货号#产品名称同裂酶切割位点和识别序列突出末端最佳反应温度热灭活甲基化影响
FD0293FastDigest Eco31I
(IIS class)
BsaI, Bso31I, BspTNI
GGTCTCN^NNNN
CCAGAGNNNNN^

GGTCTCNNNNN
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: impaired if overlapped.
CpG: impaired if overlapped.
EcoKI: no effect.
FD0294FastDigest Eco31I
(IIS class)
BsaI, Bso31I, BspTNI
GGTCTCN^NNNN
CCAGAGNNNNN^

GGTCTCNNNNN
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: impaired if overlapped.
CpG: impaired if overlapped.
EcoKI: no effect.
FD0454FastDigest Esp3I
(IIS class)
BsmBI, BstGZ53I, Esp16I, Esp23I
CGTCTCN^NNNN
GCAGAGNNNNN^

CGTCTCNNNNN
5’ protruding ends37° C65°C, 10 minDam: no effect.
Dcm: no effect.
CpG: blocked if overlapped.
EcoKI: no effect.
FD1014FastDigest BpiI
(IIS class)
BbsI, BpuAI, BstV2I
GAAGACNN^NNNN
CTTCTGNNNNNN^

GAAGACNNNNNN
5’ protruding ends37° C65°C, 10 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD1074FastDigest MnlI
(IIS class)
CCTCNNNNNNN^
GGAGNNNNNN^N

CCTCNNNNNNN
3’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: blocked if overlapped.
FD0034FastDigest Alw26I
(IIS class)
BsmAI, BstMAI
GTCTCN^NNNN
CAGAGNNNNN^

GTCTCNNNNN
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD0824FastDigest MboII
(IIS class)
GAAGANNNNNNNN^
CTTCTNNNNNNN^N

GAAGANNNNNNNN
3’ protruding ends37° C65°C, 5 minDam: blocked if overlapped.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD2124FastDigest BmsI
(IIS class)
BhaI, BscAI, BscUI, BsmNI, BspST5I, Bst19I, CjeP338II, LweI, Mva1312II, PhaI, SfaNI
GCATCNNNNN^NNNN
CGTAGNNNNNNNNN^

GCATCNNNNNNNNN
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
EcoKI: no effect.
FD0964FastDigest Mva1269I
(IIS class)
BsaMI, BsmI, PctI
GAATGCN^
CTTAC^GN

GAATGCN
3’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD1934FastDigest LguI
(IIS class)
BspQI, PciSI, SapI
GCTCTTCN^NNN
CGAGAAGNNNN^

CTCTTCNNNN
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD0874FastDigest BseGI
(IIS class)
BstF5I, BtsCI, FokI
GGATGNN^
CCTAC^NN

GGATGNN
3’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD0014FastDigest AluIAluBI
AG^CT
TC^GA

AGCT
blunt37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: blocked if overlapped.
FD0024FastDigest Alw21IBbv12I, BsiHKAI
GWGCW^C
C^WCGWG

GWGCWC
3’ protruding ends37° C80°C, 20 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD0044FastDigest Alw44IApaLI, VneI
G^TGCAC
CACGT^G

GTGCAC
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD0054FastDigest BamHI
G^GATCC
CCTAG^G

GGATCC
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0055FastDigest BamHI
G^GATCC
CCTAG^G

GGATCC
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0064FastDigest BcnIAsuC2I, BpuMI, NciI
CC^SGG
GGS^CC

CCSGG
5’ protruding ends37° C80°C, 20 minDam: no effect.
Dcm: no effect.
CpG: impaired.
EcoKI: no effect.
EcoBI: no effect.
FD0074FastDigest BglI
GCCNNNN^NGGC
CGGN^NNNNCCG

GCCNNNNNGGC
3’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD0083FastDigest BglII
A^GATCT
TCTAG^A

AGATCT
5’ protruding ends37° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: impaired if overlapped.
FD0084FastDigest BglII
A^GATCT
TCTAG^A

AGATCT
5’ protruding ends37° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: impaired if overlapped.
FD0094FastDigest Bpu1102IBlpI, Bsp1720I, CelII
GC^TNAGC
CGANT^CG

GCTNAGC
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD0124FastDigest Bsp119IAsuII, Bpu14I, BspT104I, BstBI, Csp45I, NspV, SfuI
TT^CGAA
AAGC^TT

TTCGAA
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoBI: no effect.
FD0134FastDigest Bsp120IPspOMI
G^GGCCC
CCCGG^G

GGGCCC
5’ protruding ends37° C80°C, 10 minDam: no effect.
Dcm: blocked if overlapped.
CpG: blocked if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD0143FastDigest Bsu15IBanIII, Bsa29I, BseCI, BshVI, BspDI, BspXI, BsuTUI, ClaI
AT^CGAT
TAGC^TA

ATCGAT
5’ protruding ends37° C65°C, 5 minDam: blocked if overlapped.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
FD0144FastDigest Bsu15IBanIII, Bsa29I, BseCI, BshVI, BspDI, BspXI, BsuTUI, ClaI
AT^CGAT
TAGC^TA

ATCGAT
5’ protruding ends37° C65°C, 5 minDam: blocked if overlapped.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
FD0154FastDigest BsuRIBshFI, BsnI, BspANI, HaeIII, PhoI
GG^CC
CC^GG

GGCC
blunt37° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0184FastDigest Cfr10IBse118I, BsrFI, BssAI
R^CCGGY
YGGCC^R

RCCGGY
5’ protruding ends37° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD0194FastDigest Cfr13IAspS9I, BmgT120I, PspPI, Sau96I
G^GNCC
CCNG^G

GGNCC
5’ protruding ends37° CNo
(column purification)
Dam: no effect.
Dcm: blocked if overlapped.
CpG: blocked if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD0214FastDigest Csp6IAfaI, CviQI, RsaNI
G^TAC
CAT^G

GTAC
5’ protruding ends37° C80°C, 10 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0224FastDigest DraI
TTT^AAA
AAA^TTT

TTTAAA
blunt37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: blocked if overlapped.
EcoBI: no effect.
FD0234FastDigest Eam1104IBst6I, EarI
CTCTTCN^NNN
GAGAAGNNNN^

CTCTTCNNNN
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD0244FastDigest Eam1105IAhdI, AspEI, BmeRI, DriI, EclHKI
GACNNN^NNGTC
CTGNN^NNNCAG

GACNNNNNGTC
3’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0254FastDigest Ecl136IIEco53kI, EcoICRI, Psp124BI, SstI
GAG^CTC
CTC^GAG

GAGCTC
blunt37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
EcoKI: no effect.
FD0264FastDigest EcoO109IDraII
RG^GNCCY
YCCNG^GR

RGGNCCY
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: blocked if overlapped.
CpG: no effect.
EcoKI: no effect.
FD0274FastDigest EcoRI
G^AATTC
CTTAA^G

GAATTC
5’ protruding ends37° C80°C, 5minDam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD0275FastDigest EcoRI
G^AATTC
CTTAA^G

GAATTC
5’ protruding ends37° C80°C, 5minDam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD0303FastDigest Eco32IEcoRV
GAT^ATC
CTA^TAG

GATATC
blunt37° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD0304FastDigest Eco32IEcoRV
GAT^ATC
CTA^TAG

GATATC
blunt37° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD0314FastDigest Eco47IAvaII, Bme18I, SinI, VpaK11BI
G^GWCC
CCWG^G

GGWCC
5’ protruding ends37° C80°C, 20 minDam: no effect.
Dcm: blocked if overlapped.
CpG: blocked if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD0324FastDigest Eco47IIIAfeI, Aor51HI
AGC^GCT
TCG^CGA

AGCGCT
blunt37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
FD0334FastDigest Eco52IBseX3I, BstZI, EagI, EclXI
C^GGCCG
GCCGG^C

CGGCCG
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD0344FastDigest Eco57IAcuI, Bsp6II, BspD6II, BspKT5I, Eco112I, Eco125I, FsfI, SstE37III
CTGAAGNNNNNNNNNNNNNNNN^
GACTTCNNNNNNNNNNNNNN^NN

CTGAAGNNNNNNNNNNNNNNNN
3’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD0364FastDigest Eco72IAcvI, BbrPI, PmaCI, PmlI, PspCI
CAC^GTG
GTG^CAC

CACGTG
blunt37° C80°C, 10 minDam: no effect.
Dcm: no effect.
CpG: blocked.
FD0374FastDigest Eco81IAxyI, Bse21I, Bsu36I
CC^TNAGG
GGANT^CC

CCTNAGG
5’ protruding ends37° C80°C, 10 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD0384FastDigest Eco88IAma87I, AvaI, BmeT110I, BsiHKCI, BsoBIm
C^YCGRG
GRGCY^C

CYCGRG
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: impaired.
EcoKI: no effect.
EcoBI: no effect.
FD0394FastDigest Eco91IBstEII, BstPI, EcoO65I, PspEI
G^GTNACC
CCANTG^G

GGTNACC
5’ protruding ends37° C65°C, 10 minDam: no effect.
Dcm: no effect.
CpG: no effect.
FD0404FastDigest Eco105IBstSNI, SnaBI
TAC^GTA
ATG^CAT

TACGTA
blunt37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD0414FastDigest Eco130IBssT1I, EcoT14I, ErhI, StyI
C^CWWGG
GGWWC^C

CCWWGG
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0424FastDigest Eco147IAatI, PceI, SseBI, StuI
AGG^CCT
TCC^GGA

AGGCCT
blunt37° C80°C, 10 minDam: no effect.
Dcm: blocked if overlapped.
CpG: no effect.
EcoKI: no effect.
FD0434FastDigest ScaIAssI, BmcAI, ZrmI
AGT^ACT
TCA^TGA

AGTACT
blunt37° C65°C, 10 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: blocked if overlapped.
FD0443FastDigest EheIBbeI, DinI, EgeI, KasI, Mly113I, NarI, SfoI
GGC^GCC
CCG^CGG

GGCGCC
blunt37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD0444FastDigest EheIBbeI, DinI, EgeI, KasI, Mly113I, NarI, SfoI
GGC^GCC
CCG^CGG

GGCGCC
blunt37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD0464FastDigest GsuIBco35I, BpmI, Bsp22I, Bsp28I, BspJ74I, Bth1795I, Uba1437I, Uba1444I
CTGGAGNNNNNNNNNNNNNNNN^
GACCTCNNNNNNNNNNNNNN^NN

CTGGAGNNNNNNNNNNNNNNNN
3’ protruding ends30° C65°C, 5 minDam: no effect.
Dcm: blocked if overlapped.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0474FastDigest Hin1IAcyI, BsaHI, BssNI, BstACI, Hsp92I
GR^CGYC
CYGC^RG

GRCGYC
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: impaired if overlapped.
CpG: blocked.
EcoKI: no effect.
FD0484FastDigest Hin6IAspLEI, BstHHI, CfoI, HinP1I, HspAI
G^CGC
CGC^G

GCGC
5’ protruding ends37° C80°C, 10 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD0494FastDigest HincIIHindII
GTY^RAC
CAR^YTG

GTYRAC
blunt37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
EcoKI: blocked if overlapped.
EcoBI: blocked if overlapped.
FD0504FastDigest HindIII
A^AGCTT
TTCGA^A

AAGCTT
5’ protruding ends37° C80°C, 10 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: impaired if overlapped.
FD0505FastDigest HindIII
A^AGCTT
TTCGA^A

AAGCTT
5’ protruding ends37° C80°C, 10 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD0514FastDigest HpaIIBsiSI, HapII
C^CGG
GGC^C

CCGG
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD0524FastDigest KpnIAsp718I
GGTAC^C
C^CATGG

GGTACC
3’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0534FastDigest Kpn2IAccIII, Aor13HI, BseAI, Bsp13I, BspEI, MroI
T^CCGGA
AGGCC^T

TCCGGA
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD0544FastDigest MspIBsiSI, HapII
C^CGG
GGC^C

CCGG
5’ protruding ends37° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0554FastDigest MvaIAjnI, BseBI, Bst2UI, BstNI, BstOI, Psp6I, PspGI
CC^WGG
GGW^CC

CCWGG
5’ protruding ends37° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0564FastDigest MluI
A^CGCGT
TGCGC^A

ACGCGT
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD0573FastDigest NcoIBsp19I
C^CATGG
GGTAC^C

CCATGG
5’ protruding ends37° C65°C, 15 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0574FastDigest NcoIBsp19I
C^CATGG
GGTAC^C

CCATGG
5’ protruding ends37° C65°C, 15 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0575FastDigest NcoIBsp19I
C^CATGG
GGTAC^C

CCATGG
5’ protruding ends37° C65°C, 15 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0583FastDigest NdeIFauNDI
CA^TATG
GTAT^AC

CATATG
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0584FastDigest NdeIFauNDI
CA^TATG
GTAT^AC

CATATG
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0585FastDigest NdeIFauNDI
CA^TATG
GTAT^AC

CATATG
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0593FastDigest NotICciNI
GC^GGCCGC
CGCCGG^CG

GCGGCCGC
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD0594FastDigest NotICciNI
GC^GGCCGC
CGCCGG^CG

GCGGCCGC
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD0595FastDigest NotICciNI
GC^GGCCGC
CGCCGG^CG

GCGGCCGC
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD0596FastDigest NotICciNI
GC^GGCCGC
CGCCGG^CG

GCGGCCGC
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD0604FastDigest PaeIBbuI, SphI
GCATG^C
C^GTACG

GCATGC
3’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: blocked if overlapped.
FD0614FastDigest PstIBspMAI
CTGCA^G
G^ACGTC

CTGCAG
3’ protruding ends37° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0615FastDigest PstIBspMAI
CTGCA^G
G^ACGTC

CTGCAG
3’ protruding ends37° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0624FastDigest PvuIBpvUI, MvrI, Ple19I
CGAT^CG
GC^TAGC

CGATCG
3’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD0634FastDigest PvuII
CAG^CTG
GTC^GAC

CAGCTG
blunt37° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD0644FastDigest SalI
G^TCGAC
CAGCT^G

GTCGAC
5’ protruding ends37° C65°C, 10 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD0654FastDigest SduIBsp1286I, MhlI
GDGCH^C
C^HCGDG

GDGCHC
3’ protruding ends37° C80°C, 15 minDam: no effect.
Dcm: no effect.
CpG: no effect.
FD0663FastDigest SmaITspMI, XmaCI, XmaI
CCC^GGG
GGG^CCC

CCCGGG
blunt37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD0664FastDigest SmaITspMI, XmaCI, XmaI
CCC^GGG
GGG^CCC

CCCGGG
blunt37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD0674FastDigest TaqI
T^CGA
AGC^T

TCGA
5’ protruding ends65° CNo
(column purification)
Dam: blocked if overlapped.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0684FastDigest XbaI
T^CTAGA
AGATC^T

TCTAGA
5’ protruding ends37° C65°C, 20 minDam: blocked if overlapped.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0685FastDigest XbaI
T^CTAGA
AGATC^T

TCTAGA
5’ protruding ends37° C65°C, 20 minDam: blocked if overlapped.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0694FastDigest XhoIPaeR7I, Sfr274I, SlaI, StrI, TliI
C^TCGAG
GAGCT^C

CTCGAG
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: impaired.
EcoKI: no effect.
EcoBI: no effect.
FD0695FastDigest XhoIPaeR7I, Sfr274I, SlaI, StrI, TliI
C^TCGAG
GAGCT^C

CTCGAG
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: impaired.
EcoKI: no effect.
EcoBI: no effect.
FD0704FastDigest Bst1107IBssNAI, BstZ17I
GTA^TAC
CAT^ATG

GTATAC
blunt37° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD0714FastDigest Van91IAccB7I, BasI, PflMI
CCANNNN^NTGG
GGTN^NNNNACC

CCANNNNNTGG
3’ protruding ends37° C65°C, 10 minDam: no effect.
Dcm: blocked if overlapped.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0724FastDigest BclIFbaI, Ksp22I
T^GATCA
ACTAG^T

TGATCA
5’ protruding ends37° CNo
(column purification)
Dam: blocked.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: blocked if overlapped.
FD0734FastDigest Mph1103IEcoT22I, NsiI, Zsp2I
ATGCA^T
T^ACGTA

ATGCAT
3’ protruding ends37° C65°C, 15 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD0744FastDigest CpoICspI, Rsr2I, RsrII
CG^GWCCG
GCCWG^GC

CGGWCCG
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD0753FastDigest MunIMfeI
C^AATTG
GTTAA^C

CAATTG
5’ protruding ends37° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD0754FastDigest MunIMfeI
C^AATTG
GTTAA^C

CAATTG
5’ protruding ends37° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD0764FastDigest Psp5IIPpuMI, PspPPI
RG^GWCCY
YCCWG^GR

RGGWCCY
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: blocked if overlapped.
CpG: no effect.
EcoKI: no effect.
FD0774FastDigest SspI
AAT^ATT
TTA^TAA

AATATT
blunt37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0784FastDigest Bsp143IBfuCI, BssMI, BstKTI, BstMBI, DpnII, Kzo9I, NdeII, Sau3AIm
^GATC
CTAG^

GATC
5’ protruding ends37° C65°C, 20 minDam: no effect.
Dcm: no effect.
CpG: blocked if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD0804FastDigest HinfI
G^ANTC
CTNA^G

GANTC
5’ protruding ends37° C65°C, 20 minDam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
EcoKI: no effect.
EcoBI: blocked if overlapped.
FD0814FastDigest MboIBfuCI, BssMI, BstKTI, BstMBI, DpnII, Kzo9I, NdeII, Sau3AIm
^GATC
CTAG^

GATC
5’ protruding ends37° C65°C, 15 minDam: blocked.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: blocked if overlapped.
FD0834FastDigest BspTIAflII, BfrI, Bst98I, BstAFI, MspCI, Vha464I
C^TTAAG
GAATT^C

CTTAAG
5’ protruding ends37° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0854FastDigest Pfl23IIBsiWI, PspLI
C^GTACG
GCATG^C

CGTACG
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD0884FastDigest BseNIBse1I, BsrI, BsrSI
ACTGGN^
TGAC^CN

ACTGGN
3’ protruding ends65° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
FD0894FastDigest Bsh1285IBsiEI, BstMCI
CGRY^CG
GC^YRGC

CGRYCG
3’ protruding ends37° C80°C, 15 minDam: impaired if overlapped.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD0904FastDigest Acc65IAsp718I
G^GTACC
CCATG^G

GGTACC
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: impaired if overlapped.
CpG: impaired if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD0914FastDigest VspIAseI, PshBI
AT^TAAT
TAAT^TA

ATTAAT
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD0924FastDigest Bsh1236IAccII, BspFNI, BstFNI, BstUI, MvnI
CG^CG
GC^GC

CGCG
blunt37° C80°C, 10 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD0933FastDigest Bsp1407IBsrGI, BstAUI
T^GTACA
ACATG^T

TGTACA
5’ protruding ends37° C80°C, 10 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0934FastDigest Bsp1407IBsrGI, BstAUI
T^GTACA
ACATG^T

TGTACA
5’ protruding ends37° C80°C, 10 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0944FastDigest Psp1406IAclI
AA^CGTT
TTGC^AA

AACGTT
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
FD0973FastDigest NheIAsuNHI, BmtI
G^CTAGC
CGATC^G

GCTAGC
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD0974FastDigest NheIAsuNHI, BmtI
G^CTAGC
CGATC^G

GCTAGC
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD0984FastDigest Tru1IMseI, Tru9I
T^TAA
AAT^T

TTAA
5’ protruding ends65° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: blocked if overlapped.
EcoBI: no effect.
FD0994FastDigest AatIIZraI
GACGT^C
C^TGCAG

GACGTC
3’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD1004FastDigest BshNIAccB1I, BanI, BspT107I
G^GYRCC
CCRYG^G

GGYRCC
5’ protruding ends37° C65°C, 10 minDam: no effect.
Dcm: impaired if overlapped.
CpG: impaired if overlapped.
EcoBI: no effect.
FD1024FastDigest BstXI
CCANNNNN^NTGG
GGTN^NNNNNACC

CCANNNNNNTGG
3’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: impaired if overlapped.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD1034FastDigest KspAIHpaI
GTT^AAC
CAA^TTG

GTTAAC
blunt37° C65°C, 20 minDam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
EcoKI: blocked if overlapped.
EcoBI: no effect.
FD1084FastDigest BseDIBsaJI, BssECI
C^CNNGG
GGNNC^C

CCNNGG
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD1124FastDigest RsaIAfaI, CviQI, RsaNI
GT^AC
CA^TG

GTAC
blunt37° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD1133FastDigest SacIEco53kI, EcoICRI, Psp124BI, SstI
GAGCT^C
C^TCGAG

GAGCTC
3’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD1134FastDigest SacIEco53kI, EcoICRI, Psp124BI, SstI
GAGCT^C
C^TCGAG

GAGCTC
3’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD1144FastDigest TaiIHpyCH4IV, MaeII
ACGT^
^TGCA

ACGT
3’ protruding ends65° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: blocked.
FD1154FastDigest BspLIBmiI, NlaIV, PspN4I
GGN^NCC
CCN^NGG

GGNNCC
blunt37° C65°C, 20 minDam: no effect.
Dcm: impaired if overlapped.
CpG: impaired if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD1164FastDigest BfmIBstSFI, SfcI
C^TRYAG
GAYRT^C

CTRYAG
5’ protruding ends37° C65°C, 20 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD1184FastDigest Bpu10I
CC^TNAGC
GGANT^CG

CCTNAGC
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD1194FastDigest SdaISbfI, Sse8387I
CCTGCA^GG
GG^ACGTCC

CCTGCAGG
3’ protruding ends37° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD1204FastDigest BseLIAfiI, Bsc4I, BslI
CCNNNNN^NNGG
GGNN^NNNNNCC

CCNNNNNNNGG
3’ protruding ends37° CNo
(column purification)
Dam: no effect.
Dcm: impaired if overlapped.
CpG: impaired if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD1214FastDigest MlsIBalI, MluNI, MscI, Msp20I
TGG^CCA
ACC^GGT

TGGCCA
blunt37° C80°C, 20 minDam: no effect.
Dcm: blocked if overlapped.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD1224FastDigest NsbIAcc16I, AviII, FspI
TGC^GCA
ACG^CGT

TGCGCA
blunt37° C65°C, 15 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD1234FastDigest AdeIDraIII
CACNNN^GTG
GTG^NNNCAC

CACNNNGTG
3’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
FD1244FastDigest SmiISwaI
ATTT^AAAT
TAAA^TTTA

ATTTAAAT
blunt37° C65°C, 15 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD1253FastDigest BcuIAhlI, SpeI
A^CTAGT
TGATC^A

ACTAGT
5’ protruding ends37° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: no effect.
FD1254FastDigest BcuIAhlI, SpeI
A^CTAGT
TGATC^A

ACTAGT
5’ protruding ends37° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: no effect.
FD1264FastDigest BseMIBse3DI, BsrDI
GCAATGNN^
CGTTAC^NN

GCAATGNN
3’ protruding ends55° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD1274FastDigest MbiIAccBSI, BsrBI
CCG^CTC
GGC^GAG

CCGCTC
blunt37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: impaired.
EcoKI: no effect.
FD1284FastDigest PagICciI, BspHI, RcaI
T^CATGA
AGTAC^T

TCATGA
5’ protruding ends37° C80°C, 5 minDam: impaired if overlapped.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: impaired if overlapped.
FD1294FastDigest TatI
W^GTACW
WCATG^W

WGTACW
5’ protruding ends65° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD1304FastDigest XagIBstENI, EcoNI
CCTNN^NNNAGG
GGANNN^NNTCC

CCTNNNNNAGG
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD1314FastDigest BplI
GAAGACNN^NNNN
CTTCTGNNNNNN^

GAAGACNNNNNN
3’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD1334FastDigest PsyIAspI, PflFI, Tth111I
GACN^NNGTC
CTGNN^NCAG

GACNNNGTC
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD1344FastDigest MssIPmeI
GTTT^AAAC
CAAA^TTTG

GTTT^AAAC
blunt37° C65°C, 10 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: blocked if overlapped.
EcoBI: no effect.
FD1354FastDigest TasISse9I, Tsp509I, TspEI
^AATT
TTAA^>

AATT
5’ protruding ends65° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: blocked if overlapped.
FD1364FastDigest TaaIBst4CI, HpyCH4III
ACN^GT
TG^NCA

ACNGT
3’ protruding ends65° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
FD1374FastDigest SchIMlyI, PleI, PpsI, SchI
GAGTCNNNNN^
CTCAGNNNNN

GAGTCNNNNN
blunt37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD1383FastDigest XapIAcsI, ApoI
R^AATTY
YTTAA^R

RAATTY
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD1384FastDigest XapIAcsI, ApoI
R^AATTY
YTTAA^R

RAATTY
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD1394FastDigest CaiIAlwNI
CAGNNN^CTG
GTC^NNNGAC

CAGNNNCTG
3’ protruding ends37° C65°C, 10 minDam: no effect.
Dcm: blocked if overlapped.
CpG: no effect.
EcoKI: no effect.
FD1404FastDigest BseMIIBspCNI
GCAATGNN^
CGTTAC^NN

GCAATGNN
3’ protruding ends55° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD1414FastDigest ApaIPspOMI
GGGCC^C
C^CCGGG

GGGCCC
3’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: impaired if overlapped.
CpG: impaired if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD1424FastDigest Bme1390IBmrFI, BssKI, BstSCI, MspR9I, ScrFI, StyD4I
CC^NGG
GGN^CC

CCNGG
5’ protruding ends37° CNo
(column purification)
Dam: no effect.
Dcm: blocked if overlapped.
CpG: blocked if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD1434FastDigest BoxIBstPAI, PshAI
GACNN^NNGTC
CTGNN^NNCAG

GACNNNNGTC
blunt37° C80°C, 20 minDam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
EcoKI: no effect.
FD1444FastDigest BseSIBaeGI, BstSLI
GKGCM^C
C^MCGKG

GKGCMC
3’ protruding ends37° C80°C, 15 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: impaired if overlapped.
EcoBI: no effect.
FD1454FastDigest BseXIBbvI, BstV1I
GCAGCNNNNNNNN^NNNN
CGTCGNNNNNNNNNNNN^

GCAGCNNNNNNNNNNNN
5’ protruding ends65° C80°C, 15 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD1464FastDigest BshTIAgeI, AsiGI, CspAI, PinAI
A^CCGGT
TGGCC^A

ACCGGT
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
FD1474FastDigest XceIBstNSI, NspI
RCATG^Y
Y^GTACR

RCATGY
3’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD1484FastDigest XmiIAccI, FblI
GT^MKAC
CAKM^TG

GTMKAC
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD1514FastDigest NmuCITsp45I
^GTSAC
CASTG^

GTSAC
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
EcoKI: no effect.
FD1524FastDigest PdiIMroNI, NaeI, NgoMIV
GCC^GGC
CGG^CCG

GCCGGC
blunt37° C65°C, 15 minDam: no effect.
Dcm: no effect.
CpG: blocked if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD1534FastDigest PdmIAsp700I, MroXI, XmnI
GAANN^NNTTC
CTTNN^NNAAG

GAANNNNTTC
blunt37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
FD1554FastDigest PsuIBstX2I, BstYI, MflI, XhoII
R^GATCY
YCTAG^R

RGATCY
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD1564FastDigest XmaJIAspA2I, AvrII, BlnI
C^CTAGG
GGATC^C

CCTAGG
5’ protruding ends37° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD1574FastDigest Hpy8IHpy166II
GTN^NAC
CAN^NTG

GTNNAC
blunt37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
FD1634FastDigest OliIAleI
CACNN^NNGTG
GTGNN^NNCAC

CACNNNNGTG
blunt37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
EcoKI: blocked if overlapped.
EcoBI: blocked if overlapped.
FD1644FastDigest SatIFnu4HI, Fsp4HI, ItaI
GC^NGC
CGN^CG

GCNGC
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD1654FastDigest TauI
GCSG^C
C^GSCG

GCSGC
3’ protruding ends55° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD1664FastDigest FspAI
RTGC^GCAY
YACG^CGTR

RTGCGCAY
blunt37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
FD1703FastDigest DpnIMalI
GA^TC
CT^AG

GATC
blunt37° C80°C, 5 minDam: does not cut dam- DNA.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD1704FastDigest DpnIMalI
GA^TC
CT^AG

GATC
blunt37° C80°C, 5 minDam: does not cut dam - DNA.
Dcm: no effect.
Cpg: no effect.
EcoKI: no effect.
FD1714FastDigest BseJIBsaBI, Bse8I
GATNN^NNATC
CTANN^NNTAG

GATNNNNATC
blunt37° CNo
(column purification)
Dam: blocked if overlapped.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD1724FastDigest AasIDrdI, DseDI
GACNNNN^NNGTC
CTGNN^NNNNCAG

GACNNNNNNGTC
3’ protruding ends37° C80°C, 10 minDam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD1734FastDigest HpyF10VIBstMWI, MwoI
GCNNNNN^NNGC
CGNN^NNNNNCG

GCNNNNNNNGC
3’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD1744FastDigest BveIAcc36I, BfuAI, BspMI
ACCTGCNNNN^NNNN
TGGACGNNNNNNNN^

ACCTGCNNNNNNNN
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
FD1754FastDigest PfoI
T^CCNGGA
AGGNCC^T

TCCNGGA
5’ protruding ends37° C65°C, 5 minDam: impaired if overlapped.
Dcm: blocked if overlapped.
CpG: blocked if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD1764FastDigest FspBIBfaI, MaeI, XspI
C^TAG
GAT^C

CTAG
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD1784FastDigest PfeITfiI
G^AWTC
CTWA^G

GAWTC
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked if overlapped.
EcoKI: no effect.
FD1794FastDigest SsiIAciI, BspACI
C^CGC
GGC^G

CCGC
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD1814FastDigest FaqIBslFI, BsmFI
GGGACNNNNNNNNNN^NNNN
CCCTGNNNNNNNNNNNNNN^

GGGACNNNNNNNNNNNNNN
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD1824FastDigest SfiI
GGCCNNNN^NGGCC
CCGGN^NNNNCCGG

GGCCNNNNNGGCC
3’ protruding ends50° CNo
(column purification)
Dam: no effect.
Dcm: impaired if overlapped.
CpG: impaired if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD1834FastDigest Hin1IICviAII, FaeI, FatI, Hsp92II, NlaIII
CATG^
^GTAC

CATG
3’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD1854FastDigest HhaIAspLEI, BstHHI, CfoI, HinP1I, HspAI
GCG^C
C^GCG

GCGC
3’ protruding ends37° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD1884FastDigest HpyF3IBstDEI, DdeI
C^TNAG
GANT^C

CTNAG
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD1894FastDigest SgsIAscI, PalAI
GG^CGCGCC
CCGCGC^GG

GGCGCGCC
5’ protruding ends37° C65°C, 20 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD1904FastDigest CseIHgaI
GACGCNNNNN^NNNNN
CTGCGNNNNNNNNNN^

GACGCNNNNNNNNNN
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
FD1954FastDigest AjuI
NNNNN^NNNNNNNGAANNNNNNNTTGGNNNNNNNNNNN^
^NNNNNNNNNNNNCTTNNNNNNNAACCNNNNNN^NNNNN

NNNNNNNNNNNNGAANNNNNNNTTGGNNNNNNNNNNN
3’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD1974FastDigest Ppu21IBsaAI, BstBAI
YAC^GTR
RTG^CAY

YACGTR
blunt37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked if overlapped.
FD2004FastDigest RseIMslI, SmiMI
CAYNN^NNRTG
GTRNN^NNYAC

CAYNNNNRTG
blunt37° C65°C, 20 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: blocked if overlapped.
FD2024FastDigest MreI
CG^CCGGCG
GCGGCC^GC

CGCCGGCG
3’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD2044FastDigest BspOIAsuNHI, BmtI
GCTAG^C
C^GATCG

GCTAGC
3’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD2064FastDigest AanIPsiI
TTA^TAA
AAT^ATT

TTATAA
blunt37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoBI: no effect.
FD2074FastDigest Lsp1109IBbvI, BstV1I
GCAGCNNNNNNNN^NNNN
CGTCGNNNNNNNNNNNN^

GCAGCNNNNNNNNNNNN
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD2084FastDigest MauBI
CG^CGCGCG
GCGCGC^GC

CGCGCGCG
3’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD2094FastDigest SfaAIAsiSI, RgaI, SgfI
GCGAT^CGC
CGC^TAGCG

GCGATCGC
3’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD2104FastDigest TscAITspRI
NNCASTGNN^
^NNGTSACNN

NNCASTGNN
3’ protruding ends65° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: no effect.
FD2114FastDigest CsiIMabI, SexAI
A^CCWGGT
TGGWCC^A

ACCWGGT
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
FD2134FastDigest PteIBsePIm BssHII
G^CGCGC
CGCGC^G

GCGCGC
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD2144FastDigest FokIBstF5I, BtsCI, FokI
GGATGNNNNNNNNN^NNNN
CCTACNNNNNNNNNNNNN^

GGATGNNNNNNNNNNNNN
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD2154FastDigest RruIBtuMI, NruI
TCG^CGA
AGC^GCT

TCGCGA
blunt37° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD2164FastDigest KflISse1825I, SanDI
GG^GWCCC
CCCWG^GG

GGGWCCC
5’ protruding ends37° CNo
(column purification)
Dam: no effect.
CpG: blocked if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD2174FastDigest SaqAIMseI, Tru1I, Tru9I
T^TAA
AAT^T

TTAA
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoBI: no effect.
FD2184FastDigest BfoIAccB2I, Bme142I, BsmHI, Bsp143II, Bst16I, Bst1473II, BstH2I, Btu34II, HaeII, HinHI, LpnI, NgoAI, NgoBI, NgoCI, NgoGI, NgoJI, NgoMI, NgoWI
RGCGC^Y
Y^CGCGR

RGCGCY
3’ protruding ends37° C65°C, 10 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
FD2204FastDigest PacI
TTAAT^TAA
AAT^TAATT

TTAATTAA
3’ protruding ends37° C65°C, 10 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoBI: no effect.
FD2224FastDigest PspFI
CCCAG^C
G^GGTCG

CCCAGC
3’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
EcoKI: no effect.
EcoBI: no effect.

仅供科研使用,不可用于诊断目的。