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Precast Agarose Gels |
Fast, convenient DNA electrophoresis
E-Gel precast gels are bufferless agarose gels with electrodes embedded in the agarose matrix. Each precast agarose gel contains an ion generating system, a pH balancing system, and DNA stain packaged inside a transparent plastic cassette. It also contains two ion exchange matrices (IEMs) that are in contact with the gel and electrodes. The IEMs supply a continuous flow of ions throughout the precast gel resulting in a sustained electric field required for running the gel.
Features of E-Gel precast agarose gels
- Ready-to-use—no gel preparation or additional buffers required
- Safer analysis—choose E-Gels containing SYBR Safe DNA stain, a safer alternative to ethidium bromide
- High performance—detect 2–3 ng of sample DNA in less than 30 minutes
- Wide variety of applications—large selection of agarose percentages, well formats, and throughput capacities to suit your applications
E-Gel precast agarose gels with SYBR safe gel stain
Experience safer analysis without gels to pour, buffers to make, or chemical staining. Invitrogen E-Gel Precast Agarose Gels with SYBR Safe Gel Stain are designed for safer, more convenient DNA electrophoresis. Use the selection guide below to find precast gels containing SYBR safe gel stain by application.
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E-Gel precast agarose gel selection guide
E-Gel precast agarose gels are available in a variety of agarose percentages, well formats, and throughput capacities to suit many applications. Use the guide provided below to choose which precast agarose gel works best for the application in consideration.
E-Gel precast gels for low throughput DNA electrophoresis
For general purpose DNA electrophoresis or high sensitivity and fast separation; there is an E-Gel precast gel suited for many low throughput agarose gel electrophoresis applications.
| Goal | General purpose | High sensitivity and faster separation | ||
|---|---|---|---|---|
| Precast gel types | E-Gel | E-Gel EX | ||
| Stain | SYBR Safe DNA stain | SYBR Gold II DNA stain | ||
| Run time | 13–40 min | 5–20 min | ||
| Number of wells | Single row of 11 wells | 22 wells in a double comb design | Single row of 11 wells | 22 wells in a double comb design |
| Agarose % | ||||
| Resolution | 10 bp–5 kb | 50 bp–5 kb | 10 bp–5 kb | 50 bp–5 kb |
| Sample volume | 20 µL | 20 µL | 20 µL | 20 µL |
E-Gel 48- and 96-well precast agarose gels for high-throughput DNA electrophoresis
E-Gel 48- and 96-well agarose gels are ideal for high-throughput electrophoresis and analysis of PCR products, plasmid preparations, and restriction digests. E-Gel 48 gels include two rows each containing 24 samples and two molecular weight marker wells with a 3.2 cm length. E-Gel 96 gels contain 96 sample lanes and eight marker lanes, staggered to provide a 1.6 cm run length with resolution between 100 bp and 10 kb. This staggered-well format is compatible with most robotic loading systems.
| Goal | High throughput | |
|---|---|---|
| Precast gel type | E-Gel 48-well gels | E-Gel 96-well gels |
| Number of wells | 48 sample wells and 4 marker wells | 96 sample wells and 8 marker wells |
| Stain | SYBR Safe DNA stain or Ethidium bromide | SYBR Safe DNA stain or Ethidium bromide |
| Agarose % | ||
| Resolution | 10 bp–10 kb | 100 bp–10 kb |
| Run time | 17–25 min | 12–17 min |
| Sample volume | 15 µL | 20 µL |
E-Gel precast gels for cloning
Using E-Gel CloneWell II agarose gels with the E-Gel Power Snap Electrophoresis System eliminates UV damage and improves cloning efficiency. E-Gel CloneWell II agarose gels are double-comb gels designed for the sample to be loaded into the top well, electrophoresed, and bands can then be retrieved once they migrate into the collection well. No additional purification is required.
| Goal | High efficiency cloning |
|---|---|
| Precast gel type | E-Gel CloneWell II |
| Number of wells | Two rows of 7 wells (for 7 samples) |
| Stain | SYBR Safe DNA stain |
| Agarose % | 0.8% |
| Resolution | 100 bp–6 kb |
| Run time | 13–28 min |
| Sample volume | 20–25 µL |
E-Gel precast gels for NGS library preparation
For NGS library prep, the ability to recover a purified DNA fragment is critically important. Choose the E-Gel precast agarose gel that suits the throughput and precision needs of your NGS sample prep workflow.
| Goals | Precise DNA fragment library size selection | High range fragment library separation |
|---|---|---|
| Precast gel types | E-Gel SizeSelect II | E-Gel NGS |
| Number of wells | Two rows of 7 wells (for 7 samples) | Single row of 11 wells |
| Stain | SYBR Gold stain | SYBR Safe DNA stain |
| Agarose % | 2.0% | 0.8% |
| Resolution | 50 bp–2 kb | 800 bp–10 kb |
| Run time | 8–20 min | 26–32 min |
| Sample volume | 20–25 µL | 20 µL |
E-Gel precast gels for low throughput DNA electrophoresis
For general purpose DNA electrophoresis or high sensitivity and fast separation; there is an E-Gel precast gel suited for many low throughput agarose gel electrophoresis applications.
| Goal | General purpose | High sensitivity and faster separation | ||
|---|---|---|---|---|
| Precast gel types | E-Gel | E-Gel EX | ||
| Stain | SYBR Safe DNA stain | SYBR Gold II DNA stain | ||
| Run time | 13–40 min | 5–20 min | ||
| Number of wells | Single row of 11 wells | 22 wells in a double comb design | Single row of 11 wells | 22 wells in a double comb design |
| Agarose % | ||||
| Resolution | 10 bp–5 kb | 50 bp–5 kb | 10 bp–5 kb | 50 bp–5 kb |
| Sample volume | 20 µL | 20 µL | 20 µL | 20 µL |
E-Gel 48- and 96-well precast agarose gels for high-throughput DNA electrophoresis
E-Gel 48- and 96-well agarose gels are ideal for high-throughput electrophoresis and analysis of PCR products, plasmid preparations, and restriction digests. E-Gel 48 gels include two rows each containing 24 samples and two molecular weight marker wells with a 3.2 cm length. E-Gel 96 gels contain 96 sample lanes and eight marker lanes, staggered to provide a 1.6 cm run length with resolution between 100 bp and 10 kb. This staggered-well format is compatible with most robotic loading systems.
| Goal | High throughput | |
|---|---|---|
| Precast gel type | E-Gel 48-well gels | E-Gel 96-well gels |
| Number of wells | 48 sample wells and 4 marker wells | 96 sample wells and 8 marker wells |
| Stain | SYBR Safe DNA stain or Ethidium bromide | SYBR Safe DNA stain or Ethidium bromide |
| Agarose % | ||
| Resolution | 10 bp–10 kb | 100 bp–10 kb |
| Run time | 17–25 min | 12–17 min |
| Sample volume | 15 µL | 20 µL |
E-Gel precast gels for cloning
Using E-Gel CloneWell II agarose gels with the E-Gel Power Snap Electrophoresis System eliminates UV damage and improves cloning efficiency. E-Gel CloneWell II agarose gels are double-comb gels designed for the sample to be loaded into the top well, electrophoresed, and bands can then be retrieved once they migrate into the collection well. No additional purification is required.
| Goal | High efficiency cloning |
|---|---|
| Precast gel type | E-Gel CloneWell II |
| Number of wells | Two rows of 7 wells (for 7 samples) |
| Stain | SYBR Safe DNA stain |
| Agarose % | 0.8% |
| Resolution | 100 bp–6 kb |
| Run time | 13–28 min |
| Sample volume | 20–25 µL |
E-Gel precast gels for NGS library preparation
For NGS library prep, the ability to recover a purified DNA fragment is critically important. Choose the E-Gel precast agarose gel that suits the throughput and precision needs of your NGS sample prep workflow.
| Goals | Precise DNA fragment library size selection | High range fragment library separation |
|---|---|---|
| Precast gel types | E-Gel SizeSelect II | E-Gel NGS |
| Number of wells | Two rows of 7 wells (for 7 samples) | Single row of 11 wells |
| Stain | SYBR Gold stain | SYBR Safe DNA stain |
| Agarose % | 2.0% | 0.8% |
| Resolution | 50 bp–2 kb | 800 bp–10 kb |
| Run time | 8–20 min | 26–32 min |
| Sample volume | 20–25 µL | 20 µL |
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