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实时PCR基础知识支持
您可通过我们的“入门”和“故障排除”部分内容了解最常见问题的解决方案。而通过我们的“指南和工具”部分，则可接触到我们全面的产品相关支持资源组合。
Getting Started
- How long can I store the cDNA from my reverse transcription step?
- Do I need to treat my sample with DNAse? If so, how should I do it?
- Do I have to purify RNA before the qPCR reaction?
- How much first-strand cDNA should be used in a PCR reaction?
- What is the difference between absolute quantification (AQ) and relative quantification (RQ)? How do I choose which method to use?
Troubleshooting
- What should I do if I have extra peaks in my melt curve? What does this mean?
- My amplification curves have a funny shape. What is causing this?
- What can I do to improve the sensitivity of my assay?
- How do I do a no-RT control reaction with the SuperScript® VILO™ Master Mix?
- How do I measure how much cDNA I have after the reverse transcription?
qPCR指南和工具
- 概述: qPCR 培训
- 概述: qPCR (Thermo Scientific™)
- 概述: Basic Principles of RT-qPCR (Thermo Scientific™)
- 概述: TaqMan实时荧光定量PCR分析试剂盒
- 概述: TaqMan分析与SYBR Green化学法之间的比较
- 概述: Cells-to-Ct
- 概述: 您可以使用qPCR进行哪些应用？
- 概述: Plastic Consumables (Thermo Scientific™)
- 对比表: 一步法与两步法试剂盒之间的比较
- 对比表: 绝对定量与相对定量之间的比较
- General: qPCR Resource Library (Thermo Scientific™)
- 指南: 实时荧光定量PCR手册
- 产品: 实时荧光定量PCR (qPCR)
- 选择表: 用于基因表达的预混液
- 选择: Choosing your PCR Accessories (Tubes, Strips, Plates, etc.)
- 视频: Ask TaqMan 视频库
- 视频: qPCR 技术
- 视频: How to Control Overpipetting Errors When Performing qPCR (Thermo Scientific™)
- 视频: OEM Manufacturing Capabilities for Plastics Consumables (Thermo Scientific™)
- 视频: Flexible Consumables for PCR & qPCR (Thermo Scientific™)
- 工作流程: 使用qRT-PCR分析基因表达
- 培训: Real-Time Quantitative PCR Training Series-Module 1: Fundamentals of Real-Time PCR (54 min)
- 培训: Real-Time Quantitative PCR Training Series-Module 2: Real-Time PCR Assay Design (50 min)
- 培训: Real-Time Quantitative PCR Training Series-Module 3: Normalization (46 min)
- 培训: Real-Time Quantitative PCR Training Series-Module 4: Multiplexing (30 min)
- 培训: Real-Time Quantitative PCR Training Series-Module 5: Real-Time PCR Reagent Choices (40 min)
- 培训: Real-Time Quantitative PCR Training Series-Module 6: Theory of Real-Time Quantitative PCR Analysis (53 min)
- 在线研讨会: Fundamentals of Real-Time PCR
- 在线研讨会: How to Avoid Common Mistakes in the qPCR Workflow (Thermo Scientific™)
- 在线研讨会: The Essentials of Real-Time PCR (Thermo Scientific™)
- 软件: ExpressionSuite™ 软件 - 用于相对定量的数据分析
- 软件: DataAssist™ 软件 - 用于相对定量的数据分析
- 软件: Primer Express Software v3.0.1 - 用于TaqMan探针和SYBR引物设计
- 工具: TaqMan Assay Search Tool
- 工具: OligoPerfect™ - 用于SYBR引物设计的免费程序
- 工具: 基因表达故障排除工具
- 工具: Tm Calculator (Thermo Scientific™)
- 工具: Multiple Primer Analyzer (Thermo Scientific™) - helps you analyze and compare multiple primer sequences simultaneously
- 工具: Reaction Setup with DyNAmo qPCR Kits (Thermo Scientific™)
- 工具: qPCR Efficiency Calculator (Thermo Scientific™) - gives the amplification efficiency of a qPCR reaction based on the slope of the standard curve
- Reagent Rewards: 一款基于应用的程序，能够将试剂订购积分兑换为奖品
- Real-Time PCR（实时定量PCR）: 一款提供实时PCR相关资源的软件，包括视频，一个交互式疑难解答工具，和一个反应体系计算器
- Daily Calcs: 可通过此应用程序计算摩尔浓度、稀释度、分子量等
服务
处理您所有关于仪器和服务需求的一站式平台。
我们的遗传分析定制产品和服务团队能够为您提供广泛的选择，包括定制预混液等试剂，或定制TaqMan分析试剂盒。我们将与您合作开发一款定制的客户解决方案，来匹配您的应用需求。
当您尚未拥有全部必需的设备或试剂时，我们的定制产品和服务团队可帮助您开展大型项目。我们的合约式研究工作包括设计和执行实验，针对各种不同应用实施样品制备、数据分析和生成报告。
仅限研究用途，不可用于诊断操作。