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The green fluorescent protein (GFP) from the jellyfish Aequorea victoria is a versatile marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression.1-5 We offer the anti-GFP antibody as a rabbit polyclonal, monoclonal, or IgG fraction, two mouse monoclonal antibodies, and a chicken IgY fraction. All six anti-GFP antibodies are suited for detection of native GFP, GFP variants, and most GFP fusion proteins by western blot analysis while the rabbit and mouse antibodies are also useful for immunoprecipitation. A summary of the validated applications for all of these antibodies can be found in Table 1.
The anti-GFP rabbit polyclonal antibody is raised against GFP isolated directly from Aequorea victoria and is available as a complete antiserum (A6455) or as an IgG fraction purified by ion-exchange chromatography (A11122).
Like our multi-purpose polyclonal anti-GFP antibody, the rabbit anti-GFP monoclonal antibody (G10362) is raised against full-length GFP; it is suitable for immunoprecipitation, immunohistochemistry, and western blotting.
Anti-GFP mouse monoclonal antibody 3E6 (A11120) is useful for immunoprecipitation, immunocytochemical localization, and immunosorbent assays (ELISA).
Anti-GFP mouse monoclonal antibody 11E5 (A11121) is optimized for western analysis, allowing colorimetric detection of as little as 10 ng of GFP or GFP-fusion proteins, or chemiluminescent detection of picogram quantities.
The chicken anti-GFP antibody (A10262) is raised against GFP isolated directly from Aequorea victoria (Table 1), and the IgY fraction is purified by affinity purification. The chicken IgY lacks a classic “Fc” domain and does not bind to mammalian IgG Fc receptors, resulting in lower backgrounds during immunostaining protocols. The chicken IgY is also antigenically different from the mammalian IgG, allowing you to perform double immunostaining experiments using antibodies from multiple species.
Table 1. Anti-GFP Antibodies and Their Applications.
Catalog No. | Host & Type | Amount | Application# | Form |
---|---|---|---|---|
A6455 | Rabbit Polyclonal | 100 µL | IP, IHC, WB | Serum |
A10262 | Chicken Polyclonal | 100 µL* | ICC, WB | IgY Fraction |
A11122 | Rabbit Polyclonal | 100 µL* | IP, IHC, WB | IgG Fraction |
A11120 | Mouse Monoclonal | 100 µg | IP, IHC | IgG2a |
A11121 | Mouse Monoclonal | 100 µg | WB | IgG1 |
G10362 | Rabbit Monoclonal | 100 µg | ELISA, IP, IHC, WB, Flow | IgG |
* 2 mg/mL. # Immunoprecipitation (IP), immunohistochemistry (IHC), western blot (WB) detection, immunocytochemistry (ICC), and flow cytometry (Flow).