Alexa Fluor 750 excitation shown as dotted line and emission shown as solid red histogram
4633Cy7752776(in buffer) 1
(in antifade) 4		microscopy, flow cytometry, fluorescence microplate reader

Invitrogen Alexa Fluor 750 dye is a bright, near-infrared fluorescent dye with excitation well suited for the 633 nm laser line or dye-pumped laser excitation. For stable signal generation in imaging and flow cytometry, Alexa Fluor 750 dye is pH-insensitive over a wide molar range. Tandem conjugates of Alexa Fluor 750 on primary and secondary antibodies are widely used in flow cytometry.

Long-wavelength emission is excellent for imaging in tissues with background autofluorescence. Alexa Fluor 750 dye is particularly useful in stochastic optical reconstruction microscopy (STORM), where it is an exceptional reporter for both dSTORM and nSTORM.

We offer Alexa Fluor 750 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to help you generate your own antibody conjugates or probes.

Protein Labeling Reagents Selection Guide (NHS ester, maleimide, etc.) Search Alexa Fluor 750 secondary antibodies

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Experimental data using Alexa Fluor 750

Flow cytometry analysis of human lymphocytes using APC-Alexa Fluor 750 dye. Human peripheral blood lymphocytes were stained using CD8 Monoclonal Antibody (3B5), APC-Alexa Fluor 750. The negative control profiles represent unstained cells.

Microscopic view of 2 colored tubulin stained with Alexa Fluor 750 dye (green) and Alexa Fluor 647 dye (magenta)

STORM imaging of immunolabeled microtubules. Tyrosinated tubulin was stained with Alexa Fluor 647 dye (magenta) and detyrosinated tubulin was stained with Alexa Fluor 750 dye (green). Square box represents zoomed region of antibody-labeled microtubules. Image courtesy of Joshua Vaughan et al., University of Washington, Seattle, WA.

3 microscopic images of mouse kidney tissues, left is labeled with Hoechst stain (blue), middle with Alexa Fluor 750 Phalloidin (red) and right is composite image

Fluorescence microscopy of mouse kidney tissues using Alexa Fluor Plus 750 Phalloidin. Cryosectioned mouse kidney tissues were labeled with NucBlue Live ReadyProbes Reagent (Hoechst 33342) and Alexa Fluor Plus 750 Phalloidin . Labeled tissues were imaged using the EVOS Cell Imaging System with an Olympus 20X Apochromat objective, EVOS Light Cube, DAPI 2.0 and EVOS Light Cube, Cy7 2.0.

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