Custom Guinea Pig Polyclonal Antibody Production Protocols

Immunization and serum-collection procedures for custom guinea pig polyclonal antibody production

Our array of custom antibody services includes a customizable option for guinea pig immunization, serum collection, and titer analysis for guinea pig antibody production, plus several extension possibilities.

Custom Antibody Request

Highlights

Custom guinea pig antibodies—a reliable protocol for immunizing guinea pigs, collecting serum, testing bleeds, reporting titer results, and delivering antisera
Full-service integration—combine this immunization service with our complete set of services for antigen preparation and antibody purification

2-guinea pig 80-day immunization protocol

This guinea pig immunization schedule uses two Hartley Pigmented guinea pigs for each immunogen. Animals are housed individually in separate cages. Injections are subcutaneous (SQ) as emulsions in Complete Freund's Adjuvant (CFA) or Incomplete Freund's Adjuvant (IFA); alternative adjuvants can be used upon request. Customizations to the protocol are available. For example, you can choose the number of guinea pigs needed and you can adjust the length of your protocol beyond the 80-day immunization schedule to meet your research goals. Please contact us to discuss the options in detail. Antigen preparation, such as peptide synthesis and/or immunogen conjugation, occurs before Day 0. Protocol Days are approximate (± 2 days).

Procedure	Protocol day	Description
Control serum collection	Day 0	Pre-immune bleed (~1 mL per guinea pig)
Primary injection	Day 1	Immunize with 100 µg antigen in CFA, SQ 4 sites
1st booster	Day 21	Boost with 50 µg antigen in IFA, SQ 4 sites
2nd booster	Day 42	Boost with 50 µg antigen in IFA, SQ 4 sites
Test bleed	Day 50	Test-bleed (~1 mL per guinea pig)
ELISA and shipping	Day 60	ELISA titration of pre-immune and test bleed; (results available online)
3rd booster	Day 62	Boost with 50 µg antigen in IFA, SQ 4 sites
Decision	Day 64	Verify disposition of animals; decide to continue or terminate
Instruction due date	Day 80	Terminal bleed (exsanguination, yielding 10–15 mL per guinea pig) and termination of animals if instructions not received
Deliverables: 8 vials crude antibody sera 2 vials: 1 mL (1 per animal) test aliquots pre-immune sera 2 vials: 1 mL (1 per animal) test bleeds post-inject sera (Day 50) 2 vials: 10–15 mL terminal bleed (1 per animal; exsanguination bleed) 2 vials: 1 mL test bleeds post-inject sera (1 per animal; exsanguination bleed)