforms. More recently, there are published components known to influence glycosylation attributes in cell culture that customers have used to control the glycosylation patterns achieved in some cases. Additionally, there are process parameters that can influence other aspects of process quality that are being studied.
GlycanTune Total Feeds provide components needed to move glycan patterns from mostly G0F to G1F and G2F. This is based on the inclusion timing during the feeding process. The products also contain standard feeding supplement components so that they can be used as a substitute for other feeding when the time is right to gain the glycosylation shift desired.
These Total Feeds can be switched to during a feeding schedule at the point in time that provides the desired shift in glycan profile. That can be determined in a simple experiment that is outlined in a manual provided with testing kits that can help a customer determine the best timing to introduce GlycanTune Total Feeds as part of a daily feeding schedule or less frequent feeding schedule.
GlycanTune Total Feeds were developed for use up to production level scales in the thousands of liters of bioreactor process.
GlycanTune Total Feeds are complete feeding supplements patterned after EfficientFeed A+, B+ and C+ supplements that can be super concentrated and reconstituted by just adding water (no pH adjustments required) to deliver up to 200g/L amounts of nutrients cells may need to actually increase titers. Super concentrating to 2X or 3X will allow for the possibility of adding more feed into the bioreactor without diluting the final product and while utilizing available bioreactor space.
Stop the guesswork. GlycanTune Total Feeds work in conjunction with Gibco EfficientFeed Supplements or with your own feeds.
Using a simple experiment targeting the right profile becomes easy.
Figure 1: The timing of transition from Gibco EfficientFeed C+ supplement (EFC+) to GlycanTune C+ Total Feed (GTC+) makes it possible to target specific glycosylation profiles. Modulate G0F from 75% down to 32% while increasing G1F (1 and 2) and increasing G2F.
Protein quality has been seen to improve by reducing G0 expression by up to 45%.
Figure 2: Protein quality improved overall as shown by an increase in G1F and G2F glycoforms compared to the control. Transitioning to GlycanTune C+ earlier results in lowering G0F glycoforms by up to 40%, while still maintaining growth and productivity.
Just add water; reconstitution requires fewer steps; auto-adjusts pH and osmolality.
For use with EfficientFeed Supplements, download the selection guide to best match with your CHO cells.
For use with your own feeds, download the user manual for your best selections.
Or contact one of our Gibco BioProduction Service scientists for a no hassle consultation.
GlycanTune Total Feeds are available in A+, B+, or C+ formulations.
Available starter kits include:
- GlycanTune A+, B+, or C+ Total Feeds
- Corresponding EfficientFeed A+, B+, or C+ Supplements
Flashdrive with user manual
Consider improving your glycan analysis with our novel, highly sensitive fluorescent dy-based system with the Applied Biosystem GlycanAssure Glycan Analysis and Quantitation System.
Glycosylation is a critical quality parameter that is examined closely in regulatory filings. Companies must show a range of control over the pattern they get from their bioreactor runs for consistency of process and performance of the protein being produced. In the case of companies making a biosimilar, they must match an innovator molecule glycosylation pattern closely. For others, it may be a matter of showing consistency and perhaps targeting a profile that may make pharmacodynamics parameters of the molecule more desirable.
Until recent years, control was dictated by the cells used and clones generated, necessitating screening of many clones and/or time-consuming development of cells that had been engineered to express specific glycoforms. More recently, there are published components known to influence glycosylation attributes in cell culture that customers have used to control the glycosylation patterns achieved in some cases. Additionally, there are process parameters that can influence other aspects of process quality that are being studied.
GlycanTune Total Feeds provide components needed to move glycan patterns from mostly G0F to G1F and G2F. This is based on the inclusion timing during the feeding process. The products also contain standard feeding supplement components so that they can be used as a substitute for other feeding when the time is right to gain the glycosylation shift desired.
These Total Feeds can be switched to during a feeding schedule at the point in time that provides the desired shift in glycan profile. That can be determined in a simple experiment that is outlined in a manual provided with testing kits that can help a customer determine the best timing to introduce GlycanTune Total Feeds as part of a daily feeding schedule or less frequent feeding schedule.
GlycanTune Total Feeds were developed for use up to production level scales in the thousands of liters of bioreactor process.
GlycanTune Total Feeds are complete feeding supplements patterned after EfficientFeed A+, B+ and C+ supplements that can be super concentrated and reconstituted by just adding water (no pH adjustments required) to deliver up to 200g/L amounts of nutrients cells may need to actually increase titers. Super concentrating to 2X or 3X will allow for the possibility of adding more feed into the bioreactor without diluting the final product and while utilizing available bioreactor space.
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GlycanTune products
Related products
- Pair with GlycanTune Total Feeds to streamline your workflow
- Process 96 samples in parallel
- Complete analysis on the Applied Biosystems 3500cL CE System in less than 9 hours
Consider Gibco EfficientFeed A+ AGT Supplement when:
- You wish to explore simpler reconstitution and super-concentration benefits of new technology for delivery of key components
- You are currently using Gibco CD OptiCHO Medium as your base cell growth medium
Related products and services
Gibco Dynamis Medium
Gibco Dynamis Medium provides the power to achieve higher titers, faster process development, and seamless scale-up.
Applied Biosystems GlycanAssure Glycan Analysis and Quantitation System
The first glycan analysis platform that combines both high throughput and high data quality, and offers an integrated glycan analysis platform that helps save labor, time, and cost of analysis.
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