• 首款用于免疫学研究的成分完全明确的市售无血清培养基
  • 不同批次间产品质量稳定，生产过程符合 cGMP 标准规范

性能优越，无需血清

Gibco AIM V 培养基无需人血清，即可达到分化型淋巴样细胞的最佳生长和活化条件。

  • 将人血清相关的病毒免疫原性风险降至最低
  • 未添加细胞因子和淋巴因子
  • 配方中的所有蛋白质均与人体蛋白质序列相同
     

正在将研究转向临床？请务必了解一下 CTS AIM V 培养基

AIM V 培养基与含人血清培养基的比较

分别使用 AIM V 培养基和含血清的 RPMI-1640 培养基培养淋巴细胞，并对它们的相对 LAK 细胞裂解活性进行对比，结果如图1所示。

      图 1. LAK 细胞裂解活性对比：AIM V 培养基与含人血清培养基的比较。使用含 1,000 U/mL IL-2 和 2% AB 型人血清的 RPMI-1640 培养人外周血淋巴细胞 (PBL)。同时，使用含 600 U/mL IL-2 的无血清 AIM V 培养基培养人 PBL。以两个相同培养瓶中的细胞进行三次重复实验并取平均值，靶标为 Daudi 细胞。

AIM V 培养基的应用

AIM V 培养基经过测试，可广泛应用于过继免疫治疗和细胞免疫学研究。下表列出了一些应用：

LAK 细胞诱导和功能细胞因子和抗体对 LAK 细胞诱导的影响
小鼠肝转移癌的免疫治疗淋巴细胞受体研究
中空纤维生物反应器中的 TIL 细胞生成NK 细胞和 NK 样效应细胞群
T 细胞活化过继免疫治疗的体外和体内模型系统
效应细胞的细胞因子调控HIV 研究
淋巴样效应细胞的细胞因子受体及受体后事件巨噬细胞活化
杂交瘤细胞生长和单克隆抗体生产抗体依赖性细胞毒性
抗肿瘤免疫中的效应细胞功能性人巨噬细胞的长期培养
淋巴因子和抗体的体外研究细胞介导的细胞毒性反应机制

使用 AIM V 培养基培养的细胞类型

T 细胞类型

  • CD8+ T 淋巴细胞
  • 多克隆 T 细胞
  • 外周血淋巴细胞 (PBL)
  • HIV 患者的 CD4 细胞
  • 肿瘤浸润淋巴细胞 (TIL)
  • 逆转录病毒转导的 PBL
  • CD3+ NK T 细胞
  • 抗原特异性细胞（非克隆）
  • γδ T 细胞
  • NK 细胞

其他细胞类型

  • 巨噬细胞
  • 人单核细胞
  • HUT78（人）
  • 树突状细胞
  • HL-60
  • COS 细胞（猴）
  • YAC-1（小鼠）
  • 鼠淋巴瘤细胞
  • 骨髓细胞
  • 白血病细胞
  • 人成纤维细胞
  • VERO 细胞
  • 口腔上皮细胞
AIM-V 培养基的主要参考文献
  1. Dietze B., et al.(2008) An improved method to generate equine dendritic cells from peripheral blood mononuclear cells: divergent maturation programs by IL-4 and LPS.Immunobiology 213:751–758.
  2. Meehan K.R., et al.(2008) Development of a clinical model for ex vivo expansion of multiple populations of effector cells for adoptive cellular therapy.Cytotherapy 10: 30–37.
  3. Ye Z., et al.(2006) Human dendritic cells engineered to express alpha tumor necrosis factor maintain cellular maturation and T-cell stimulation capacity.Cancer Biother Radiopharm 21:613–622.
  4. Choi B.H., et al.(2006) Optimization of the concentration of autologous serum for generation of leukemic dendritic cells from acute myeloid leukemic cells for clinical immunotherapy.J Clin Apher 21: 233–240.
  5. Imataki O., et al.(2006) Efficient ex vivo expansion of Valpha24+ NKT cells derived from G-CSF-mobilized blood cells.J Immunother 29: 320–327. 
  6. Peng J.C., et al.(2005) Generation and maturation of dendritic cells for clinical application under serum-free conditions.J Immunother 28: 599–609.
  7. Trickett A.E., et al.(2002) Ex vivo expansion of functional T lymphocytes from HIV-infected individuals.J Immunol Methods 262: 71–83.
  8. Carlens S., et al.(2000) Ex vivo T lymphocyte expansion for retroviral transduction: influence of serum-free media on variations in cell expansion rates and lymphocyte subset distribution.Exp Hematol 28: 1137–1146.
  9. Kambe N et al.(2000) An improved procedure for the development of human mast cells from dispersed fetal liver cells in serum-free culture medium.J Immunol Methods 240: 101–110.
  10. Gerin P.A., et al.(1999) Production of retroviral vectors for gene therapy with the human packaging cell line FLYRD18.Biotechnol Prog 15: 941–948. 
  11. Slunt J.B., et al.(1997) Human T-cell responses to Trichophyton tonsurans: inhibition using the serum free medium Aim V. Clin Exp Allergy 27: 1184–1192.
  12. Kreuzfelder E., (1996) Assessment of peripheral blood mononuclear cell proliferation by [2-3H]adenine uptake in the woodchuck model.Clin Immunol Immunopathol 78: 223–227.
  13. Causey A.L., (1994) A serum-free medium for human primary T lymphocyte culture.J Immunol Methods 175: 115–121.
  14. Freedman R.S., et al.(1994) Large-scale expansion in interleukin-2 of tumor-infiltrating lymphocytes from patients with ovarian carcinoma for adoptive immunotherapy.J Immunol Methods 167: 145–160.
  15. Nomura K., et al.(1993) [Study of adoptive immunotherapy for metastatic renal cell carcinoma with lymphokine-activated killer (LAK) cells and interleukin-2.II.Clinical evaluation] Nippon Hinyokika Gakkai Zasshi 84: 831–840.Japanese.
  16. Kaldjian E.P., et al.(1992) Enhancement of lymphocyte proliferation assays by use of serum-free medium.J Immunol Methods 147: 189–195.
  17. Hayakawa K., et al.(1991) Study of tumor-infiltrating lymphocytes for adoptive therapy of renal cell carcinoma (RCC) and metastatic melanoma: sequential proliferation of cytotoxic natural killer and noncytotoxic T cells in RCC.J Immunother 10: 313–325.
  18. McVicar D.W., et al.(1991) A comparison of serum-free media for the support of in vitro mitogen-induced blastogenic expansion of cytolytic lymphocytes.Cytotechnology 6: 105–113.
  19. Burg S., et al.(1991) [Effect of different media on long-term cultivation of human synovial macrophages] Z Rheumatol 50: 142–150.German.
  20. Helinski E.H., et al.(1988) Long-term cultivation of functional human macrophages in Teflon dishes with serum-free media.J Leukoc Biol 44: 111–121.
  21. Robyn S., et al.(2007) RA8, A Human Anti-CD25 Antibody Against Human Treg Cells Hybridoma 26: 119–130.
  22. Chena X., et al.(2006) Induction of primary anti-HIV CD4 and CD8 T cell responses by dendritic cells transduced with self-inactivating lentiviral vectors Cellular Immunology 243: 10–18.
  23. Grant R., et al.(2008) CCL2 Increases X4-tropic HIV-1 Entry into Resting CD4+ T Cells.J Biol Chem 283: 30745–30753.
  24. Hagihara M., et al.(2003) Increased frequency of CD3/8/56-positive umbilical cord blood T lymphocytes after allo-priming in vitro Annals of Hematology 82: 166–170.
  25. Wang, Z., et al.(2006) Application of Serum-Free Culture Medium for Preparation of A-NK Cells Cell Mol Immunol 3: 391–395.
  26. Morecki S., et al.(1991) Retrovirus-mediated gene transfer into CD4+ and CD8+ human T cell subsets derived from tumor-infiltrating lymphocytes and peripheral blood mononuclear cells Cancer Immunol Immunotherapy 32: 342–352.
  27. Johansen P. et al.Cellular immune response—CD4 T cells guarantee optimal competitive fitness of CD8 memory T cells Eur J Immunol 34: 91–97.

其他 AIM V 培养基参考文献

  1. Rebecca J et al., (2010) Natural exposure to cutaneous anthrax gives long lasting T cell immunity encompassing infection-specific Epitopes.J. Immunol.,  2010; 184: 3814 – 3821
  2. Fabricius D et al., (2010) Prostaglandin E2 inhibits IFN-α secretion and Th1 costimulation by human plasmacytoid dendritic cells via E-prostanoid 2 and E-prostanoid 4 receptor engagement.J. Immunol., 2010; 184: 677 - 684.
  3. Jahrsdorfer B et al., Granzyme B produced by human plasmacytoid dendritic cells suppresses T-cell expansion.Blood, 2010; 115: 1156 - 1165.
  4. Tam V et al., The RgpA-Kgp Proteinase-Adhesin Complexes of Porphyromonas gingivalis Inactivate the Th2 Cytokines Interleukin-4 and Interleukin-5.Infect.Immun., 2009; 77: 1451 - 1458.
  5. Ando T et al., Transduction with the Antioxidant Enzyme Catalase Protects Human T Cells against Oxidative Stress J. Immunol., 2008; 181: 8382 - 8390.
  6. Nesbit L et al., Polyfunctional T Lymphocytes Are in the Peripheral Blood of Donors Naturally Immune to Coccidioidomycosis and Are Not Induced by Dendritic Cells.Infect.Immun., 2010; 78: 309 - 315.
  7. Csillag A et al., Pollen-Induced Oxidative Stress Influences Both Innate and Adaptive Immune Responses via Altering Dendritic Cell Functions.J. Immunol., 2010; 184: 2377 - 2385.
  8. Bellone S et al., Human Papillomavirus Type 16 (HPV-16) Virus-Like Particle L1-Specific CD8+ Cytotoxic T Lymphocytes (CTLs) Are Equally Effective as E7-Specific CD8+ CTLs in Killing Autologous HPV-16-Positive Tumor Cells in Cervical Cancer Patients: Implications for L1 Dendritic Cell-Based Therapeutic Vaccines.J. Virol., 2009; 83: 6779 - 6789.
  9. Liu ZW et al., A CD26-Controlled Cell Surface Cascade for Regulation of T Cell Motility and Chemokine Signals.J. Immunol., 2009; 183: 3616 - 3624.
  10. Megyeri M et al., Complement Protease MASP-1 Activates Human Endothelial Cells: PAR4 Activation Is a Link between Complement and Endothelial Function.J. Immunol., 2009; 183: 3409 - 3416.
  11. Asish K. et al., Curcumin Inhibits Prosurvival Pathways in Chronic Lymphocytic Leukemia B Cells and May Overcome Their Stromal Protection in Combination with EGCG.Clin.Cancer Res., 2009; 15: 1250 -1258
  12. Cornberg M et al., CD8 T Cell Cross-Reactivity Networks Mediate Heterologous Immunity in Human EBV and Murine Vaccinia Virus Infections.J. Immunol., 2010; 184: 2825 - 2838.
  13. Ariadne L et al., The Gli3 Transcription Factor Expressed in the Thymus Stroma Controls Thymocyte Negative Selection Via Hedgehog-Dependent and -Independent Mechanisms.J. Immunol., 2009; 183: 3023 - 3032.
  14. Hagn M et al., Human B Cells Secrete Granzyme B When Recognizing Viral Antigens in the Context of the Acute Phase Cytokine IL-21.J. Immunol., 2009; 183: 1838 - 1845.
  15. Lenka L et al., Apolipoprotein-mediated lipid antigen presentation in B cells provides a pathway for innate help by NKT cells.Blood, 2009; 114: 2411 - 2416.
  16. Toll-like Receptor-7 Tolerizes Malignant B Cells and Enhances Killing by Cytotoxic Agents.Cancer Res., 2007; 67: 1823 - 1831.
  17. Andrei V et al.,  Inhibition of glycogen synthase kinase-3 activity leads to epigenetic silencing of nuclear factor  B target genes and induction of apoptosis in chronic lymphocytic leukemia B cells.Blood, 2007; 110: 735 - 742.
  18. Caitlin M et al.,   Extracellular calcium sensing promotes human B-cell activation and function.Blood, 2007; 110: 3985 - 3995.

AIM V 培养基广泛应用于 T 细胞、T 细胞系、淋巴结细胞和树突状细胞。

  • 据报道，HUVEC 细胞在含 1% FBS 和生长因子的 AIM V 中培养（参考文献10）
  • CLL B 细胞（参考文献11、17）
  • CLL 细胞（参考文献16）
  • 胎儿胸腺（参考文献13）
  • 人 B 细胞（参考文献14、15、18）