人类 T 细胞活化和扩增

用于人类 T 细胞扩增的 Dynabeads

在转化研究中，即用型 Dynabeads 能够在保持 T 细胞的活性和较佳免疫功能的同时实现体外 T 细胞活化和扩增的优化。对 TCR/CD3 和 CD28 同时发出信号可触发人类 T 细胞的生理活化和扩增。您还可使用 Dynabeads 人类 T 细胞活化剂（T-Activator）CD3/CD28/CD137 刺激和扩增人类抗原特异性 T 细胞系或克隆。

这种简单、高效且一致的技术优于并替代了传统的基于抗原呈递细胞 (APC)、有丝分裂原（如 ConA、PHA）、可溶性/板结合抗体或化学活化剂的自制通用活化方法，已发表的文献对此进行了详细记录。基因工程改造 T 细胞的扩增和回输有望彻底改变白血病和相关血液癌症的治疗方法。我们还提供临床研究级版本，以便您可以使用相同的技术平台从小鼠研究转向临床研究。
 

人类 T 细胞活化和扩增选择指南

以下列出的参考文献是众多已发表论文中的一部分，这些论文记录了 Dynabeads 技术在 T 细胞活化和扩增方面的表现：

1. Eyquem, J et al.(2017) Targeting a CAR to the TRAC locus with CRISPR/Cas9 enhances tumour rejection.Nature 543: 113-117
2. Mock, U et al.(2016) Automated manufacturing of chimeric antigen receptor T cells for adoptive immunotherapy using CliniMACS prodigy.Cytotherapy 18: 1002-1011
3. Suarez, ER et al.(2016) Chimeric antigen receptor T cells secreting anti-PD-L1 antibodies more effectively regress renal cell carcinoma in a humanized mouse model.Oncotarget 7: 34341-34355
4. Jacomet, F et al.(2015) Evidence for eomesodermin‐expressing innate‐like CD8⁺ KIR/NKG2A⁺ T cells in human adults and cord blood samples.Eur J Immunol. 45: 1926-33
5. Kane, M et al.(2013) MX2 is an interferon-induced inhibitor of HIV-1 infection.Nature 502: 563-566
6. Levring, TB et al.(2012) Activated human CD4⁺ T cells express transporters for both cysteine and cystine.Sci Rep.2：266
7. Porter, DL et al.(2011) Chimeric Antigen Receptor–Modified T Cells in Chronic Lymphoid Leukemia.N Engl J Med 365:725-733.
8. Kalos M et al (2011) T Cells with Chimeric Antigen Receptors Have Potent Antitumor Effects and Can Establish Memory in Patients with Advanced Leukemia. Sci Transl Med 3:95ra73 
9. Bonyhadi M et al (2005) In vitro engagement of CD3 and CD28 corrects T cell defects in chronic lymphocytic leukemia.J Immunol 174:2366–2375.
10. Rapoport AP et al (2005) Restoration of immunity in lymphopenic individuals with cancer by vaccination and adoptive T-cell transfer.Nat Med 11:1162–1163.
11. Godfrey WR et al (2004) In vitro expanded human CD4+CD25+ T regulatory cells can markedly inhibit allogeneic dendritic cell stimulated MLR cultures.Blood 104:453–461.
12. Coito S et al.(2004) Retrovirus-mediated gene transfer in human primary T lymphocytes induces an activation-and transduction/selection-dependent TCRBV repertoire skewing of gene-modified cells.Stem Cells Dev 13:71–81.
13. Berger C et al (2003) CD28 costimulation and immunoaffinity-based selection efficiently generate primary gene-modified T cells for adoptive immunotherapy.Blood 101:476–484.
14. Levine BL et al.(2002) Adoptive transfer of costimulated CD4+ T cells induces expansion of peripheral T cells and decreases CCR5 expression in HIV infection.Nat Med 8:47–53.