Tundra Cryo TEM Features

The Thermo Scientific Tundra Cryo-Transmission Electron Microscope (Cryo-TEM) allows you to perform structural analyses on challenging proteins and macromolecules with unprecedented ease of use, at a price point within the reach of many grants and funding sources.

Tundra Cryo-TEM benefits

Shorten time to cryo-EM sample selection

 

The Tundra Cryo-TEM can visualize the impact of biochemical adjustments to samples faster than other technologies. Exchange samples in and out of the microscope in minutes, with nearly instantaneous feedback, allowing you to optimize your sample conditions quickly without extensive training.

An example of cryo-EM sample behaving differently in ice. Sample courtesy of Guillaume Lebon, Université Montpellier, CNRS, INSERM.

Structural information at biologically relevant resolution

 

The optics of the Tundra Cryo-TEM are specifically designed so that you can answer fundamental questions on biology and human disease with data at biologically relevant resolutions. Operating at 100 kV, the Tundra Cryo-TEM can generate high-contrast biological images routinely at near atomic resolution with medium throughput with the high performance Thermo Scientific Falcon C Direct Electron Detector. 

Structure of T20S Proteasome at 2.7 angstroms determined on a Tundra Cryo-TEM.

Designed to meet your budget

A wide range of funding proposals and grants were considered when designing the Tundra Cryo-TEM to keep its price within the reach of most instrumentation grants. Our Electron Microscopy Funding Support Center has additional information about our financial services as well as grant-writing guidance so you can get the tools you need, when you need them.

Advanced cryo-TEM software takes the guesswork out of cryo-TEM

The Tundra Cryo-TEM comes with a complete suite of automation software for efficient optimization of your sample’s biochemistry as well as data collection for structural determination. This includes a user-friendly AI-enabled software solution capable of analyzing intermediate results, providing instant feedback, and steering data collection on the fly. Our AI algorithms are based on years of cryo-EM knowledge, providing expert decisions upfront to ensure that your instrument is working at optimal conditions. This allows you to focus on the science rather than on fine-tuning the microscope.

Tundra Cryo-TEM features

The Tundra Cryo-TEM is equipped with a 100 kV high-brightness field emission gun (X-FEG) that allows for high resolution structure determination at routinely 3-5 Å. The Tundra Cryo-TEM can be installed with either the Falcon C Direct Electron Detector or Thermo Scientific Ceta F CMOS Detector. The Falcon C Direct Electron Detector provides a boost in contrast for single particle cryo-EM, improving resolution and obtaining results faster with less data to maximize your resolution efforts. Tundra Cryo-TEM with the Falcon C Direct Electron Detector makes it possible to efficiently solve protein structures 100 kDa or less.

Tundra Cryo-TEM loading station

An integrated loader makes it easier for users of any experience level to load samples into the microscope, as compared to conventional systems. Scientists can exchange sample carriers in minutes. Results can be checked immediately, allowing users to rapidly optimize biochemistry sample conditions.

 

The cryo-loading station stays cold for approximately 6.5 hours. With a 20-minute loading and unloading cycle, as well as time for sample quality analysis, users can exchange up to 10 sample grids a day. 

Tundra Cryo-TEM technical specifications

Source
  • X-FEG (extreme high-brightness field emission gun)

Accelerating voltage
  • Fixed accelerating voltage of 100 kV

Semi-automated sample loading
  • Cryo-preparation station for contamination free sample exchange
  • Sample transfer device for transfer of single AutoGrids to the microscope, with fixed cryo-box

Lenses
  • High-resolution objective lens optimized for single particle analysis

Stage
  • Computerized specimen cryo-stage

Imaging options
  • High-performance Falcon C Direct Electron Detector for improved resolution capabilities
  • Standard Ceta-F CMOS Camera with dose fractionation, optimized for low-dose imaging

Room size requirements (L x W X H)
  • All in one room dimensions: 4.20 x 4.00 x 2.74 m (13.8 x 13.1 x 8.99 ft)
  • Dimensions with cryo-loading station in neighboring room: 4.00 x 3.80 x 2.74 m (13.1 x 12.5 x 8.99 ft)

Software
  • AI-enabled and automated software solution that provides feedback during data collection and has an open application programming interface

Tundra Cryo-TEM floor-plan and installation requirements

  • Environmental temperature: 18–23°C 
  • Temperature stability: 1°C per 24 hours 
  • Relative humidity: <60%
  • All-in-one room dimensions: 4.20 x 4.00 m (13.8 x 13.1 ft)
  • Dimensions with cryo-loading station in neighboring room: 4.00 x 3.80 m (13.1 x 12.5 ft) 
  • Ceiling height: 2.74 m (8.99 ft)
  • Door height: 2.30 m (7.55 ft); can be optionally reduced to 1.97 m (6.5 ft)
  • Door width: 1.00 m (3.28 ft)
  • Weight distribution maximum: 700 kg/m2
  • Double earth connection
  • Frequency: 50 or 60 Hz (±3%)
  • Compressed air supply with a pressure range of 5–7 bar
  • Sulfur hexafluoride (SF6) gas in properly ventilated room
  • LAN connection for Thermo Scientific RAPID Service (Remote Access Program for Interactive Diagnosis)

For Research Use Only. Not for use in diagnostic procedures.