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HeavyPeptide AQUA Custom Synthesis Service
Thermo Scientific HeavyPeptide AQUA Custom Synthesis Service provides isotopically labeled, AQUA-grade peptides for the relative and absolute quantitation of proteins at very low concentrations in complex protein mixtures. Peptides are synthesized using the latest Fmoc solid-phase technology and purified by HPLC. All HeavyPeptide sequences come with mass spectrometric analysis and analytical HPLC data. Additionally, we offer a wide range of labels, modifications and, scales.
HeavyPeptide AQUA Peptide specifications
| Peptide length | Up to 15* amino acids |
| Purity options | 95–97% |
| Formulation | Lyophilized or acetonitrile/H2O |
| Production time | 5–7 weeks standard or 2–3 weeks express** |
| Modifications | Variety of modifications and labels, including heavy amino acids (isotope-labeled) available |
| Delivery format | Glass vial |
*For longer peptide lengths, please download the quote form with the red button above and email your completed form to the email address in the white button above.
**AQUA Express requests are available for up to 10 sequences on a single order and must be approved for express turnaround time.
Available HeavyPeptide AQUA Peptide grades
| HeavyPeptide AQUA Basic | HeavyPeptide AQUA QuantPro | HeavyPeptide AQUA Ultimate | |
|---|---|---|---|
| Research stage | Discovery, confirmation, or validation, relative quantitation | Confirmation or validation, ideal for biomarker verification | Validation or clinical, ideal for absolute quantitation |
| Standard (Light) versions available | Yes | Yes | Yes |
| Formulation | Lyophilized | 5 pmol/µL in 5% (v/v) acetonitrile/H2O | 5 pmol/µL in 5% (v/v) acetonitrile/H2O |
| Concentration precision | NA | ±10–25% | ±5–10% |
| Peptide purity | >95% | >97% | >97% |
| Isotopic enrichment | >99% | >99% | >99% |
| Peptide length | Up to 15 amino acids† | Up to 15 amino acids† | Up to 15 amino acids† |
| Amount/aliquot number | 15–30 nmol / 1 aliquot | 10 nmol / 10 aliquots 40 nmol / 40 aliquots 96 nmol / 96 aliquots | 10 nmol / 10 aliquots 40 nmol / 40 aliquots 96 nmol / 96 aliquots |
| Quality control | Mass spectrometry Analytical HPLC Quantitative AAA | Mass spectrometry Analytical HPLC Quantitative AAA | Mass spectrometry Analytical HPLC Quantitative AAA |
| Shipment | Dry at room temperature | In solution on wet ice | In solution on wet ice |
| Delivery time | 5–7 weeks standard AQUA 2–3 weeks AQUA express†† | 5–7 weeks standard AQUA 2–3 weeks AQUA express†† | 5–7 weeks standard AQUA 2–3 weeks AQUA express†† |
† For longer peptide lengths, please download the quote form above and email the completed form to us.
††AQUA Express requests are available for up to 10 sequences on a single order and must be approved for express turnaround time.
Product options
- Additional light amino acids to extend the peptide length
- Additional heavy amino acid on each peptide
- Multiple solvents, concentrations, and aliquot sizes available
HeavyPeptide AQUA Peptide optional heavy amino acids
| Amino acid† | Code | Mass difference | Isotope | Isotopic enrichment |
|---|---|---|---|---|
| Alanine | A | +4 Da | U-13C3, 15N | >99% |
| Arginine | R | +10 Da | U-13C6, 15N4 | >99% |
| Isoleucine | I | +7 Da | U-13C6, 15N | >99% |
| Leucine | L | +7 Da | U-13C6, 15N | >99% |
| Lysine | K | +8 Da | U-13C6, 15N2 | >99% |
| Phenylalanine | F | +10 Da | U-13C9, 15N | >99% |
| Proline | P | +6 Da | U-13C5, 15N | >99% |
| Valine | V | +6 Da | U-13C5, 15N | >99% |
† Other amino acids on request.
Peptide modifications
- Single or double phosphorylation (pY, pT, or pS)
- Cysteine carbamidomethylation (CAM)‡
- Chloro-L-tyrosine
- Pyroglutamic acid
- Methionine oxidation (Met[O])
- Other modifications available on request
‡ CAM tends to cause cyclisation at the N-terminus. Fully cyclized form can be provided upon request.
Documents
Frequently asked questions
Q. What is the shelf life of a HeavyPeptide AQUA?
A. The shelf life is >12 months at –20°C. For long term storage we recommend –80°C. A HeavyPeptide AQUA cannot be frozen again after first utilization without risking a dramatic change in the standard concentration. Usually an aliquot of HeavyPeptide AQUA standard can be kept a few days at 4°C without significant changes. The stability and accuracy of a HeavyPeptide AQUA may vary with the sequence.
Q. Do HeavyPeptides AQUA in solution degrade faster vs. lyophilized ones?
Lyophilized peptide are less prone to degradation, oxidation, reduction, and other chemical modifications. However, it is usually not possible to reconstitute lyophilized peptides in a quantitative way as they tend to form colloids during the lyophilization process. This can result in unknown concentration of solubilized peptide, but one that can be measured using MS. In about 5% of the sequences, less than 10% of the peptide is fully solubilized. This problem cannot be detected by the operator. For products provided fully solubilized (QuantPro & Ultimate): if a chemical modification happens, it can be detected and the product discarded.
Q. How do you ship HeavyPeptides AQUA?
The Basic grade is shipped lyophilized protected with argon in a moisture proof sealed bag. Each bag includes molecular sieve to maintain the relative humidity close to ~0%. The Basic grade is shipped at room temperature. The QuantPro and Ultimate grades are provided in solution and shipped on wet ice.
Q. What information is needed to obtain an accurate quote?
A. All the information required to get an accurate quote is included in the Quote Request form.
HeavyPeptide AQUA Peptide specifications
| Peptide length | Up to 15* amino acids |
| Purity options | 95–97% |
| Formulation | Lyophilized or acetonitrile/H2O |
| Production time | 5–7 weeks standard or 2–3 weeks express** |
| Modifications | Variety of modifications and labels, including heavy amino acids (isotope-labeled) available |
| Delivery format | Glass vial |
*For longer peptide lengths, please download the quote form with the red button above and email your completed form to the email address in the white button above.
**AQUA Express requests are available for up to 10 sequences on a single order and must be approved for express turnaround time.
Available HeavyPeptide AQUA Peptide grades
| HeavyPeptide AQUA Basic | HeavyPeptide AQUA QuantPro | HeavyPeptide AQUA Ultimate | |
|---|---|---|---|
| Research stage | Discovery, confirmation, or validation, relative quantitation | Confirmation or validation, ideal for biomarker verification | Validation or clinical, ideal for absolute quantitation |
| Standard (Light) versions available | Yes | Yes | Yes |
| Formulation | Lyophilized | 5 pmol/µL in 5% (v/v) acetonitrile/H2O | 5 pmol/µL in 5% (v/v) acetonitrile/H2O |
| Concentration precision | NA | ±10–25% | ±5–10% |
| Peptide purity | >95% | >97% | >97% |
| Isotopic enrichment | >99% | >99% | >99% |
| Peptide length | Up to 15 amino acids† | Up to 15 amino acids† | Up to 15 amino acids† |
| Amount/aliquot number | 15–30 nmol / 1 aliquot | 10 nmol / 10 aliquots 40 nmol / 40 aliquots 96 nmol / 96 aliquots | 10 nmol / 10 aliquots 40 nmol / 40 aliquots 96 nmol / 96 aliquots |
| Quality control | Mass spectrometry Analytical HPLC Quantitative AAA | Mass spectrometry Analytical HPLC Quantitative AAA | Mass spectrometry Analytical HPLC Quantitative AAA |
| Shipment | Dry at room temperature | In solution on wet ice | In solution on wet ice |
| Delivery time | 5–7 weeks standard AQUA 2–3 weeks AQUA express†† | 5–7 weeks standard AQUA 2–3 weeks AQUA express†† | 5–7 weeks standard AQUA 2–3 weeks AQUA express†† |
† For longer peptide lengths, please download the quote form above and email the completed form to us.
††AQUA Express requests are available for up to 10 sequences on a single order and must be approved for express turnaround time.
Product options
- Additional light amino acids to extend the peptide length
- Additional heavy amino acid on each peptide
- Multiple solvents, concentrations, and aliquot sizes available
HeavyPeptide AQUA Peptide optional heavy amino acids
| Amino acid† | Code | Mass difference | Isotope | Isotopic enrichment |
|---|---|---|---|---|
| Alanine | A | +4 Da | U-13C3, 15N | >99% |
| Arginine | R | +10 Da | U-13C6, 15N4 | >99% |
| Isoleucine | I | +7 Da | U-13C6, 15N | >99% |
| Leucine | L | +7 Da | U-13C6, 15N | >99% |
| Lysine | K | +8 Da | U-13C6, 15N2 | >99% |
| Phenylalanine | F | +10 Da | U-13C9, 15N | >99% |
| Proline | P | +6 Da | U-13C5, 15N | >99% |
| Valine | V | +6 Da | U-13C5, 15N | >99% |
† Other amino acids on request.
Peptide modifications
- Single or double phosphorylation (pY, pT, or pS)
- Cysteine carbamidomethylation (CAM)‡
- Chloro-L-tyrosine
- Pyroglutamic acid
- Methionine oxidation (Met[O])
- Other modifications available on request
‡ CAM tends to cause cyclisation at the N-terminus. Fully cyclized form can be provided upon request.
Documents
Frequently asked questions
Q. What is the shelf life of a HeavyPeptide AQUA?
A. The shelf life is >12 months at –20°C. For long term storage we recommend –80°C. A HeavyPeptide AQUA cannot be frozen again after first utilization without risking a dramatic change in the standard concentration. Usually an aliquot of HeavyPeptide AQUA standard can be kept a few days at 4°C without significant changes. The stability and accuracy of a HeavyPeptide AQUA may vary with the sequence.
Q. Do HeavyPeptides AQUA in solution degrade faster vs. lyophilized ones?
Lyophilized peptide are less prone to degradation, oxidation, reduction, and other chemical modifications. However, it is usually not possible to reconstitute lyophilized peptides in a quantitative way as they tend to form colloids during the lyophilization process. This can result in unknown concentration of solubilized peptide, but one that can be measured using MS. In about 5% of the sequences, less than 10% of the peptide is fully solubilized. This problem cannot be detected by the operator. For products provided fully solubilized (QuantPro & Ultimate): if a chemical modification happens, it can be detected and the product discarded.
Q. How do you ship HeavyPeptides AQUA?
The Basic grade is shipped lyophilized protected with argon in a moisture proof sealed bag. Each bag includes molecular sieve to maintain the relative humidity close to ~0%. The Basic grade is shipped at room temperature. The QuantPro and Ultimate grades are provided in solution and shipped on wet ice.
Q. What information is needed to obtain an accurate quote?
A. All the information required to get an accurate quote is included in the Quote Request form.
Resources
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