SYBR Green Real-Time PCR Master Mixes

SYBR Green real-time PCR master mix

As the pioneers of SYBR Green dye quantification for qPCR, we have evolved our SYBR Green master mix formulations over many years into highly consistent and reliable master mixes that deliver exceptional sensitivity and specificity.


Our latest formulation, PowerTrack SYBR Green Master Mix, has been optimized to increase efficiency by reducing pipetting errors via an optional sample tracking dye and to minimize primer optimization by working with a broad primer Tₘ range.

PowerTrack SYBR Green Master Mix

 
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Chemistry

2-step SYBR green-based

Target input

cDNA

Concentration

2X

Multiplexing and passive reference dye

With ROX: 1 target

Cycling mode/run time

Fast: ~30 min

Standard: ~60 min

Carryover contamination control

With UNG

Reverse transcriptase

Purchase SuperScript IV Vilo Master Mix separately and use before qPCR

Assay recommendations

Sequence detection primers

 

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Note: when you need up to 72 hours of benchtop stability, choose PowerUp SYBR Green Master Mix in combination with High-Capacity RNA-to-cDNA Kit Reverse Transcriptase.

For Research Use Only. Not for use in diagnostic procedures.