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The cell-permeant SYTO 59 nucleic acid stain exhibits bright red fluorescence upon binding to nucleic acids. In both live and dead eukaryotic cells, SYTO 59 generally shows cytoplasmic or mitochondrial as well as nuclear staining. In addition, SYTO 59 will stain most live and permeabilized bacteria.
1. Culture cells in an appropriate medium and vessel for fluorescence microscopy. |
2. Remove the medium. |
3. Wash the cells 1–3 times in a phosphate-free buffer to remove the medium. |
4. Prepare the SYTO 59 staining solution by diluting the stock solution 1:1,000 (5 µM) in a phosphate-free buffer. |
5. Add sufficient staining solution to cover the cells. |
6. Incubate for 30 minutes, protected from light. |
7. Remove the staining solution. |
8. Wash the cells 3 times in a phosphate-free buffer. |
9. Image the cells. |
SYTO 59 | |
---|---|
Excitation/Emission (nm) | 622/645 |
Standard filter set | Cy®3.5 |
EVOS Light Cube | Texas Red |
Storage conditions | ≤–20°C |
Nuclear staining of BPAECs. BPAECs were cultured, stained with SYTO 59 dye (5 μM for 5 min), and then imaged.