Graph of NovaFluor Blue 760 dye excitation and emission
3488780/60490764(in buffer) 4flow cytometry

NovaFluor dyes, including NovaFluor Red 760 dye, are built using Phiton technology and are compatible with both spectral flow cytometry and traditional flow cytometry. NovaFluor dyes exhibit narrow emission spectra and minimal cross-laser excitation, which helps reduce spectral spillover for better marker resolution. In addition, their unique spectral signatures can provide the opportunity to detect additional markers in flow cytometry panels by opening up previously unusable channels.

NovaFluor Blue 760 dye has a unique emission spectrum when excited by the 488 nm laser, which fits in a spectral space that is currently not accessible by other spectral dyes. Adding spectrally unique dyes may allow for building out larger panels with minimal loss of resolution. NovaFluor Blue 760 dye should generally be paired with moderately to highly expressed antigens in multicolor panels. The macromolecule-based NovaFluor Blue 760 dye produces highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C.

Benefits of NovaFluor Blue 760 dye

  • Narrow emission spectrum and minimal cross-laser excitation for better resolution
  • Should be paired with moderately expressed antigens to minimize spillover
  • Spectrally unique and can be used to fill unused detectors in conventional flow cytometry
  • Produces stable fluorescence and is compatible with all buffers tested

Use NovaFluor dyes with CellBlox Plus Blocking Buffer to reduce background and to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.

NovaFluor Blue 760 primary antibodies

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Experimental data using NovaFluor Blue 760 dye
Graph of fluorescence intensity vs channel for NovaFluor Blue 760 dye

Figure 1. Spectral signature of NovaFluor Blue 760 dye. Normal human peripheral blood cells stained with anti-CD4 antibodies (clone SK3) conjugated to NovaFluor Blue 760 dye were used for analysis. Data was acquired on a 5-laser Cytek Aurora system.

2-panel scatter plots of mouse splenocytes, unstained vs CD45R-stained and CD5-stained vs CD45R-stained

Figure 2. NovaFluor Blue 760 conjugated antibody staining performance with mouse splenocytes.  C57BL/6 mouse splenocytes were stained with CD45R Monoclonal Antibody, eFluor 450 only (left) or along with CD5 Monoclonal Antibody, NovaFluor Blue 760 (right). Viable cells were used for analysis, as determined by LIVE/DEAD Fixable Blue Dead Cell Stain Kit (Cat. No. L34962).

2-panel scatter plots of human monocytes, unstained vs CD14-stained and CD11b-stained vs CD14-stained

Figure 3. NovaFluor Blue 760 conjugated antibody staining performance with human PBMCs. Normal human peripheral blood cells were stained with CD14 Monoclonal Antibody, APC-eFluor 450 only (left) or with CD11b Monoclonal Antibody, NovaFluor Blue 760 (right). Cells in the monocyte gate were used for analysis.

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