Search Thermo Fisher Scientific
- Contáctenos
- Orden Rápida
-
¿No tiene una cuenta? Crear una cuenta
Search Thermo Fisher Scientific
The measurement of cell proliferation is fundamental to the assessment of cell health, genotoxicity, and drug efficacy. Proliferation is traditionally assessed by incubating cells with a single “pulse” of a nucleoside analog that is incorporated into DNA and detected using radioactivity, antibodies, or click chemistry. Some applications, such as drug efficacy testing, benefit from the incorporation of two different analogs at different time points (dual pulse labeling), which can further define cell cycle kinetics. With the availability of new highly specific anti-BrdU antibodies, the BrdU labeling technique can be combined with click chemistry detection for a simplified method of dual pulse labeling.
Traditionally, the detection of cell proliferation has employed the incorporation of the thymidine analog BrdU (5-bromo-2´-deoxyuridine) during DNA synthesis, followed by detection with an anti-BrdU antibody [1–4]. This method is rapidly being replaced by the click chemistry–based Click-iT EdU assay [5,6], because unlike BrdU assays, Click-iT EdU assays are not antibody-based and therefore do not require DNA denaturation for detection of the incorporated nucleoside. Click-iT assays use a modified nucleoside, EdU (5-ethynyl-2´-deoxyuridine), that is incorporated during DNA synthesis and detected using a click reaction—a copper(I)-catalyzed reaction between an azide and an alkyne.