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One of the most difficult viruses to extract is HBV due to the rigid protein coat. Sensitive detection requires a strong lysis and pure DNA. To look at the ability of the PureLink Viral RNA/DNA Mini Kit to efficiently extract HBV virus, samples of infectious patient plasma were extracted side by side with plasma spiked with ZeptoMetrix's HBV nucliec acid test control (NATtrol™). HBV was detected in plasma samples diluted 1:100 from both patients (grey bars) and in NATtrol™ spiked samples (blue bars) (Figure 1).
图1.Equivalent Recovery of HBV in Patient Samples and Plasma Spiked with NATtrol™ Nucleic Acid Test Controls by ZeptoMetrix using PureLink Viral RNA/DNA Mini Kit. HBV was extracted from infectious plasma containing 3 x 109 IU/mL and from ZeptoMetrix nucleic acid test standard (NATtrol™) for HBV containing 4 x 109 IU/mL using the PureLink Viral RNA/DNA Kit. Recovery of HBV NATtrol™ was comparable to infectious virus in patient plasma. qPCR was performed using the Platinum SYBR Green qPCR SuperMix-UDG w/ROX. NATtrol™ spiked samples (grey bars), HBV infected plasma (blue bars). |
Data provided by Carleen Pope, M.S. Senior Research Scientist, ZeptoMetrix Corporation. Visit www.zeptometrix.com for more information on NATtrol™ products.