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Flow Cytometry Protocols |
Preparing for your flow cytometry experiment
General protocols for flow cytometry
- Super Bright Staining Buffer protocol
- Cell Preparation for Flow Cytometry Protocols (Invitrogen eBioscience reagents)
- Red Blood Cell Lysis Protocols Using eBioscience Lysis Buffers (Invitrogen eBioscience reagents)
- Staining Cell Surface Targets for Flow Cytometry (Invitrogen eBioscience reagents)
- Staining Intracellular Antigens for Flow Cytometry (Invitrogen eBioscience reagents)
- OneComp and UltraComp Compensation Beads Protocols for Flow Cytometry (Invitrogen eBioscience reagents)
Cell preparation protocols for single cell suspensions
- Isolation of human peripheral blood mononuclear cells from whole blood
- Monocyte isolation from peripheral blood mononuclear cells
- NK cell isolation from peripheral blood mononuclear cells
- T cell isolation from peripheral blood mononuclear cells
- CD4+ T cell isolation from peripheral blood mononuclear cells
- CD8+ T cell isolation from peripheral blood mononuclear cells
- Regulatory T cell isolation from peripheral blood mononuclear cells
- T cell isolation from secondary lymphoid organs
- Immune cell counting using automated cell counters
- Mouse Bone Marrow Cell Isolation
- Mouse Spleen Cell Isolation Protocol
- Whole Blood Staining Protocol for Flow Cytometry Analysis
Immune cell stimulation protocols
- In vitro differentiation of macrophages from monocytes via M-CSF
- T-cell activation via functional antibodies anti-CD3 and anti-CD28
- Immune cell activation for cytokine production via LPS
- Stimulation of cytokine production in immune cells
- T-cell activation via Concanavalin A
- T-cell activation with Dynabeads Human T-Activator CD3/CD28
Cell viability protocols for flow cytometry
Cell viability and cell vitality protocols for flow cytometry
- LIVE/DEAD Violet Viability/Vitality Kit Protocol
- Annexin V Staining Protocol for Flow Cytometry (Invitrogen eBioscience reagents)
- Pharmacological Induction of Apoptosis with Camptothecin (Invitrogen eBioscience reagents)
Cell proliferation protocols for flow cytometry
- CellTrace CFSE Cell Proliferation Kit Protocol
- CellTrace Far Red Cell Proliferation Kit Protocol
- CellTrace Violet Cell Proliferation Kit Protocol
- BrdU and EdU Double Staining Protocol
- Click-iT EdU Protocol for Flow Cytometry
- Click-iT Plus EdU Flow Cytometry Assay Kits
- BrdU Staining Protocol for Flow Cytometry (Invitrogen eBioscience reagents)
- Staining Cells with eFluor Proliferation Dyes for Flow Cytometry (Invitrogen eBioscience reagents)
Cell cycle assay protocols for flow cytometry
Resources
Fluorophore and reagent selection guide for flow cytometry
Download Flow Cytometry Protocols Handbook
Spectral Flow Cytometry Fundamentals
Invitrogen eBioscience Resources—Selection guides, Best Protocols, product performance and more.
Intracellular Staining for Flow Cytometry How-To Video—for detecting cytokines and intranuclear markers.
Flow Cytometry Learning Center—Access flow cytometry educational resources for better experiment planning and execution.
Flow Cytometry Panel Builder—Design your flow cytometry panel with this online tool for a simplified, customizable experience to fit your needs.
5 Steps Resources
Support
Flow Cytometry Support Center—Find technical support recommendations for your flow cytometry workflows, including tips for experimental setup and in-depth troubleshooting help.
Flow Cytometry Panel Design Support—Work with one of our technical sales specialists to discuss your experimental needs and guide you through the process.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
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