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Pierce BSA Digest Standard | Pierce 6-Protein Mixture Standard | Pierce HeLa Protein Digest Standard | Pierce TMT11plex Yeast Digest Standard | |
Components (abbreviated) | BSA tryptic peptides | Lysozyme, BSA, Cytochrome C, Alcohol dehyrogenase, B-galactosidase, Apotransferrin (multi-species) | HeLa lysate peptides | Yeast lysate peptide digest |
Format | Lyophilized | Lyophilized | Lyophilized | Lyophilized |
Primary application | Protein sample control | Chromatography assessment | Complex sample control | Complex sample for TMT-labeling |
Complexity of standard | ++ | +++ | ++++ | +++ |
Recommended storage | -20°C | -20°C | -20°C | -20°C |
Order | Order | Order | Order |
Pierce Reserpine Standard for LC-MS | |
Components (abbreviated) | Reserpine molecule |
Format | Liquid |
Primary application | Sensitivity assessment |
Complexity of standard | + |
Recommended storage | 4°C |
Order |
Pierce Peptide Retention Time Calibration Mixture | Pierce LC-MS/MS System Suitability Standard (7 x 5 Mix) | |
Components (abbreviated) | 15 heavy tryptic peptides (yeast) | 7 yeast tryptic peptides (5 variations of light or heavy amino acids) |
Format | Frozen liquid | Frozen liquid |
Primary application | Chromatography assessment | Targeted MS Assay control |
Complexity of standard | ++ | ++ |
Recommended storage | -80°C | -80°C |
Order | Order |
Pierce Intact Protein Standard Mix | |
Components (abbreviated) | IGF-I LR3, Thioredoxin, Protein G, Carbonic Anhydrase II, Protein AG (chimeric), Exo Klenow |
Format | Lyophilized |
Primary application | Top down method development |
Complexity of standard | +++ |
Recommended storage | -20°C |
Order |
Pierce BSA Digest Standard | Pierce 6-Protein Mixture Standard | Pierce HeLa Protein Digest Standard | Pierce TMT11plex Yeast Digest Standard | |
Components (abbreviated) | BSA tryptic peptides | Lysozyme, BSA, Cytochrome C, Alcohol dehyrogenase, B-galactosidase, Apotransferrin (multi-species) | HeLa lysate peptides | Yeast lysate peptide digest |
Format | Lyophilized | Lyophilized | Lyophilized | Lyophilized |
Primary application | Protein sample control | Chromatography assessment | Complex sample control | Complex sample for TMT-labeling |
Complexity of standard | ++ | +++ | ++++ | +++ |
Recommended storage | -20°C | -20°C | -20°C | -20°C |
Order | Order | Order | Order |
Pierce Reserpine Standard for LC-MS | |
Components (abbreviated) | Reserpine molecule |
Format | Liquid |
Primary application | Sensitivity assessment |
Complexity of standard | + |
Recommended storage | 4°C |
Order |
Pierce Peptide Retention Time Calibration Mixture | Pierce LC-MS/MS System Suitability Standard (7 x 5 Mix) | |
Components (abbreviated) | 15 heavy tryptic peptides (yeast) | 7 yeast tryptic peptides (5 variations of light or heavy amino acids) |
Format | Frozen liquid | Frozen liquid |
Primary application | Chromatography assessment | Targeted MS Assay control |
Complexity of standard | ++ | ++ |
Recommended storage | -80°C | -80°C |
Order | Order |
Pierce Intact Protein Standard Mix | |
Components (abbreviated) | IGF-I LR3, Thioredoxin, Protein G, Carbonic Anhydrase II, Protein AG (chimeric), Exo Klenow |
Format | Lyophilized |
Primary application | Top down method development |
Complexity of standard | +++ |
Recommended storage | -20°C |
Order |
Pierce 利血平LC-MS标准品 | Pierce 肽保留时间校准混合物 | Pierce BSA 消化标准品 | Pierce 6种蛋白混合标准品 | Pierce完整蛋白标准品混合物 | Pierce HeLa 蛋白消化标准品 | |
组份 (缩写) | 利血平分子 | 15个重胰蛋白酶肽(酵母) | BSA胰蛋白酶肽 | 溶菌酶、BSA、细胞色素C、醇脱氢酶、B—半乳糖苷酶、脱铁运铁蛋白(多物种来源) | IGF-I LR3、硫氧还蛋白、G蛋白、碳酸酐酶II、AG蛋白(嵌合)、ExoKlenow | HeLa裂解肽 |
规格 | 液体 | 冷冻液体 | 冻干粉 | 冻干粉 | 冻干粉 | 冻干粉 |
主要应用 | 灵敏度评估 | 色谱评估 | 蛋白样品对照 | 色谱评估 | 自顶向下方法开发(Top down) | 复杂样品对照 |
标准品复杂度 | + | ++ | ++ | +++ | +++ | ++++ |
建议保存条件 | 4°C | -80°C | -20°C | -20°C | -20°C | -20°C |
订购 | 订购 | 订购 | 订购 | 订购 | 订购 |
Thermo Scientific Pierce消化指示剂是一种单一的非哺乳动物重组蛋白(26kDa),含有5个特征肽,可用于测定多个样品的消化效率和可重复性。蛋白序列和推荐用于监测样品的多肽详见产品说明书中。
在Thermo Scientific™ Vantage™三重四极杆质谱仪上500fg利血平的选择反应监测(SRM)示例。 在Hypersil GOLD aQ 2.1x20 mm Javelin色谱柱样品环上进行3次5μL,100 fg /μL Pierce™利血平进样的等量色谱图(70%甲醇300μL/ min 流速等度洗脱):30%水,0.05%甲酸(上图)。监测的SRM跃迁分别为609.3-195.1和609.3-448.2 m/z,峰宽0.2 FWHM Q1(下图)。
Thermo Scientific Pierce HeLa 消化标准品基峰色谱图。200 ng Pierce HeLa 蛋白消化标准品色谱图。 使用Thermo Scientific™ Acclaim™ PepMap™ 100 3μmx 75μmx 15 cm色谱柱(产品号:160321),以2-35%梯度(A:溶于水中的0.1% FA ,B:溶于100%乙腈中的0.1%FA),在300nL /分钟条件下,处理120分钟,并在Thermo Scientific™ LTQ Orbitrap™ XL质谱仪上进行检测。
在Thermo Scientific™ Vantage™三重四极杆质谱仪上500fg利血平的选择反应监测(SRM)示例。 在Hypersil GOLD aQ 2.1x20 mm Javelin色谱柱样品环上进行3次5μL,100 fg /μL Pierce™利血平进样的等量色谱图(70%甲醇300μL/ min 流速等度洗脱):30%水,0.05%甲酸(上图)。监测的SRM跃迁分别为609.3-195.1和609.3-448.2 m/z,峰宽0.2 FWHM Q1(下图)。
Thermo Scientific Pierce HeLa 消化标准品基峰色谱图。200 ng Pierce HeLa 蛋白消化标准品色谱图。 使用Thermo Scientific™ Acclaim™ PepMap™ 100 3μmx 75μmx 15 cm色谱柱(产品号:160321),以2-35%梯度(A:溶于水中的0.1% FA ,B:溶于100%乙腈中的0.1%FA),在300nL /分钟条件下,处理120分钟,并在Thermo Scientific™ LTQ Orbitrap™ XL质谱仪上进行检测。
仅供科研使用,不可用于诊断目的。