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Fast, accurate KRAS variant detection... Because you can't afford to get it wrong.
Sequencing is increasingly used in the diagnosis and monitoring of diseases including cancer. With gold-standard accuracy and the ability to detect new genetic variants, Sanger sequencing enables precision medicine.
Role of Sanger sequencing in KRAS variant identification
Kirsten rat sarcoma viral oncogene homologue (KRAS) is a hyper-mutated oncogene across all cancer types. Tumors with KRAS mutations are resistant to many chemotherapeutics including those targeting the epidermal growth factor receptor (EGFR). KRAS mutations can also develop during treatment. Additionally, treatments targeting specific KRAS mutations are currently on the market with more under development.
Ninety-eight percent (98%) of KRAS mutations occur within codons 12 or 13 (exon 2) and codon 61 (exon 3). A large variety of mutations within these codons exist and their frequency varies by cancer type. Sanger sequencing offers several key attributes useful for the detection of KRAS mutations.
Benefits of Sanger sequencing for KRAS variant detection
Sensitive and accurate
Simple workflow
Unambiguous results
Rapid time to results
KRAS variant detection workflow
Figure 1. Simplified, accurate workflow to detect KRAS mutations. Using reagents such as the BigDye Direct kit saves steps. The protocol is flexible and can be used with a variety of instruments.
Accurate detection of KRAS mutations using Sanger sequencing
Thermo Fisher Scientific has developed a complete and streamlined protocol for the detection of unknown and common KRAS variants including G12C, G12D, and G13D.
Featured products and resources
KRAS variant detection assays
We identified primers for the detection of unknown and common KRAS variants including G12C, G12D, and G13D.
How to order
Order the same assays referenced in the KRAS variant identification protocol guide by following these steps:
Step 1: Use our Primer Design Tool to search for validated primers using these Assay IDs:
- Hs00679698_CE
- Hs00532900_CE
Step 2: Add the primers with the below selections:
- Primer 5’tail Option: M13-tailed
- Purification: HPLC
- Primer Pair / FWD /REV: Pair
SeqStudio Flex: a genetic analyzer suited for detection of genetic variants
The SeqStudio Flex Series genetic analyzers are our most advanced mid-throughput genetic analyzers with the flexibility, usability, connectivity, and serviceability to keep your laboratory up and running at maximum efficiency. Get critical results faster with urgent sample reprioritization capabilities and complete scheduling flexibility.
Obtain sequence reads up to 40% faster using BigDye Direct Sanger Sequencing Kit
The BigDye Direct Sanger Sequencing Kit helps enable accurate data. All steps from PCR to capillary electrophoresis are performed in a single tube, reducing hands-on time and the possibility of introducing experimental variability.
Style Sheet for Global Design System
仅供科研使用，不可用于诊断目的。