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The eFluor 660 dye is configured as an alternative to Alexa Fluor 647 or APC. The spectral properties make it an ideal fluorophore that can be used in any flow cytometry panel incorporating the red laser.
Initial brightness | eFluor 660 dye is a high-performance fluorophore developed for use in multicolor flow cytometry. It displays stable performance when treated with formaldehyde fixation. | ||
Photostability in buffer |
633 | 660/20 | 633 | 669 | ||
Laser line | Common filter set | Excitation max | Emission max |
Effects of fixation on eFluor 660. Mouse splenocytes stained with Anti-CD4 eFluor 660 (clone GK1.5) andanalyzed immediately (dotted red line). The sample was then fixed in 2%formaldehyde overnight at 4°C (blue line) and analyzed again.
We offer eFluor 660 dye conjugated to primary antibodies for use in flow cytometry.