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NovaFluor dyes, including NovaFluor Yellow 590 dye, are built using Phiton technology and are compatible with both spectral flow cytometry as well as traditional flow cytometry. NovaFluor dyes exhibit narrow emission spectra and minimal cross-laser excitation, reducing spectral spillover for better marker resolution. In addition, their unique spectral signatures can provide the opportunity to detect additional markers in flow cytometry panels by opening up previously unusable channels.
NovaFluor Yellow 590 dye has a unique emission spectrum, which fits in between PE and PE-tandems such as PE-Dazzle 594, potentially allowing the detection of an additional marker in a complex multicolour panel. However due to spectral spillover by PE and PE-tandems, an optimal panel design would replace PE and PE tandems with NovaFluor Yellow 570 and NovaFluor Yellow 610 to reduce spillover into NovaFluor Yellow 590. NovaFluor Yellow 590 dye should generally be paired with medium to highly expressed antigens to minimize spectral spillover in complex multicolor panels. The macromolecule-based NovaFluor Yellow 590 dye produces highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C.
Use NovaFluor dyes with CellBlox Monocyte and Macrophage Blocking Buffer to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.
We offer NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as, NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.
Figure 1. Absorption and fluorescence emission spectra of NovaFluor Yellow 590 dye.
Figure 3. Staining of normal human peripheral blood cells with side scatter and unstained (left) or CD4 Monoclonal Antibody, NovaFluor Yellow 590 (Cat. No. H001T03Y02) (right). Data was acquired in the YG2 channel on a 5-laser Cytek Aurora system, and singlet cells were used for analysis.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and is used under license.
For Research Use Only. Not for use in diagnostic procedures.