TaqMan real-time PCR master mixes

Applied Biosystems TaqMan master mixes have been optimized for use across a wide variety of research and discovery-stage biopharmaceutical applications. Choosing the right master mix depends on your target (RNA or DNA), application, test format, and multiplexing needs. For most qPCR research applications, we recommend using either TaqMan Fast Advanced Master Mix or TaqMan Fast Virus 1-Step Master Mixes

Which TaqMan Master Mix is right for you?

 


TaqMan Fast Advanced Master Mix


TaqMan Fast Virus 1-Step Master Mixes

 

Check price or order now

Check price or order now

Key applications

  • Gene expression analysis (2-step)
  • DNA detection
  • miRNA analysis
  • Gene expression analysis (1-step)
  • RNA virus detection

 

Target

DNA, including cDNA

RNA

Concentration

2X

4X

Multiplexing and passive reference dye

With ROX:  up to 2 targets

Available in 2 versions:

With ROX: up to 3 targets

No ROX: 3 or more targets 

Cycling mode/run time

Fast:  ~40 min

Standard: ~50 min

Fast: ~60 min

Standard: ~90 min

Carryover contamination control

Available in 2 versions:

With UNG

No UNG

No UNG

Reverse transcriptase

Purchase SuperScript IV VILO Master Mix separately and use before qPCR

Thermostable MMLV RT included in master mix

Assay recommendations

TaqMan Gene Expression assays

TaqMan Gene Expression assays

 

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