Search Thermo Fisher Scientific
| 货号# | 产品名称 | 同裂酶 | 切割位点和识别序列 | 突出末端 | 最佳反应温度 | 热灭活 | 甲基化影响 |
|---|---|---|---|---|---|---|---|
| FD0293 | FastDigest Eco31I (IIS class) | BsaI, Bso31I, BspTNI | GGTCTCN^NNNN CCAGAGNNNNN^ GGTCTCNNNNN | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: impaired if overlapped. CpG: impaired if overlapped. EcoKI: no effect. |
| FD0294 | FastDigest Eco31I (IIS class) | BsaI, Bso31I, BspTNI | GGTCTCN^NNNN CCAGAGNNNNN^ GGTCTCNNNNN | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: impaired if overlapped. CpG: impaired if overlapped. EcoKI: no effect. |
| FD0454 | FastDigest Esp3I (IIS class) | BsmBI, BstGZ53I, Esp16I, Esp23I | CGTCTCN^NNNN GCAGAGNNNNN^ CGTCTCNNNNN | 5’ protruding ends | 37° C | 65°C, 10 min | Dam: no effect. Dcm: no effect. CpG: blocked if overlapped. EcoKI: no effect. |
| FD1014 | FastDigest BpiI (IIS class) | BbsI, BpuAI, BstV2I | GAAGACNN^NNNN CTTCTGNNNNNN^ GAAGACNNNNNN | 5’ protruding ends | 37° C | 65°C, 10 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD1074 | FastDigest MnlI (IIS class) | CCTCNNNNNNN^ GGAGNNNNNN^N CCTCNNNNNNN | 3’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: blocked if overlapped. | |
| FD0034 | FastDigest Alw26I (IIS class) | BsmAI, BstMAI | GTCTCN^NNNN CAGAGNNNNN^ GTCTCNNNNN | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect. |
| FD0824 | FastDigest MboII (IIS class) | GAAGANNNNNNNN^ CTTCTNNNNNNN^N GAAGANNNNNNNN | 3’ protruding ends | 37° C | 65°C, 5 min | Dam: blocked if overlapped. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. | |
| FD2124 | FastDigest BmsI (IIS class) | BhaI, BscAI, BscUI, BsmNI, BspST5I, Bst19I, CjeP338II, LweI, Mva1312II, PhaI, SfaNI | GCATCNNNNN^NNNN CGTAGNNNNNNNNN^ GCATCNNNNNNNNN | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. |
| FD0964 | FastDigest Mva1269I (IIS class) | BsaMI, BsmI, PctI | GAATGCN^ CTTAC^GN GAATGCN | 3’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD1934 | FastDigest LguI (IIS class) | BspQI, PciSI, SapI | GCTCTTCN^NNN CGAGAAGNNNN^ CTCTTCNNNN | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD0874 | FastDigest BseGI (IIS class) | BstF5I, BtsCI, FokI | GGATGNN^ CCTAC^NN GGATGNN | 3’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD0014 | FastDigest AluI | AluBI | AG^CT TC^GA AGCT | blunt | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: blocked if overlapped. |
| FD0024 | FastDigest Alw21I | Bbv12I, BsiHKAI | GWGCW^C C^WCGWG GWGCWC | 3’ protruding ends | 37° C | 80°C, 20 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD0044 | FastDigest Alw44I | ApaLI, VneI | G^TGCAC CACGT^G GTGCAC | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked if overlapped. EcoKI: no effect. EcoBI: no effect. |
| FD0054 | FastDigest BamHI | G^GATCC CCTAG^G GGATCC | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. | |
| FD0055 | FastDigest BamHI | G^GATCC CCTAG^G GGATCC | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. | |
| FD0064 | FastDigest BcnI | AsuC2I, BpuMI, NciI | CC^SGG GGS^CC CCSGG | 5’ protruding ends | 37° C | 80°C, 20 min | Dam: no effect. Dcm: no effect. CpG: impaired. EcoKI: no effect. EcoBI: no effect. |
| FD0074 | FastDigest BglI | GCCNNNN^NGGC CGGN^NNNNCCG GCCNNNNNGGC | 3’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect. | |
| FD0083 | FastDigest BglII | A^GATCT TCTAG^A AGATCT | 5’ protruding ends | 37° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: impaired if overlapped. | |
| FD0084 | FastDigest BglII | A^GATCT TCTAG^A AGATCT | 5’ protruding ends | 37° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: impaired if overlapped. | |
| FD0094 | FastDigest Bpu1102I | BlpI, Bsp1720I, CelII | GC^TNAGC CGANT^CG GCTNAGC | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD0124 | FastDigest Bsp119I | AsuII, Bpu14I, BspT104I, BstBI, Csp45I, NspV, SfuI | TT^CGAA AAGC^TT TTCGAA | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoBI: no effect. |
| FD0134 | FastDigest Bsp120I | PspOMI | G^GGCCC CCCGG^G GGGCCC | 5’ protruding ends | 37° C | 80°C, 10 min | Dam: no effect. Dcm: blocked if overlapped. CpG: blocked if overlapped. EcoKI: no effect. EcoBI: no effect. |
| FD0143 | FastDigest Bsu15I | BanIII, Bsa29I, BseCI, BshVI, BspDI, BspXI, BsuTUI, ClaI | AT^CGAT TAGC^TA ATCGAT | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: blocked if overlapped. Dcm: no effect. CpG: blocked. EcoKI: no effect. |
| FD0144 | FastDigest Bsu15I | BanIII, Bsa29I, BseCI, BshVI, BspDI, BspXI, BsuTUI, ClaI | AT^CGAT TAGC^TA ATCGAT | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: blocked if overlapped. Dcm: no effect. CpG: blocked. EcoKI: no effect. |
| FD0154 | FastDigest BsuRI | BshFI, BsnI, BspANI, HaeIII, PhoI | GG^CC CC^GG GGCC | blunt | 37° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD0184 | FastDigest Cfr10I | Bse118I, BsrFI, BssAI | R^CCGGY YGGCC^R RCCGGY | 5’ protruding ends | 37° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD0194 | FastDigest Cfr13I | AspS9I, BmgT120I, PspPI, Sau96I | G^GNCC CCNG^G GGNCC | 5’ protruding ends | 37° C | No (column purification) | Dam: no effect. Dcm: blocked if overlapped. CpG: blocked if overlapped. EcoKI: no effect. EcoBI: no effect. |
| FD0214 | FastDigest Csp6I | AfaI, CviQI, RsaNI | G^TAC CAT^G GTAC | 5’ protruding ends | 37° C | 80°C, 10 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD0224 | FastDigest DraI | TTT^AAA AAA^TTT TTTAAA | blunt | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: blocked if overlapped. EcoBI: no effect. | |
| FD0234 | FastDigest Eam1104I | Bst6I, EarI | CTCTTCN^NNN GAGAAGNNNN^ CTCTTCNNNN | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD0244 | FastDigest Eam1105I | AhdI, AspEI, BmeRI, DriI, EclHKI | GACNNN^NNGTC CTGNN^NNNCAG GACNNNNNGTC | 3’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD0254 | FastDigest Ecl136II | Eco53kI, EcoICRI, Psp124BI, SstI | GAG^CTC CTC^GAG GAGCTC | blunt | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. |
| FD0264 | FastDigest EcoO109I | DraII | RG^GNCCY YCCNG^GR RGGNCCY | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: blocked if overlapped. CpG: no effect. EcoKI: no effect. |
| FD0274 | FastDigest EcoRI | G^AATTC CTTAA^G GAATTC | 5’ protruding ends | 37° C | 80°C, 5min | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect. | |
| FD0275 | FastDigest EcoRI | G^AATTC CTTAA^G GAATTC | 5’ protruding ends | 37° C | 80°C, 5min | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect. | |
| FD0303 | FastDigest Eco32I | EcoRV | GAT^ATC CTA^TAG GATATC | blunt | 37° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD0304 | FastDigest Eco32I | EcoRV | GAT^ATC CTA^TAG GATATC | blunt | 37° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD0314 | FastDigest Eco47I | AvaII, Bme18I, SinI, VpaK11BI | G^GWCC CCWG^G GGWCC | 5’ protruding ends | 37° C | 80°C, 20 min | Dam: no effect. Dcm: blocked if overlapped. CpG: blocked if overlapped. EcoKI: no effect. EcoBI: no effect. |
| FD0324 | FastDigest Eco47III | AfeI, Aor51HI | AGC^GCT TCG^CGA AGCGCT | blunt | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. |
| FD0334 | FastDigest Eco52I | BseX3I, BstZI, EagI, EclXI | C^GGCCG GCCGG^C CGGCCG | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD0344 | FastDigest Eco57I | AcuI, Bsp6II, BspD6II, BspKT5I, Eco112I, Eco125I, FsfI, SstE37III | CTGAAGNNNNNNNNNNNNNNNN^ GACTTCNNNNNNNNNNNNNN^NN CTGAAGNNNNNNNNNNNNNNNN | 3’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD0364 | FastDigest Eco72I | AcvI, BbrPI, PmaCI, PmlI, PspCI | CAC^GTG GTG^CAC CACGTG | blunt | 37° C | 80°C, 10 min | Dam: no effect. Dcm: no effect. CpG: blocked. |
| FD0374 | FastDigest Eco81I | AxyI, Bse21I, Bsu36I | CC^TNAGG GGANT^CC CCTNAGG | 5’ protruding ends | 37° C | 80°C, 10 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD0384 | FastDigest Eco88I | Ama87I, AvaI, BmeT110I, BsiHKCI, BsoBIm | C^YCGRG GRGCY^C CYCGRG | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: impaired. EcoKI: no effect. EcoBI: no effect. |
| FD0394 | FastDigest Eco91I | BstEII, BstPI, EcoO65I, PspEI | G^GTNACC CCANTG^G GGTNACC | 5’ protruding ends | 37° C | 65°C, 10 min | Dam: no effect. Dcm: no effect. CpG: no effect. |
| FD0404 | FastDigest Eco105I | BstSNI, SnaBI | TAC^GTA ATG^CAT TACGTA | blunt | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD0414 | FastDigest Eco130I | BssT1I, EcoT14I, ErhI, StyI | C^CWWGG GGWWC^C CCWWGG | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD0424 | FastDigest Eco147I | AatI, PceI, SseBI, StuI | AGG^CCT TCC^GGA AGGCCT | blunt | 37° C | 80°C, 10 min | Dam: no effect. Dcm: blocked if overlapped. CpG: no effect. EcoKI: no effect. |
| FD0434 | FastDigest ScaI | AssI, BmcAI, ZrmI | AGT^ACT TCA^TGA AGTACT | blunt | 37° C | 65°C, 10 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: blocked if overlapped. |
| FD0443 | FastDigest EheI | BbeI, DinI, EgeI, KasI, Mly113I, NarI, SfoI | GGC^GCC CCG^CGG GGCGCC | blunt | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD0444 | FastDigest EheI | BbeI, DinI, EgeI, KasI, Mly113I, NarI, SfoI | GGC^GCC CCG^CGG GGCGCC | blunt | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD0464 | FastDigest GsuI | Bco35I, BpmI, Bsp22I, Bsp28I, BspJ74I, Bth1795I, Uba1437I, Uba1444I | CTGGAGNNNNNNNNNNNNNNNN^ GACCTCNNNNNNNNNNNNNN^NN CTGGAGNNNNNNNNNNNNNNNN | 3’ protruding ends | 30° C | 65°C, 5 min | Dam: no effect. Dcm: blocked if overlapped. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD0474 | FastDigest Hin1I | AcyI, BsaHI, BssNI, BstACI, Hsp92I | GR^CGYC CYGC^RG GRCGYC | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: impaired if overlapped. CpG: blocked. EcoKI: no effect. |
| FD0484 | FastDigest Hin6I | AspLEI, BstHHI, CfoI, HinP1I, HspAI | G^CGC CGC^G GCGC | 5’ protruding ends | 37° C | 80°C, 10 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD0494 | FastDigest HincII | HindII | GTY^RAC CAR^YTG GTYRAC | blunt | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: blocked if overlapped. EcoBI: blocked if overlapped. |
| FD0504 | FastDigest HindIII | A^AGCTT TTCGA^A AAGCTT | 5’ protruding ends | 37° C | 80°C, 10 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: impaired if overlapped. | |
| FD0505 | FastDigest HindIII | A^AGCTT TTCGA^A AAGCTT | 5’ protruding ends | 37° C | 80°C, 10 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. | |
| FD0514 | FastDigest HpaII | BsiSI, HapII | C^CGG GGC^C CCGG | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD0524 | FastDigest KpnI | Asp718I | GGTAC^C C^CATGG GGTACC | 3’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD0534 | FastDigest Kpn2I | AccIII, Aor13HI, BseAI, Bsp13I, BspEI, MroI | T^CCGGA AGGCC^T TCCGGA | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD0544 | FastDigest MspI | BsiSI, HapII | C^CGG GGC^C CCGG | 5’ protruding ends | 37° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD0554 | FastDigest MvaI | AjnI, BseBI, Bst2UI, BstNI, BstOI, Psp6I, PspGI | CC^WGG GGW^CC CCWGG | 5’ protruding ends | 37° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD0564 | FastDigest MluI | A^CGCGT TGCGC^A ACGCGT | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. | |
| FD0573 | FastDigest NcoI | Bsp19I | C^CATGG GGTAC^C CCATGG | 5’ protruding ends | 37° C | 65°C, 15 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD0574 | FastDigest NcoI | Bsp19I | C^CATGG GGTAC^C CCATGG | 5’ protruding ends | 37° C | 65°C, 15 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD0575 | FastDigest NcoI | Bsp19I | C^CATGG GGTAC^C CCATGG | 5’ protruding ends | 37° C | 65°C, 15 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD0583 | FastDigest NdeI | FauNDI | CA^TATG GTAT^AC CATATG | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD0584 | FastDigest NdeI | FauNDI | CA^TATG GTAT^AC CATATG | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD0585 | FastDigest NdeI | FauNDI | CA^TATG GTAT^AC CATATG | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD0593 | FastDigest NotI | CciNI | GC^GGCCGC CGCCGG^CG GCGGCCGC | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD0594 | FastDigest NotI | CciNI | GC^GGCCGC CGCCGG^CG GCGGCCGC | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD0595 | FastDigest NotI | CciNI | GC^GGCCGC CGCCGG^CG GCGGCCGC | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD0596 | FastDigest NotI | CciNI | GC^GGCCGC CGCCGG^CG GCGGCCGC | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD0604 | FastDigest PaeI | BbuI, SphI | GCATG^C C^GTACG GCATGC | 3’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: blocked if overlapped. |
| FD0614 | FastDigest PstI | BspMAI | CTGCA^G G^ACGTC CTGCAG | 3’ protruding ends | 37° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD0615 | FastDigest PstI | BspMAI | CTGCA^G G^ACGTC CTGCAG | 3’ protruding ends | 37° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD0624 | FastDigest PvuI | BpvUI, MvrI, Ple19I | CGAT^CG GC^TAGC CGATCG | 3’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD0634 | FastDigest PvuII | CAG^CTG GTC^GAC CAGCTG | blunt | 37° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. | |
| FD0644 | FastDigest SalI | G^TCGAC CAGCT^G GTCGAC | 5’ protruding ends | 37° C | 65°C, 10 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. | |
| FD0654 | FastDigest SduI | Bsp1286I, MhlI | GDGCH^C C^HCGDG GDGCHC | 3’ protruding ends | 37° C | 80°C, 15 min | Dam: no effect. Dcm: no effect. CpG: no effect. |
| FD0663 | FastDigest SmaI | TspMI, XmaCI, XmaI | CCC^GGG GGG^CCC CCCGGG | blunt | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD0664 | FastDigest SmaI | TspMI, XmaCI, XmaI | CCC^GGG GGG^CCC CCCGGG | blunt | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD0674 | FastDigest TaqI | T^CGA AGC^T TCGA | 5’ protruding ends | 65° C | No (column purification) | Dam: blocked if overlapped. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. | |
| FD0684 | FastDigest XbaI | T^CTAGA AGATC^T TCTAGA | 5’ protruding ends | 37° C | 65°C, 20 min | Dam: blocked if overlapped. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. | |
| FD0685 | FastDigest XbaI | T^CTAGA AGATC^T TCTAGA | 5’ protruding ends | 37° C | 65°C, 20 min | Dam: blocked if overlapped. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. | |
| FD0694 | FastDigest XhoI | PaeR7I, Sfr274I, SlaI, StrI, TliI | C^TCGAG GAGCT^C CTCGAG | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: impaired. EcoKI: no effect. EcoBI: no effect. |
| FD0695 | FastDigest XhoI | PaeR7I, Sfr274I, SlaI, StrI, TliI | C^TCGAG GAGCT^C CTCGAG | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: impaired. EcoKI: no effect. EcoBI: no effect. |
| FD0704 | FastDigest Bst1107I | BssNAI, BstZ17I | GTA^TAC CAT^ATG GTATAC | blunt | 37° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect. |
| FD0714 | FastDigest Van91I | AccB7I, BasI, PflMI | CCANNNN^NTGG GGTN^NNNNACC CCANNNNNTGG | 3’ protruding ends | 37° C | 65°C, 10 min | Dam: no effect. Dcm: blocked if overlapped. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD0724 | FastDigest BclI | FbaI, Ksp22I | T^GATCA ACTAG^T TGATCA | 5’ protruding ends | 37° C | No (column purification) | Dam: blocked. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: blocked if overlapped. |
| FD0734 | FastDigest Mph1103I | EcoT22I, NsiI, Zsp2I | ATGCA^T T^ACGTA ATGCAT | 3’ protruding ends | 37° C | 65°C, 15 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD0744 | FastDigest CpoI | CspI, Rsr2I, RsrII | CG^GWCCG GCCWG^GC CGGWCCG | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD0753 | FastDigest MunI | MfeI | C^AATTG GTTAA^C CAATTG | 5’ protruding ends | 37° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD0754 | FastDigest MunI | MfeI | C^AATTG GTTAA^C CAATTG | 5’ protruding ends | 37° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD0764 | FastDigest Psp5II | PpuMI, PspPPI | RG^GWCCY YCCWG^GR RGGWCCY | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: blocked if overlapped. CpG: no effect. EcoKI: no effect. |
| FD0774 | FastDigest SspI | AAT^ATT TTA^TAA AATATT | blunt | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. | |
| FD0784 | FastDigest Bsp143I | BfuCI, BssMI, BstKTI, BstMBI, DpnII, Kzo9I, NdeII, Sau3AIm | ^GATC CTAG^ GATC | 5’ protruding ends | 37° C | 65°C, 20 min | Dam: no effect. Dcm: no effect. CpG: blocked if overlapped. EcoKI: no effect. EcoBI: no effect. |
| FD0804 | FastDigest HinfI | G^ANTC CTNA^G GANTC | 5’ protruding ends | 37° C | 65°C, 20 min | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: blocked if overlapped. | |
| FD0814 | FastDigest MboI | BfuCI, BssMI, BstKTI, BstMBI, DpnII, Kzo9I, NdeII, Sau3AIm | ^GATC CTAG^ GATC | 5’ protruding ends | 37° C | 65°C, 15 min | Dam: blocked. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: blocked if overlapped. |
| FD0834 | FastDigest BspTI | AflII, BfrI, Bst98I, BstAFI, MspCI, Vha464I | C^TTAAG GAATT^C CTTAAG | 5’ protruding ends | 37° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD0854 | FastDigest Pfl23II | BsiWI, PspLI | C^GTACG GCATG^C CGTACG | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD0884 | FastDigest BseNI | Bse1I, BsrI, BsrSI | ACTGGN^ TGAC^CN ACTGGN | 3’ protruding ends | 65° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. |
| FD0894 | FastDigest Bsh1285I | BsiEI, BstMCI | CGRY^CG GC^YRGC CGRYCG | 3’ protruding ends | 37° C | 80°C, 15 min | Dam: impaired if overlapped. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD0904 | FastDigest Acc65I | Asp718I | G^GTACC CCATG^G GGTACC | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: impaired if overlapped. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect. |
| FD0914 | FastDigest VspI | AseI, PshBI | AT^TAAT TAAT^TA ATTAAT | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD0924 | FastDigest Bsh1236I | AccII, BspFNI, BstFNI, BstUI, MvnI | CG^CG GC^GC CGCG | blunt | 37° C | 80°C, 10 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD0933 | FastDigest Bsp1407I | BsrGI, BstAUI | T^GTACA ACATG^T TGTACA | 5’ protruding ends | 37° C | 80°C, 10 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD0934 | FastDigest Bsp1407I | BsrGI, BstAUI | T^GTACA ACATG^T TGTACA | 5’ protruding ends | 37° C | 80°C, 10 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD0944 | FastDigest Psp1406I | AclI | AA^CGTT TTGC^AA AACGTT | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. |
| FD0973 | FastDigest NheI | AsuNHI, BmtI | G^CTAGC CGATC^G GCTAGC | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect. |
| FD0974 | FastDigest NheI | AsuNHI, BmtI | G^CTAGC CGATC^G GCTAGC | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect. |
| FD0984 | FastDigest Tru1I | MseI, Tru9I | T^TAA AAT^T TTAA | 5’ protruding ends | 65° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: blocked if overlapped. EcoBI: no effect. |
| FD0994 | FastDigest AatII | ZraI | GACGT^C C^TGCAG GACGTC | 3’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD1004 | FastDigest BshNI | AccB1I, BanI, BspT107I | G^GYRCC CCRYG^G GGYRCC | 5’ protruding ends | 37° C | 65°C, 10 min | Dam: no effect. Dcm: impaired if overlapped. CpG: impaired if overlapped. EcoBI: no effect. |
| FD1024 | FastDigest BstXI | CCANNNNN^NTGG GGTN^NNNNNACC CCANNNNNNTGG | 3’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: impaired if overlapped. CpG: no effect. EcoKI: no effect. EcoBI: no effect. | |
| FD1034 | FastDigest KspAI | HpaI | GTT^AAC CAA^TTG GTTAAC | blunt | 37° C | 65°C, 20 min | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: blocked if overlapped. EcoBI: no effect. |
| FD1084 | FastDigest BseDI | BsaJI, BssECI | C^CNNGG GGNNC^C CCNNGG | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD1124 | FastDigest RsaI | AfaI, CviQI, RsaNI | GT^AC CA^TG GTAC | blunt | 37° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect. |
| FD1133 | FastDigest SacI | Eco53kI, EcoICRI, Psp124BI, SstI | GAGCT^C C^TCGAG GAGCTC | 3’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD1134 | FastDigest SacI | Eco53kI, EcoICRI, Psp124BI, SstI | GAGCT^C C^TCGAG GAGCTC | 3’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD1144 | FastDigest TaiI | HpyCH4IV, MaeII | ACGT^ ^TGCA ACGT | 3’ protruding ends | 65° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: blocked. |
| FD1154 | FastDigest BspLI | BmiI, NlaIV, PspN4I | GGN^NCC CCN^NGG GGNNCC | blunt | 37° C | 65°C, 20 min | Dam: no effect. Dcm: impaired if overlapped. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect. |
| FD1164 | FastDigest BfmI | BstSFI, SfcI | C^TRYAG GAYRT^C CTRYAG | 5’ protruding ends | 37° C | 65°C, 20 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD1184 | FastDigest Bpu10I | CC^TNAGC GGANT^CG CCTNAGC | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. | |
| FD1194 | FastDigest SdaI | SbfI, Sse8387I | CCTGCA^GG GG^ACGTCC CCTGCAGG | 3’ protruding ends | 37° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD1204 | FastDigest BseLI | AfiI, Bsc4I, BslI | CCNNNNN^NNGG GGNN^NNNNNCC CCNNNNNNNGG | 3’ protruding ends | 37° C | No (column purification) | Dam: no effect. Dcm: impaired if overlapped. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect. |
| FD1214 | FastDigest MlsI | BalI, MluNI, MscI, Msp20I | TGG^CCA ACC^GGT TGGCCA | blunt | 37° C | 80°C, 20 min | Dam: no effect. Dcm: blocked if overlapped. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD1224 | FastDigest NsbI | Acc16I, AviII, FspI | TGC^GCA ACG^CGT TGCGCA | blunt | 37° C | 65°C, 15 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD1234 | FastDigest AdeI | DraIII | CACNNN^GTG GTG^NNNCAC CACNNNGTG | 3’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. |
| FD1244 | FastDigest SmiI | SwaI | ATTT^AAAT TAAA^TTTA ATTTAAAT | blunt | 37° C | 65°C, 15 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD1253 | FastDigest BcuI | AhlI, SpeI | A^CTAGT TGATC^A ACTAGT | 5’ protruding ends | 37° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. |
| FD1254 | FastDigest BcuI | AhlI, SpeI | A^CTAGT TGATC^A ACTAGT | 5’ protruding ends | 37° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. |
| FD1264 | FastDigest BseMI | Bse3DI, BsrDI | GCAATGNN^ CGTTAC^NN GCAATGNN | 3’ protruding ends | 55° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD1274 | FastDigest MbiI | AccBSI, BsrBI | CCG^CTC GGC^GAG CCGCTC | blunt | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: impaired. EcoKI: no effect. |
| FD1284 | FastDigest PagI | CciI, BspHI, RcaI | T^CATGA AGTAC^T TCATGA | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: impaired if overlapped. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: impaired if overlapped. |
| FD1294 | FastDigest TatI | W^GTACW WCATG^W WGTACW | 5’ protruding ends | 65° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. | |
| FD1304 | FastDigest XagI | BstENI, EcoNI | CCTNN^NNNAGG GGANNN^NNTCC CCTNNNNNAGG | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD1314 | FastDigest BplI | GAAGACNN^NNNN CTTCTGNNNNNN^ GAAGACNNNNNN | 3’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. | |
| FD1334 | FastDigest PsyI | AspI, PflFI, Tth111I | GACN^NNGTC CTGNN^NCAG GACNNNGTC | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD1344 | FastDigest MssI | PmeI | GTTT^AAAC CAAA^TTTG GTTT^AAAC | blunt | 37° C | 65°C, 10 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: blocked if overlapped. EcoBI: no effect. |
| FD1354 | FastDigest TasI | Sse9I, Tsp509I, TspEI | ^AATT TTAA^> AATT | 5’ protruding ends | 65° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: blocked if overlapped. |
| FD1364 | FastDigest TaaI | Bst4CI, HpyCH4III | ACN^GT TG^NCA ACNGT | 3’ protruding ends | 65° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. |
| FD1374 | FastDigest SchI | MlyI, PleI, PpsI, SchI | GAGTCNNNNN^ CTCAGNNNNN GAGTCNNNNN | blunt | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD1383 | FastDigest XapI | AcsI, ApoI | R^AATTY YTTAA^R RAATTY | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD1384 | FastDigest XapI | AcsI, ApoI | R^AATTY YTTAA^R RAATTY | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD1394 | FastDigest CaiI | AlwNI | CAGNNN^CTG GTC^NNNGAC CAGNNNCTG | 3’ protruding ends | 37° C | 65°C, 10 min | Dam: no effect. Dcm: blocked if overlapped. CpG: no effect. EcoKI: no effect. |
| FD1404 | FastDigest BseMII | BspCNI | GCAATGNN^ CGTTAC^NN GCAATGNN | 3’ protruding ends | 55° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD1414 | FastDigest ApaI | PspOMI | GGGCC^C C^CCGGG GGGCCC | 3’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: impaired if overlapped. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect. |
| FD1424 | FastDigest Bme1390I | BmrFI, BssKI, BstSCI, MspR9I, ScrFI, StyD4I | CC^NGG GGN^CC CCNGG | 5’ protruding ends | 37° C | No (column purification) | Dam: no effect. Dcm: blocked if overlapped. CpG: blocked if overlapped. EcoKI: no effect. EcoBI: no effect. |
| FD1434 | FastDigest BoxI | BstPAI, PshAI | GACNN^NNGTC CTGNN^NNCAG GACNNNNGTC | blunt | 37° C | 80°C, 20 min | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. |
| FD1444 | FastDigest BseSI | BaeGI, BstSLI | GKGCM^C C^MCGKG GKGCMC | 3’ protruding ends | 37° C | 80°C, 15 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: impaired if overlapped. EcoBI: no effect. |
| FD1454 | FastDigest BseXI | BbvI, BstV1I | GCAGCNNNNNNNN^NNNN CGTCGNNNNNNNNNNNN^ GCAGCNNNNNNNNNNNN | 5’ protruding ends | 65° C | 80°C, 15 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD1464 | FastDigest BshTI | AgeI, AsiGI, CspAI, PinAI | A^CCGGT TGGCC^A ACCGGT | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. |
| FD1474 | FastDigest XceI | BstNSI, NspI | RCATG^Y Y^GTACR RCATGY | 3’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD1484 | FastDigest XmiI | AccI, FblI | GT^MKAC CAKM^TG GTMKAC | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked if overlapped. EcoKI: no effect. EcoBI: no effect. |
| FD1514 | FastDigest NmuCI | Tsp45I | ^GTSAC CASTG^ GTSAC | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. |
| FD1524 | FastDigest PdiI | MroNI, NaeI, NgoMIV | GCC^GGC CGG^CCG GCCGGC | blunt | 37° C | 65°C, 15 min | Dam: no effect. Dcm: no effect. CpG: blocked if overlapped. EcoKI: no effect. EcoBI: no effect. |
| FD1534 | FastDigest PdmI | Asp700I, MroXI, XmnI | GAANN^NNTTC CTTNN^NNAAG GAANNNNTTC | blunt | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. |
| FD1554 | FastDigest PsuI | BstX2I, BstYI, MflI, XhoII | R^GATCY YCTAG^R RGATCY | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD1564 | FastDigest XmaJI | AspA2I, AvrII, BlnI | C^CTAGG GGATC^C CCTAGG | 5’ protruding ends | 37° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD1574 | FastDigest Hpy8I | Hpy166II | GTN^NAC CAN^NTG GTNNAC | blunt | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. |
| FD1634 | FastDigest OliI | AleI | CACNN^NNGTG GTGNN^NNCAC CACNNNNGTG | blunt | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: blocked if overlapped. EcoBI: blocked if overlapped. |
| FD1644 | FastDigest SatI | Fnu4HI, Fsp4HI, ItaI | GC^NGC CGN^CG GCNGC | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked if overlapped. EcoKI: no effect. EcoBI: no effect. |
| FD1654 | FastDigest TauI | GCSG^C C^GSCG GCSGC | 3’ protruding ends | 55° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. | |
| FD1664 | FastDigest FspAI | RTGC^GCAY YACG^CGTR RTGCGCAY | blunt | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. | |
| FD1703 | FastDigest DpnI | MalI | GA^TC CT^AG GATC | blunt | 37° C | 80°C, 5 min | Dam: does not cut dam- DNA. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD1704 | FastDigest DpnI | MalI | GA^TC CT^AG GATC | blunt | 37° C | 80°C, 5 min | Dam: does not cut dam - DNA. Dcm: no effect. Cpg: no effect. EcoKI: no effect. |
| FD1714 | FastDigest BseJI | BsaBI, Bse8I | GATNN^NNATC CTANN^NNTAG GATNNNNATC | blunt | 37° C | No (column purification) | Dam: blocked if overlapped. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD1724 | FastDigest AasI | DrdI, DseDI | GACNNNN^NNGTC CTGNN^NNNNCAG GACNNNNNNGTC | 3’ protruding ends | 37° C | 80°C, 10 min | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect. |
| FD1734 | FastDigest HpyF10VI | BstMWI, MwoI | GCNNNNN^NNGC CGNN^NNNNNCG GCNNNNNNNGC | 3’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect. |
| FD1744 | FastDigest BveI | Acc36I, BfuAI, BspMI | ACCTGCNNNN^NNNN TGGACGNNNNNNNN^ ACCTGCNNNNNNNN | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. |
| FD1754 | FastDigest PfoI | T^CCNGGA AGGNCC^T TCCNGGA | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: impaired if overlapped. Dcm: blocked if overlapped. CpG: blocked if overlapped. EcoKI: no effect. EcoBI: no effect. | |
| FD1764 | FastDigest FspBI | BfaI, MaeI, XspI | C^TAG GAT^C CTAG | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD1784 | FastDigest PfeI | TfiI | G^AWTC CTWA^G GAWTC | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked if overlapped. EcoKI: no effect. |
| FD1794 | FastDigest SsiI | AciI, BspACI | C^CGC GGC^G CCGC | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD1814 | FastDigest FaqI | BslFI, BsmFI | GGGACNNNNNNNNNN^NNNN CCCTGNNNNNNNNNNNNNN^ GGGACNNNNNNNNNNNNNN | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked if overlapped. EcoKI: no effect. EcoBI: no effect. |
| FD1824 | FastDigest SfiI | GGCCNNNN^NGGCC CCGGN^NNNNCCGG GGCCNNNNNGGCC | 3’ protruding ends | 50° C | No (column purification) | Dam: no effect. Dcm: impaired if overlapped. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect. | |
| FD1834 | FastDigest Hin1II | CviAII, FaeI, FatI, Hsp92II, NlaIII | CATG^ ^GTAC CATG | 3’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD1854 | FastDigest HhaI | AspLEI, BstHHI, CfoI, HinP1I, HspAI | GCG^C C^GCG GCGC | 3’ protruding ends | 37° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD1884 | FastDigest HpyF3I | BstDEI, DdeI | C^TNAG GANT^C CTNAG | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD1894 | FastDigest SgsI | AscI, PalAI | GG^CGCGCC CCGCGC^GG GGCGCGCC | 5’ protruding ends | 37° C | 65°C, 20 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD1904 | FastDigest CseI | HgaI | GACGCNNNNN^NNNNN CTGCGNNNNNNNNNN^ GACGCNNNNNNNNNN | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. |
| FD1954 | FastDigest AjuI | NNNNN^NNNNNNNGAANNNNNNNTTGGNNNNNNNNNNN^ ^NNNNNNNNNNNNCTTNNNNNNNAACCNNNNNN^NNNNN NNNNNNNNNNNNGAANNNNNNNTTGGNNNNNNNNNNN | 3’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. | |
| FD1974 | FastDigest Ppu21I | BsaAI, BstBAI | YAC^GTR RTG^CAY YACGTR | blunt | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked if overlapped. |
| FD2004 | FastDigest RseI | MslI, SmiMI | CAYNN^NNRTG GTRNN^NNYAC CAYNNNNRTG | blunt | 37° C | 65°C, 20 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: blocked if overlapped. |
| FD2024 | FastDigest MreI | CG^CCGGCG GCGGCC^GC CGCCGGCG | 3’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. | |
| FD2044 | FastDigest BspOI | AsuNHI, BmtI | GCTAG^C C^GATCG GCTAGC | 3’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD2064 | FastDigest AanI | PsiI | TTA^TAA AAT^ATT TTATAA | blunt | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoBI: no effect. |
| FD2074 | FastDigest Lsp1109I | BbvI, BstV1I | GCAGCNNNNNNNN^NNNN CGTCGNNNNNNNNNNNN^ GCAGCNNNNNNNNNNNN | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD2084 | FastDigest MauBI | CG^CGCGCG GCGCGC^GC CGCGCGCG | 3’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. | |
| FD2094 | FastDigest SfaAI | AsiSI, RgaI, SgfI | GCGAT^CGC CGC^TAGCG GCGATCGC | 3’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD2104 | FastDigest TscAI | TspRI | NNCASTGNN^ ^NNGTSACNN NNCASTGNN | 3’ protruding ends | 65° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. |
| FD2114 | FastDigest CsiI | MabI, SexAI | A^CCWGGT TGGWCC^A ACCWGGT | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. |
| FD2134 | FastDigest PteI | BsePIm BssHII | G^CGCGC CGCGC^G GCGCGC | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD2144 | FastDigest FokI | BstF5I, BtsCI, FokI | GGATGNNNNNNNNN^NNNN CCTACNNNNNNNNNNNNN^ GGATGNNNNNNNNNNNNN | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD2154 | FastDigest RruI | BtuMI, NruI | TCG^CGA AGC^GCT TCGCGA | blunt | 37° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD2164 | FastDigest KflI | Sse1825I, SanDI | GG^GWCCC CCCWG^GG GGGWCCC | 5’ protruding ends | 37° C | No (column purification) | Dam: no effect. CpG: blocked if overlapped. EcoKI: no effect. EcoBI: no effect. |
| FD2174 | FastDigest SaqAI | MseI, Tru1I, Tru9I | T^TAA AAT^T TTAA | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoBI: no effect. |
| FD2184 | FastDigest BfoI | AccB2I, Bme142I, BsmHI, Bsp143II, Bst16I, Bst1473II, BstH2I, Btu34II, HaeII, HinHI, LpnI, NgoAI, NgoBI, NgoCI, NgoGI, NgoJI, NgoMI, NgoWI | RGCGC^Y Y^CGCGR RGCGCY | 3’ protruding ends | 37° C | 65°C, 10 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. |
| FD2204 | FastDigest PacI | TTAAT^TAA AAT^TAATT TTAATTAA | 3’ protruding ends | 37° C | 65°C, 10 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoBI: no effect. | |
| FD2224 | FastDigest PspFI | CCCAG^C G^GGTCG CCCAGC | 3’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect. |
仅供科研使用，不可用于诊断目的。