Organelle Isolation Using Magnetic Beads | Thermo Fisher Scientific

Dynabeads® Magnetic Beads can be used to isolate a number of different organelles and organelle-specific proteins. This magnetic separation technology enables the specific isolation of true fractions in small volumes, and can replace time-consuming and labor-intensive density gradient centrifugations.

Advantages include:

Rapid, high-purity isolations from crude fractions or fractions of common densities
Morphologically intact target organelles can be fixed for EM studies or solubilized directly on the bead surface
Immunomagnetically isolated organelles can be assayed by conventional techniques
Easy protocol yields high-purity functional organelles

As more and more organelle-specific markers are identified, the corresponding immunomagnetic isolation of subgroups of organelles can be performed. Examples of organelles or organelle fractions that have been isolated with Dynabeads® include exosomes, mitochondria, peroxisomes, kidney glomeruli, and endocytic and secretory compartments.

View selected Dynabeads® references

Compare Dynabeads® organelle isolation methods

1. Select your application	Application specification	2. Click on the Solution	Advantage
Isolation of fragile organelles or organelle fractions in general	Using a mouse primary antibody	Dynabeads® M-280 Sheep anti-Mouse IgG	Direct or indirect isolation of organelles made more efficient with two layers of Abs.
	Using a rabbit primary antibody	Dynabeads® M-280 Sheep anti-Rabbit IgG	Direct or indirect isolation of organelles made more efficient with two layers of Abs.
	Antibodies made in any other species	Dynabeads® Protein G Dynabeads® Protein A	Efficient direct or indirect isolation of organelles.
Isolation of large organelle fractions or isolation from a viscous sample	Using antibodies made in a specific species	Dynabeads® M-450 with a secondary antibody against the corresponding primary Ab species	More efficient pull to the magnet in viscous samples
Isolation of organelles using a ligand that is not an antibody	When high yield is important	Dynabeads® M-280 Tosylactivated	Easy coupling of a molecule onto these hydrophobic beads for efficient organelle isolation
Isolation of organelles using a ligand that is not an antibody	When high purity is important	Dynabeads® M-270 Epoxy	Coupling of a molecule onto these hydrophilic beads for very high purity organelle isolation

Note: 4.5 µm Dynabeads® Magnetic Beads can be used instead

仅供科研使用，不可用于诊断目的。