Protein Dialysis, Desalting, Concentrators, and Small Molecule Removal

Salts and detergents used in protein sample preparation can adversely affect protein function and stability, interfering with downstream analyses. We offer a variety of resins and specialty devices in multiple formats for the efficient removal of salts and small molecule contaminants from protein samples. These products exhibit efficient and consistent performance and high sample recovery. In many cases, downstream analyses may also require that proteins and other biomolecules be at a higher concentration. To this end, we offer ultrafiltration protein concentrator devices that accommodate a wide range of sample volumes, remove excess liquids and buffers, and even exchange buffers. For removal of small molecules such as unconjugated fluorescent dyes, biotin, excess crosslinkers, and other reaction by-products, Pierce Dye and Biotin Removal Spin Columns can be used to achieve exceptional results.

Pierce Dye and Biotin Removal columns enable fast and efficient removal of non-reacted fluorescent dyes, biotin, reducing agents, and crosslinkers from samples containing proteins of interest that are larger than 7 kDa.

Simplify sample concentration, cleanup, and buffer exchange with Thermo Scientific Pierce Concentrators, which are available in five distinct molecular weight cutoffs (MWCOs) for volumes ranging between 100 μL–6 L.

Slide-A-Lyzer devices, cassettes, and flasks in a variety of formats and MWCOs, Pierce 96-well microdialysis plates, & SnakeSkin Dialysis Tubing.

Detergent removal resins in two formulations—optimized for high- or low-peptide concentrations—available in spin columns and 96-well spin filter plates.

Quickly remove salts and small molecules from protein solutions using Thermo Scientific Zeba Spin Columns, plates, and cartridges.

The Thermo Scientific Pierce RED system with easy-to-use inserts or preloaded plates for automation-compatible plasma protein binding assays.

Mini- and microcentrifuges to help accelerate your protein preparation protocols with RCFs up to 21,000 x g in compact, safe, easy-to-use instruments.

Designed for solid performance and consistent results, our benchtop centrifuges deliver efficient sample processing for your protein purification & isolation applications.