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Viral Genome Sequencing With NGS
Simple detection and monitoring of viral pathogens for challenging infectious disease research
Targeted next-generation sequencing (NGS) is a rapid and accurate solution for viral genomes, suitable for viral typing, epidemiological studies, and disease surveillance using archived samples from viral investigations.
Our viral typing NGS solutions provide ultra-high sensitivity, scalability, and rapid turnaround time, which are all vital to viral monitoring and investigation research.
Ion Torrent NGS technology is based on ultrahigh-multiplex PCR, the same technology that has enabled public health agencies to closely monitor viral infections.
Benefits of using Ion Torrent NGS for studying infectious diseases include:
- Low input requirements—requires as little as 1 ng of input DNA to target regions of interest
- Fast turnaround time and automation—minimize hands-on time with automated DNA-to-insights NGS workflow, enabling you to get data from your precious samples in less than 24 hours
- Accuracy of variants
- Scalable—wide application breadth and throughput capability, all on a single platform
- Flexible panel design options—order NGS research panels for viruses such as coronavirus and Ebola virus, or use the Ion AmpliSeq Designer tool at ampliseq.com to configure your own
Unlock low-titer samples with superior sensitivity and robust performance
The Ion AmpliSeq SARS-CoV-2 Insight Research Assay leverages a unique targeted sequencing approach that yields improved coverage and uniformity from samples previously unobtainable by NGS. Incorporation of intelligent panel design provides an increased tolerance to new mutations introduced within the viral genome. Integrated analysis tools enable automated consensus sequence generation, mutation annotation, and lineage assignment.
The simplest and fastest targeted NGS workflow for infectious disease research
With Ion Torrent targeted NGS, easily go from DNA to data in less than 24 hours with ~45 minutes of hands-on time.Ion AmpliSeq libraries are prepared manually or automated with the Ion Chef System. Libraries are then placed in the Ion Chef System for emulsion PCR, enrichment, and loading onto Ion S5 Chips.
Select from pre-designed panels or configure your own with the Ion AmpliSeq Designer.
Featured research solutions:
- Coronavirus (SARS-CoV-2)
- Ebola (EBOV)
Target amplification in a single reaction
Reproducible and automated isothermal amplification
Fast and scalable next-generation sequencing
Intuitive, web-based analysis
- Torrent Suite Software—perform base calling and alignment for single samples, run plugins for secondary analysis, and easily manage your NGS data
- Ion Reporter Software—identify and annotate your variants of interest with public or private databases, and perform multi-sample comparisons
Select from pre-designed panels or configure your own with the Ion AmpliSeq Designer.
Featured research solutions:
- Coronavirus (SARS-CoV-2)
- Ebola (EBOV)
Target amplification in a single reaction
Reproducible and automated isothermal amplification
Fast and scalable next-generation sequencing
Intuitive, web-based analysis
- Torrent Suite Software—perform base calling and alignment for single samples, run plugins for secondary analysis, and easily manage your NGS data
- Ion Reporter Software—identify and annotate your variants of interest with public or private databases, and perform multi-sample comparisons
Featured panels for infectious disease research
A major challenge for microbiologists and virologists is the prediction of patterns of evolution and emergence of disease agents. RNA viruses share the biological feature of high genetic variability, which causes them to appear as clouds of mutants. Viral variants also emerge through antigenic shift within animal reservoirs like bats, snakes, pigs, and birds.
Our Ion AmpliSeq panels are based on ultrahigh-multiplex PCR, the same technology that has enabled public health agencies to closely monitor viral infections.
A few of our NGS research panels for infectious disease research are highlighted below:
Coronavirus (SARS-CoV-2) research
- Complete viral genome sequencing and variant detection of SARS-CoV-2 with >99% coverage
Ebola research
- The Ion AmpliSeq Ebola Research Panel targets 99.49% of the Ebola virus (EBOV) genome with 150 amplicons
1. Grubaugh ND, et al. Genomic epidemiology reveals multiple introductions of Zika virus into the United States. Nature. 2017 Jun 15;546(7658):401-405.
2. Cullen M, et al. Deep sequencing of HPV16 genomes: A new high-throughput tool for exploring the carcinogenicity and natural history of HPV16 infection. Papillomavirus Res. 2015 Dec 1;1:3-11.