Thermo Scientific Pierce标准品专为广泛应用而设计,包括灵敏度评估、消化效率测定、色谱评估、和/或作为样品分析对照。产品范围涵盖了从单组分制剂到复杂的基于人体的消化物,且都附有质量分析证书。
  • 更多选择——各种不同的标准品,能够满足广泛的应用/需求
  • 稳定——在建议温度下,可保存超过一年的时间
  • 确保验证——所有标准品均由ISO 9001设备制造,并通过Thermo Scientific质谱仪检测和全面测试

为您的应用选择合适标准品

 
 Pierce BSA Digest StandardPierce 6-Protein Mixture StandardPierce HeLa Protein Digest StandardPierce TMT11plex Yeast Digest Standard
Components
(abbreviated)
BSA tryptic peptidesLysozyme, BSA, Cytochrome C, Alcohol dehyrogenase, B-galactosidase, Apotransferrin (multi-species)HeLa lysate peptidesYeast lysate peptide digest
FormatLyophilizedLyophilizedLyophilizedLyophilized
Primary applicationProtein sample controlChromatography assessmentComplex sample controlComplex sample for TMT-labeling
Complexity of standard++++++++++++
Recommended storage-20°C-20°C-20°C-20°C
 Order Order OrderOrder
 
 Pierce Reserpine Standard for LC-MS
Components
(abbreviated)
Reserpine molecule
FormatLiquid
Primary applicationSensitivity assessment
Complexity of standard+
Recommended storage4°C
 Order
 
 Pierce Peptide Retention Time Calibration MixturePierce LC-MS/MS System Suitability Standard (7 x 5 Mix)
Components
(abbreviated)
15 heavy tryptic peptides (yeast)7 yeast tryptic peptides (5 variations of light or heavy amino acids)
FormatFrozen liquidFrozen liquid
Primary applicationChromatography assessmentTargeted MS Assay control
Complexity of standard

++

++
Recommended storage-80°C-80°C
 Order Order
 
 Pierce Intact Protein Standard Mix
Components
(abbreviated)
IGF-I LR3, Thioredoxin, Protein G, Carbonic Anhydrase II, Protein AG (chimeric), Exo Klenow
FormatLyophilized
Primary applicationTop down method development
Complexity of standard+++
Recommended storage-20°C
 Order
 
 Pierce BSA Digest StandardPierce 6-Protein Mixture StandardPierce HeLa Protein Digest StandardPierce TMT11plex Yeast Digest Standard
Components
(abbreviated)
BSA tryptic peptidesLysozyme, BSA, Cytochrome C, Alcohol dehyrogenase, B-galactosidase, Apotransferrin (multi-species)HeLa lysate peptidesYeast lysate peptide digest
FormatLyophilizedLyophilizedLyophilizedLyophilized
Primary applicationProtein sample controlChromatography assessmentComplex sample controlComplex sample for TMT-labeling
Complexity of standard++++++++++++
Recommended storage-20°C-20°C-20°C-20°C
 Order Order OrderOrder
 
 Pierce Reserpine Standard for LC-MS
Components
(abbreviated)
Reserpine molecule
FormatLiquid
Primary applicationSensitivity assessment
Complexity of standard+
Recommended storage4°C
 Order
 
 Pierce Peptide Retention Time Calibration MixturePierce LC-MS/MS System Suitability Standard (7 x 5 Mix)
Components
(abbreviated)
15 heavy tryptic peptides (yeast)7 yeast tryptic peptides (5 variations of light or heavy amino acids)
FormatFrozen liquidFrozen liquid
Primary applicationChromatography assessmentTargeted MS Assay control
Complexity of standard

++

++
Recommended storage-80°C-80°C
 Order Order
 
 Pierce Intact Protein Standard Mix
Components
(abbreviated)
IGF-I LR3, Thioredoxin, Protein G, Carbonic Anhydrase II, Protein AG (chimeric), Exo Klenow
FormatLyophilized
Primary applicationTop down method development
Complexity of standard+++
Recommended storage-20°C
 Order
 
 Pierce 利血平LC-MS标准品Pierce 肽保留时间校准混合物Pierce BSA 消化标准品Pierce 6种蛋白混合标准品Pierce完整蛋白标准品混合物Pierce HeLa 蛋白消化标准品
组份
(缩写)
利血平分子15个重胰蛋白酶肽(酵母)BSA胰蛋白酶肽溶菌酶、BSA、细胞色素C、醇脱氢酶、B—半乳糖苷酶、脱铁运铁蛋白(多物种来源)IGF-I LR3、硫氧还蛋白、G蛋白、碳酸酐酶II、AG蛋白(嵌合)、ExoKlenowHeLa裂解肽
规格液体冷冻液体冻干粉冻干粉冻干粉冻干粉
主要应用灵敏度评估色谱评估蛋白样品对照色谱评估自顶向下方法开发(Top down)复杂样品对照
标准品复杂度+

++

++++++++++++
建议保存条件4°C-80°C-20°C-20°C-20°C-20°C
 订购订购订购订购订购 订购

需要检测消化效率的标准品?

Thermo Scientific Pierce消化指示剂是一种单一的非哺乳动物重组蛋白(26kDa),含有5个特征肽,可用于测定多个样品的消化效率和可重复性。蛋白序列和推荐用于监测样品的多肽详见产品说明书中。

性能数据

Example selected reaction monitoring (SRM) of 500fg reserpine on a Thermo Scientific™ Vantage™ triple quadrupole mass spectrometer.

在Thermo Scientific™ Vantage™三重四极杆质谱仪上500fg利血平的选择反应监测(SRM)示例。 在Hypersil GOLD aQ 2.1x20 mm Javelin色谱柱样品环上进行3次5μL,100 fg /μL Pierce™利血平进样的等量色谱图(70%甲醇300μL/ min 流速等度洗脱):30%水,0.05%甲酸(上图)。监测的SRM跃迁分别为609.3-195.1和609.3-448.2 m/z,峰宽0.2 FWHM Q1(下图)。

Thermo Scientific Pierce HeLa Digest Standard base peak chromatogram.

Thermo Scientific Pierce HeLa 消化标准品基峰色谱图。200 ng Pierce HeLa 蛋白消化标准品色谱图。 使用Thermo Scientific™ Acclaim™ PepMap™ 100 3μmx 75μmx 15 cm色谱柱(产品号:160321),以2-35%梯度(A:溶于水中的0.1% FA ,B:溶于100%乙腈中的0.1%FA),在300nL /分钟条件下,处理120分钟,并在Thermo Scientific™ LTQ Orbitrap™ XL质谱仪上进行检测。

Example selected reaction monitoring (SRM) of 500fg reserpine on a Thermo Scientific™ Vantage™ triple quadrupole mass spectrometer.

在Thermo Scientific™ Vantage™三重四极杆质谱仪上500fg利血平的选择反应监测(SRM)示例。 在Hypersil GOLD aQ 2.1x20 mm Javelin色谱柱样品环上进行3次5μL,100 fg /μL Pierce™利血平进样的等量色谱图(70%甲醇300μL/ min 流速等度洗脱):30%水,0.05%甲酸(上图)。监测的SRM跃迁分别为609.3-195.1和609.3-448.2 m/z,峰宽0.2 FWHM Q1(下图)。

Thermo Scientific Pierce HeLa Digest Standard base peak chromatogram.

Thermo Scientific Pierce HeLa 消化标准品基峰色谱图。200 ng Pierce HeLa 蛋白消化标准品色谱图。 使用Thermo Scientific™ Acclaim™ PepMap™ 100 3μmx 75μmx 15 cm色谱柱(产品号:160321),以2-35%梯度(A:溶于水中的0.1% FA ,B:溶于100%乙腈中的0.1%FA),在300nL /分钟条件下,处理120分钟,并在Thermo Scientific™ LTQ Orbitrap™ XL质谱仪上进行检测。

仅供科研使用,不可用于诊断目的。