Fluorophore Selection Guide | Thermo Fisher Scientific

Fluorophores enable researchers to expand the limits of their multicolor experiments in high content analysis, microscopy, and flow cytometry platforms. Our fluorescent dyes are offered conjugated to antibodies, streptavidin, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. They are also available as labeling reagents and kits allowing researchers to create labeled biomolecules for use in various applications as well as for probing biological structures, functions, and interactions. You can find information about each fluorophore by browsing the tables below.

On this page, you can also find fluorophore brands to explore our different dye brands and resources to explore protocols and selection guide posters for flow cytometry and imaging.

Explore fluorophores based on emission wavelength

Explore fluorophores based on excitation wavelength

Fluorophore	Excitation	Emission	Chemical property	Platform
Brilliant Ultra Violet 395	347	399	Polymer dye	flow cytometry
Alexa Fluor 350	343	441	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
Brilliant Ultra Violet 496	348	493	Tandem polymer dye	flow cytometry
Qdot 525 Probe	300	525	Quantum dot	microscopy; flow cytometry
Brilliant Ultra Violet 563	349	563	Tandem polymer dye	flow cytometry
Qdot 565 Probe	300	564	Quantum dot	microscopy; flow cytometry
Qdot 605 Probe	300	603	Quantum dot	microscopy; flow cytometry
Brilliant Ultra Violet 615	350	607	Tandem polymer dye	flow cytometry
Qdot 655 Probe	300	654	Quantum dot	microscopy; flow cytometry
Brilliant Ultra Violet 661	349	659	Tandem polymer dye	flow cytometry
Qdot 705 Probe	300	702	Quantum dot	microscopy; flow cytometry
Brilliant Ultra Violet 737	350	740	Tandem polymer dye	flow cytometry
NovaFluor UV 765	358	763	Phiton-based DNA scaffold dye	flow cytometry
Qdot 800 Probe	300	792	Quantum dot	microscopy; flow cytometry
Brilliant Ultra Violet 805	349	804	Tandem polymer dye	flow cytometry

Design tools that can help you get an excellent combination of dyes for your experiment

Fluorescence SpectraViewer—compare the spectra and spectral properties of multiple fluorophores
Panel Builder—choose the optimal combination of fluorophores for your flow cytometry experiments
Stain-iT Cell Staining Simulator—visualize staining your cell without wasting your reagents, antibodies, or time
EVOS Light Cube Selection Guide—choose the light cube that best matches your dye

Fluorophore	Excitation	Emission	Chemical property	Platform
Alexa Fluor 405	401	422	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
Brilliant Violet 421	406	423	Polymer dye	flow cytometry
Super Bright 436	413	431	Polymer dye	flow cytometry
eFluor 450	405	445	Small organic dye	flow cytometry
Pacific Blue	404	455	Small organic dye	flow cytometry
Coumarin and Coumarin Derivatives	387	470	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
Brilliant Violet 480	437	478	Polymer dye	flow cytometry
Cyan Fluorescent Protein (CFP)	435	485	Expressed fluorescent protein	microscopy; flow cytometry
Aluora 430	427	499	Small organic dye	microscopy
eFluor 506	419	508	Small organic dye	flow cytometry
Pacific Green	411	510	Small organic dye	flow cytometry
Pacific Orange	400	551	Small organic dye	flow cytometry
Super Bright 600	414	601	Tandem polymer dye	flow cytometry
Brilliant Violet 605	405	605	Tandem polymer dye	flow cytometry
Super Bright 645	414	645	Tandem polymer dye	flow cytometry
Brilliant Violet 650	407	646	Tandem polymer dye	flow cytometry
NovaFluor Violet 690	408	691	Phiton-based DNA scaffold dye	flow cytometry
Super Bright 702	413	702	Tandem polymer dye	flow cytometry
Brilliant Violet 711	406	711	Tandem polymer dye	flow cytometry
NovaFluor Violet 745	391	743	Phiton-based DNA scaffold dye	flow cytometry
Super Bright 780	413	780	Tandem polymer dye	flow cytometry
Brilliant Violet 786	406	788	Tandem polymer dye	flow cytometry
NovaFluor Violet 800	408	743	Phiton-based DNA scaffold dye	flow cytometry

Design tools that can help you get an excellent combination of dyes for your experiment

Fluorophore	Excitation	Emission	Chemical property	Platform
Green Fluorescent Protein (GFP)	488	510	Expressed fluorescent protein	microscopy; flow cytometry
BODIPY FL	502	511	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
NovaFluor Blue 510	493	513	Phiton-based DNA scaffold dye	flow cytometry
Fluorescein (FITC)	498	517	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
Aluora 488	493	518	Small organic dye	microscopy
Alexa Fluor 488	499	520	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
Oregon Green 488	498	526	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
NovaFluor Blue 530	508	527	Phiton-based DNA scaffold dye	flow cytometry
NovaFluor Blue 555	493	555	Phiton-based DNA scaffold dye	flow cytometry
NovaFluor Blue 585	493	586	Phiton-based DNA scaffold dye	flow cytometry
NovaFluor Blue 610-30S	508	615	Phiton-based DNA scaffold dye	flow cytometry
NovaFluor Blue 610-70S	492	616	Phiton-based DNA scaffold dye	flow cytometry
NovaFluor Blue 660-40S	508	664	Phiton-based DNA scaffold dye	flow cytometry
NovaFluor Blue 660-120S	492	665	Phiton-based DNA scaffold dye	flow cytometry
PerCP-Cyanine5.5	489	679	Protein-based tandem dye	flow cytometry
NovaFluor Blue 690	494	690	Phiton-based DNA scaffold dye	flow cytometry
PerCP-eFluor 710	482	708	Protein-based tandem dye	flow cytometry
NovaFluor Blue 725	492	725	Phiton-based DNA scaffold dye	flow cytometry
NovaFluor Blue 760	490	764	Phiton-based DNA scaffold dye	flow cytometry
NovaFluor Blue 800	493	806	Phiton-based DNA scaffold dye	flow cytometry

Design tools that can help you get an excellent combination of dyes for your experiment

Fluorophore	Excitation	Emission	Chemical property	Platform
Aluora 514	512	529	Small organic dye	microscopy
Alexa Fluor 532	534	553	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
Cy3	554	566	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
NovaFluor Yellow 570	551	566	Phiton-based DNA scaffold dye	flow cytometry
Aluora 555	553	567	Small organic dye	microscopy
Alexa Fluor 555	553	568	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
Alexa Fluor 561	556	569	Small organic dye	flow cytometry
Alexa Fluor 546	561	572	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
R-phycoerythrin (R-PE)	565	576	Protein-based fluorophore	flow cytometry
Tetramethylrhodamine (TRITC)	552	578	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
Red Fluorescent Protein (RFP)	555	584	Expressed fluorescent protein	microscopy; flow cytometry
NovaFluor Yellow 590	563	594	Phiton-based DNA scaffold dye	flow cytometry
Alexa Fluor 568	579	603	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
PE-eFluor 610	565	606	Protein-based tandem dye	flow cytometry
Texas Red (and Texas Red-X)	595	613	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
NovaFluor Yellow 610	551	614	Phiton-based DNA scaffold dye	flow cytometry
Aluora 594	589	615	Small organic dye	microscopy
Alexa Fluor 594	590	618	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
NovaFluor Yellow 660	550	664	Phiton-based DNA scaffold dye	flow cytometry
NovaFluor Yellow 690	551	691	Phiton-based DNA scaffold dye	flow cytometry
NovaFluor Yellow 700	551	701	Phiton-based DNA scaffold dye	flow cytometry
NovaFluor Yellow 730	551	730	Phiton-based DNA scaffold dye	flow cytometry
NovaFluor Yellow 755	552	755	Phiton-based DNA scaffold dye	flow cytometry
PE-Cyanine7	569	780	Protein-based tandem dye	flow cytometry
NovaFluor Yellow 810	551	813	Phiton-based DNA scaffold dye	flow cytometry

Design tools that can help you get an excellent combination of dyes for your experiment

Fluorophore	Excitation	Emission	Chemical property	Platform
NovaFluor Red 660	637	658	Phiton-based DNA scaffold dye	flow cytometry
Allophycocyanin (APC)	651	660	Protein-based fluorophore	flow cytometry
Cy5	647	665	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
eFluor 660	651	668	Small organic dye	flow cytometry
Aluora 647	652	670	Small organic dye	microscopy
Alexa Fluor 647	650	671	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
NovaFluor Red 685	637	685	Phiton-based DNA scaffold dye	flow cytometry
Alexa Fluor 660	663	691	Small organic dye	flow cytometry
NovaFluor Red 700	637	701	Phiton-based DNA scaffold dye	flow cytometry
Alexa Fluor 680	681	704	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
Aluora 700	687	706	Small organic dye	microscopy
NovaFluor Red 710	637	713	Phiton-based DNA scaffold dye	flow cytometry
Alexa Fluor 700	696	719	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
NovaFluor Red 725	636	725	Phiton-based DNA scaffold dye	flow cytometry
NovaFluor Red 755	636	755	Phiton-based DNA scaffold dye	flow cytometry

Design tools that can help you get an excellent combination of dyes for your experiment

Fluorophore	Excitation	Emission	Chemical property	Platform
Alexa Fluor 750	752	776	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
Aluora 750	757	783	Small organic dye	microscopy
APC-eFluor 780	756	785	Protein-based tandem dye	flow cytometry

Design tools that can help you get an excellent combination of dyes for your experiment

UV
(300-379 nm)

Fluorophore	Excitation	Emission	Chemical property	Platform
Brilliant Ultra Violet 395	347	399	Polymer dye	flow cytometry
Alexa Fluor 350	343	441	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
Brilliant Ultra Violet 496	348	493	Tandem polymer dye	flow cytometry
Qdot 525 Probe	300	525	Quantum dot	microscopy; flow cytometry
Brilliant Ultra Violet 563	349	563	Tandem polymer dye	flow cytometry
Qdot 565 Probe	300	564	Quantum dot	microscopy; flow cytometry
Qdot 605 Probe	300	603	Quantum dot	microscopy; flow cytometry
Brilliant Ultra Violet 615	350	607	Tandem polymer dye	flow cytometry
Qdot 655 Probe	300	654	Quantum dot	microscopy; flow cytometry
Brilliant Ultra Violet 661	349	659	Tandem polymer dye	flow cytometry
Qdot 705 Probe	300	702	Quantum dot	microscopy; flow cytometry
Brilliant Ultra Violet 737	350	740	Tandem polymer dye	flow cytometry
NovaFluor UV 765	358	763	Phiton-based DNA scaffold dye	flow cytometry
Qdot 800 Probe	300	792	Quantum dot	microscopy; flow cytometry
Brilliant Ultra Violet 805	349	804	Tandem polymer dye	flow cytometry

Design tools that can help you get an excellent combination of dyes for your experiment

Violet
(380-439 nm)

Fluorophore	Excitation	Emission	Chemical property	Platform
Alexa Fluor 405	401	422	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
Brilliant Violet 421	406	423	Polymer dye	flow cytometry
Super Bright 436	413	431	Polymer dye	flow cytometry
eFluor 450	405	445	Small organic dye	flow cytometry
Pacific Blue	404	455	Small organic dye	flow cytometry
Coumarin and Coumarin Derivatives	387	470	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
Brilliant Violet 480	437	478	Polymer dye	flow cytometry
Cyan Fluorescent Protein (CFP)	435	485	Expressed fluorescent protein	microscopy; flow cytometry
Aluora 430	427	499	Small organic dye	microscopy
eFluor 506	419	508	Small organic dye	flow cytometry
Pacific Green	411	510	Small organic dye	flow cytometry
Pacific Orange	400	551	Small organic dye	flow cytometry
Super Bright 600	414	601	Tandem polymer dye	flow cytometry
Brilliant Violet 605	405	605	Tandem polymer dye	flow cytometry
Super Bright 645	414	645	Tandem polymer dye	flow cytometry
Brilliant Violet 650	407	646	Tandem polymer dye	flow cytometry
NovaFluor Violet 690	408	691	Phiton-based DNA scaffold dye	flow cytometry
Super Bright 702	413	702	Tandem polymer dye	flow cytometry
Brilliant Violet 711	406	711	Tandem polymer dye	flow cytometry
NovaFluor Violet 745	391	743	Phiton-based DNA scaffold dye	flow cytometry
Super Bright 780	413	780	Tandem polymer dye	flow cytometry
Brilliant Violet 786	406	788	Tandem polymer dye	flow cytometry
NovaFluor Violet 800	408	743	Phiton-based DNA scaffold dye	flow cytometry

Design tools that can help you get an excellent combination of dyes for your experiment

Blue
(440-509 nm)

Fluorophore	Excitation	Emission	Chemical property	Platform
Green Fluorescent Protein (GFP)	488	510	Expressed fluorescent protein	microscopy; flow cytometry
BODIPY FL	502	511	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
NovaFluor Blue 510	493	513	Phiton-based DNA scaffold dye	flow cytometry
Fluorescein (FITC)	498	517	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
Aluora 488	493	518	Small organic dye	microscopy
Alexa Fluor 488	499	520	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
Oregon Green 488	498	526	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
NovaFluor Blue 530	508	527	Phiton-based DNA scaffold dye	flow cytometry
NovaFluor Blue 555	493	555	Phiton-based DNA scaffold dye	flow cytometry
NovaFluor Blue 585	493	586	Phiton-based DNA scaffold dye	flow cytometry
NovaFluor Blue 610-30S	508	615	Phiton-based DNA scaffold dye	flow cytometry
NovaFluor Blue 610-70S	492	616	Phiton-based DNA scaffold dye	flow cytometry
NovaFluor Blue 660-40S	508	664	Phiton-based DNA scaffold dye	flow cytometry
NovaFluor Blue 660-120S	492	665	Phiton-based DNA scaffold dye	flow cytometry
PerCP-Cyanine5.5	489	679	Protein-based tandem dye	flow cytometry
NovaFluor Blue 690	494	690	Phiton-based DNA scaffold dye	flow cytometry
PerCP-eFluor 710	482	708	Protein-based tandem dye	flow cytometry
NovaFluor Blue 725	492	725	Phiton-based DNA scaffold dye	flow cytometry
NovaFluor Blue 760	490	764	Phiton-based DNA scaffold dye	flow cytometry
NovaFluor Blue 800	493	806	Phiton-based DNA scaffold dye	flow cytometry

Design tools that can help you get an excellent combination of dyes for your experiment

Green/Yellow
(510-599 nm)

Fluorophore	Excitation	Emission	Chemical property	Platform
Aluora 514	512	529	Small organic dye	microscopy
Alexa Fluor 532	534	553	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
Cy3	554	566	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
NovaFluor Yellow 570	551	566	Phiton-based DNA scaffold dye	flow cytometry
Aluora 555	553	567	Small organic dye	microscopy
Alexa Fluor 555	553	568	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
Alexa Fluor 561	556	569	Small organic dye	flow cytometry
Alexa Fluor 546	561	572	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
R-phycoerythrin (R-PE)	565	576	Protein-based fluorophore	flow cytometry
Tetramethylrhodamine (TRITC)	552	578	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
Red Fluorescent Protein (RFP)	555	584	Expressed fluorescent protein	microscopy; flow cytometry
NovaFluor Yellow 590	563	594	Phiton-based DNA scaffold dye	flow cytometry
Alexa Fluor 568	579	603	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
PE-eFluor 610	565	606	Protein-based tandem dye	flow cytometry
Texas Red (and Texas Red-X)	595	613	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
NovaFluor Yellow 610	551	614	Phiton-based DNA scaffold dye	flow cytometry
Aluora 594	589	615	Small organic dye	microscopy
Alexa Fluor 594	590	618	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
NovaFluor Yellow 660	550	664	Phiton-based DNA scaffold dye	flow cytometry
NovaFluor Yellow 690	551	691	Phiton-based DNA scaffold dye	flow cytometry
NovaFluor Yellow 700	551	701	Phiton-based DNA scaffold dye	flow cytometry
NovaFluor Yellow 730	551	730	Phiton-based DNA scaffold dye	flow cytometry
NovaFluor Yellow 755	552	755	Phiton-based DNA scaffold dye	flow cytometry
PE-Cyanine7	569	780	Protein-based tandem dye	flow cytometry
NovaFluor Yellow 810	551	813	Phiton-based DNA scaffold dye	flow cytometry

Design tools that can help you get an excellent combination of dyes for your experiment

Red
(600-729 nm)

Fluorophore	Excitation	Emission	Chemical property	Platform
NovaFluor Red 660	637	658	Phiton-based DNA scaffold dye	flow cytometry
Allophycocyanin (APC)	651	660	Protein-based fluorophore	flow cytometry
Cy5	647	665	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
eFluor 660	651	668	Small organic dye	flow cytometry
Aluora 647	652	670	Small organic dye	microscopy
Alexa Fluor 647	650	671	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
NovaFluor Red 685	637	685	Phiton-based DNA scaffold dye	flow cytometry
Alexa Fluor 660	663	691	Small organic dye	flow cytometry
NovaFluor Red 700	637	701	Phiton-based DNA scaffold dye	flow cytometry
Alexa Fluor 680	681	704	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
Aluora 700	687	706	Small organic dye	microscopy
NovaFluor Red 710	637	713	Phiton-based DNA scaffold dye	flow cytometry
Alexa Fluor 700	696	719	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
NovaFluor Red 725	636	725	Phiton-based DNA scaffold dye	flow cytometry
NovaFluor Red 755	636	755	Phiton-based DNA scaffold dye	flow cytometry

Design tools that can help you get an excellent combination of dyes for your experiment

Near IR/IR
(730+ nm)

Fluorophore	Excitation	Emission	Chemical property	Platform
Alexa Fluor 750	752	776	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
Aluora 750	757	783	Small organic dye	microscopy
APC-eFluor 780	756	785	Protein-based tandem dye	flow cytometry

Design tools that can help you get an excellent combination of dyes for your experiment

Explore fluorophores based on peak emission wavelength

Fluorophore	Excitation	Emission	Chemical property	Platform
Brilliant Ultra Violet 395	347	399	Polymer dye	flow cytometry
Alexa Fluor 405	401	422	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
Brilliant Violet 421	406	423	Polymer dye	flow cytometry
Super Bright 436	413	431	Polymer dye	flow cytometry

Design tools that can help you get an excellent combination of dyes for your experiment

Fluorophore	Excitation	Emission	Chemical property	Platform
Alexa Fluor 350	343	441	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
eFluor 450	405	445	Small organic dye	flow cytometry
Pacific Blue	404	455	Small organic dye	flow cytometry
Coumarin and Coumarin Derivatives	387	470	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
Brilliant Violet 480	437	478	Polymer dye	flow cytometry
Cyan Fluorescent Protein (CFP)	435	485	Expressed fluorescent protein	microscopy; flow cytometry
Brilliant Ultra Violet 496	348	493	Tandem polymer dye	flow cytometry
Aluora 430	427	499	Small organic dye	microscopy

Design tools that can help you get an excellent combination of dyes for your experiment

Fluorophore	Excitation	Emission	Chemical property	Platform
eFluor 506	419	508	Small organic dye	flow cytometry
Green Fluorescent Protein (GFP)	488	510	Expressed fluorescent protein	microscopy; flow cytometry
Pacific Green	411	510	Small organic dye	flow cytometry
BODIPY FL	502	511	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
NovaFluor Blue 510	493	513	Phiton-based DNA scaffold dye	flow cytometry
Fluorescein (FITC)	498	517	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
Aluora 488	493	518	Small organic dye	microscopy
Alexa Fluor 488	499	520	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
Qdot 525 Probe	300	525	Quantum dot	microscopy; flow cytometry
Oregon Green 488	498	526	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
NovaFluor Blue 530	508	527	Phiton-based DNA scaffold dye	flow cytometry
Aluora 514	512	529	Small organic dye	microscopy

Design tools that can help you get an excellent combination of dyes for your experiment

Fluorophore	Excitation	Emission	Chemical property	Platform
Pacific Orange	400	551	Small organic dye	flow cytometry
Alexa Fluor 532	534	553	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
NovaFluor Blue 555	493	555	Phiton-based DNA scaffold dye	flow cytometry
Brilliant Ultra Violet 563	349	563	Tandem polymer dye	flow cytometry
Qdot 565 Probe	300	564	Quantum dot	microscopy; flow cytometry
Cy3	554	566	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
NovaFluor Yellow 570	551	566	Phiton-based DNA scaffold dye	flow cytometry
Aluora 555	553	567	Small organic dye	microscopy
Alexa Fluor 555	553	568	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
Alexa Fluor 561	556	569	Small organic dye	flow cytometry
Alexa Fluor 546	561	572	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
R-phycoerythrin (R-PE)	565	576	Protein-based fluorophore	flow cytometry
Tetramethylrhodamine (TRITC)	552	578	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
Red Fluorescent Protein (RFP)	555	584	Expressed fluorescent protein	microscopy; flow cytometry
NovaFluor Blue 585	493	586	Phiton-based DNA scaffold dye	flow cytometry
NovaFluor Yellow 590	563	594	Phiton-based DNA scaffold dye	flow cytometry

Design tools that can help you get an excellent combination of dyes for your experiment

Fluorophore	Excitation	Emission	Chemical property	Platform
Super Bright 600	414	601	Tandem polymer dye	flow cytometry
Alexa Fluor 568	579	603	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
Qdot 605 Probe	300	603	Quantum dot	microscopy; flow cytometry
Brilliant Violet 605	405	605	Tandem polymer dye	flow cytometry
PE-eFluor 610	565	606	Protein-based tandem dye	flow cytometry
Brilliant Ultra Violet 615	350	607	Tandem polymer dye	flow cytometry
Texas Red (and Texas Red-X)	595	613	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
NovaFluor Yellow 610	551	614	Phiton-based DNA scaffold dye	flow cytometry
Aluora 594	589	615	Small organic dye	microscopy
NovaFluor Blue 610-30S	508	615	Phiton-based DNA scaffold dye	flow cytometry
NovaFluor Blue 610-70S	492	616	Phiton-based DNA scaffold dye	flow cytometry
Alexa Fluor 594	590	618	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader

Design tools that can help you get an excellent combination of dyes for your experiment

Fluorophore	Excitation	Emission	Chemical property	Platform
Super Bright 645	414	645	Tandem polymer dye	flow cytometry
Brilliant Violet 650	407	646	Tandem polymer dye	flow cytometry
Qdot 655 Probe	300	654	Quantum dot	microscopy; flow cytometry
NovaFluor Red 660	637	658	Phiton-based DNA scaffold dye	flow cytometry
Brilliant Ultra Violet 661	349	659	Tandem polymer dye	flow cytometry
Allophycocyanin (APC)	651	660	Protein-based fluorophore	flow cytometry
NovaFluor Blue 660-40S	508	664	Phiton-based DNA scaffold dye	flow cytometry
NovaFluor Yellow 660	550	664	Phiton-based DNA scaffold dye	flow cytometry
Cy5	647	665	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
NovaFluor Blue 660-120S	492	665	Phiton-based DNA scaffold dye	flow cytometry
eFluor 660	651	668	Small organic dye	flow cytometry
Aluora 647	652	670	Small organic dye	microscopy
Alexa Fluor 647	650	671	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
PerCP-Cyanine5.5	489	679	Protein-based tandem dye	flow cytometry

Design tools that can help you get an excellent combination of dyes for your experiment

Fluorophore	Excitation	Emission	Chemical property	Platform
NovaFluor Red 685	637	685	Phiton-based DNA scaffold dye	flow cytometry
NovaFluor Blue 690	672	688	Phiton-based DNA scaffold dye	flow cytometry
Alexa Fluor 660	663	691	Small organic dye	flow cytometry
NovaFluor Yellow 690	551	691	Phiton-based DNA scaffold dye	flow cytometry
NovaFluor Red 700	637	701	Phiton-based DNA scaffold dye	flow cytometry
NovaFluor Yellow 700	551	701	Phiton-based DNA scaffold dye	flow cytometry
Qdot 705 Probe	300	702	Quantum dot	microscopy; flow cytometry
Super Bright 702	413	702	Tandem polymer dye	flow cytometry
Alexa Fluor 680	681	704	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
Aluora 700	687	706	Small organic dye	microscopy
PerCP-eFluor 710	482	708	Protein-based tandem dye	flow cytometry
Brilliant Violet 711	406	711	Tandem polymer dye	flow cytometry
NovaFluor Red 710	637	713	Phiton-based DNA scaffold dye	flow cytometry
Alexa Fluor 700	696	719	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
NovaFluor Red 725	636	725	Phiton-based DNA scaffold dye	flow cytometry

Design tools that can help you get an excellent combination of dyes for your experiment

Fluorophore	Excitation	Emission	Chemical property	Platform
NovaFluor Yellow 730	551	730	Phiton-based DNA scaffold dye	flow cytometry
Brilliant Ultra Violet 737	350	740	Tandem polymer dye	flow cytometry
NovaFluor Red 755	636	755	Phiton-based DNA scaffold dye	flow cytometry
NovaFluor Yellow 755	551	755	Phiton-based DNA scaffold dye	flow cytometry
Alexa Fluor 750	752	776	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
PE-Cyanine7	569	780	Protein-based tandem dye	flow cytometry
Super Bright 780	413	780	Tandem polymer dye	flow cytometry
Aluora 750	757	783	Small organic dye	microscopy
APC-eFluor 780	756	785	Protein-based tandem dye	flow cytometry
Brilliant Violet 786	406	788	Tandem polymer dye	flow cytometry
Qdot 800 Probe	300	792	Quantum dot	microscopy; flow cytometry
Brilliant Ultra Violet 805	349	804	Tandem polymer dye	flow cytometry

Design tools that can help you get an excellent combination of dyes for your experiment

Violet
(380–434 nm)

Fluorophore	Excitation	Emission	Chemical property	Platform
Brilliant Ultra Violet 395	347	399	Polymer dye	flow cytometry
Alexa Fluor 405	401	422	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
Brilliant Violet 421	406	423	Polymer dye	flow cytometry
Super Bright 436	413	431	Polymer dye	flow cytometry

Design tools that can help you get an excellent combination of dyes for your experiment

Blue
(435–499 nm)

Fluorophore	Excitation	Emission	Chemical property	Platform
Alexa Fluor 350	343	441	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
eFluor 450	405	445	Small organic dye	flow cytometry
Pacific Blue	404	455	Small organic dye	flow cytometry
Coumarin and Coumarin Derivatives	387	470	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
Brilliant Violet 480	437	478	Polymer dye	flow cytometry
Cyan Fluorescent Protein (CFP)	435	485	Expressed fluorescent protein	microscopy; flow cytometry
Brilliant Ultra Violet 496	348	493	Tandem polymer dye	flow cytometry
Aluora 430	427	499	Small organic dye	microscopy

Design tools that can help you get an excellent combination of dyes for your experiment

Green
(500–549 nm)

Fluorophore	Excitation	Emission	Chemical property	Platform
eFluor 506	419	508	Small organic dye	flow cytometry
Green Fluorescent Protein (GFP)	488	510	Expressed fluorescent protein	microscopy; flow cytometry
Pacific Green	411	510	Small organic dye	flow cytometry
BODIPY FL	502	511	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
NovaFluor Blue 510	493	513	Phiton-based DNA scaffold dye	flow cytometry
Fluorescein (FITC)	498	517	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
Aluora 488	493	518	Small organic dye	microscopy
Alexa Fluor 488	499	520	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
Qdot 525 Probe	300	525	Quantum dot	microscopy; flow cytometry
Oregon Green 488	498	526	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
NovaFluor Blue 530	508	527	Phiton-based DNA scaffold dye	flow cytometry
Aluora 514	512	529	Small organic dye	microscopy

Design tools that can help you get an excellent combination of dyes for your experiment

Yellow
(550–599 nm)

Fluorophore	Excitation	Emission	Chemical property	Platform
Pacific Orange	400	551	Small organic dye	flow cytometry
Alexa Fluor 532	534	553	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
NovaFluor Blue 555	493	555	Phiton-based DNA scaffold dye	flow cytometry
Brilliant Ultra Violet 563	349	563	Tandem polymer dye	flow cytometry
Qdot 565 Probe	300	564	Quantum dot	microscopy; flow cytometry
Cy3	554	566	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
NovaFluor Yellow 570	551	566	Phiton-based DNA scaffold dye	flow cytometry
Aluora 555	553	567	Small organic dye	microscopy
Alexa Fluor 555	553	568	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
Alexa Fluor 561	556	569	Small organic dye	flow cytometry
Alexa Fluor 546	561	572	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
R-phycoerythrin (R-PE)	565	576	Protein-based fluorophore	flow cytometry
Tetramethylrhodamine (TRITC)	552	578	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
Red Fluorescent Protein (RFP)	555	584	Expressed fluorescent protein	microscopy; flow cytometry
NovaFluor Blue 585	493	586	Phiton-based DNA scaffold dye	flow cytometry
NovaFluor Yellow 590	563	594	Phiton-based DNA scaffold dye	flow cytometry

Design tools that can help you get an excellent combination of dyes for your experiment

Orange
(600–629 nm)

Fluorophore	Excitation	Emission	Chemical property	Platform
Super Bright 600	414	601	Tandem polymer dye	flow cytometry
Alexa Fluor 568	579	603	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
Qdot 605 Probe	300	603	Quantum dot	microscopy; flow cytometry
Brilliant Violet 605	405	605	Tandem polymer dye	flow cytometry
PE-eFluor 610	565	606	Protein-based tandem dye	flow cytometry
Brilliant Ultra Violet 615	350	607	Tandem polymer dye	flow cytometry
Texas Red (and Texas Red-X)	595	613	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
NovaFluor Yellow 610	551	614	Phiton-based DNA scaffold dye	flow cytometry
Aluora 594	589	615	Small organic dye	microscopy
NovaFluor Blue 610-30S	508	615	Phiton-based DNA scaffold dye	flow cytometry
NovaFluor Blue 610-70S	492	616	Phiton-based DNA scaffold dye	flow cytometry
Alexa Fluor 594	590	618	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader

Design tools that can help you get an excellent combination of dyes for your experiment

Red
(630–679 nm)

Fluorophore	Excitation	Emission	Chemical property	Platform
Super Bright 645	414	645	Tandem polymer dye	flow cytometry
Brilliant Violet 650	407	646	Tandem polymer dye	flow cytometry
Qdot 655 Probe	300	654	Quantum dot	microscopy; flow cytometry
NovaFluor Red 660	637	658	Phiton-based DNA scaffold dye	flow cytometry
Brilliant Ultra Violet 661	349	659	Tandem polymer dye	flow cytometry
Allophycocyanin (APC)	651	660	Protein-based fluorophore	flow cytometry
NovaFluor Blue 660-40S	508	664	Phiton-based DNA scaffold dye	flow cytometry
NovaFluor Yellow 660	550	664	Phiton-based DNA scaffold dye	flow cytometry
Cy5	647	665	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
NovaFluor Blue 660-120S	492	665	Phiton-based DNA scaffold dye	flow cytometry
eFluor 660	651	668	Small organic dye	flow cytometry
Aluora 647	652	670	Small organic dye	microscopy
Alexa Fluor 647	650	671	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
PerCP-Cyanine5.5	489	679	Protein-based tandem dye	flow cytometry

Design tools that can help you get an excellent combination of dyes for your experiment

Far Red
(680–729 nm)

Fluorophore	Excitation	Emission	Chemical property	Platform
NovaFluor Red 685	637	685	Phiton-based DNA scaffold dye	flow cytometry
NovaFluor Blue 690	672	688	Phiton-based DNA scaffold dye	flow cytometry
Alexa Fluor 660	663	691	Small organic dye	flow cytometry
NovaFluor Yellow 690	551	691	Phiton-based DNA scaffold dye	flow cytometry
NovaFluor Red 700	637	701	Phiton-based DNA scaffold dye	flow cytometry
NovaFluor Yellow 700	551	701	Phiton-based DNA scaffold dye	flow cytometry
Qdot 705 Probe	300	702	Quantum dot	microscopy; flow cytometry
Super Bright 702	413	702	Tandem polymer dye	flow cytometry
Alexa Fluor 680	681	704	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
Aluora 700	687	706	Small organic dye	microscopy
PerCP-eFluor 710	482	708	Protein-based tandem dye	flow cytometry
Brilliant Violet 711	406	711	Tandem polymer dye	flow cytometry
NovaFluor Red 710	637	713	Phiton-based DNA scaffold dye	flow cytometry
Alexa Fluor 700	696	719	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
NovaFluor Red 725	636	725	Phiton-based DNA scaffold dye	flow cytometry

Design tools that can help you get an excellent combination of dyes for your experiment

Near IR/IR
(730+ nm)

Fluorophore	Excitation	Emission	Chemical property	Platform
NovaFluor Yellow 730	551	730	Phiton-based DNA scaffold dye	flow cytometry
Brilliant Ultra Violet 737	350	740	Tandem polymer dye	flow cytometry
NovaFluor Red 755	636	755	Phiton-based DNA scaffold dye	flow cytometry
NovaFluor Yellow 755	551	755	Phiton-based DNA scaffold dye	flow cytometry
Alexa Fluor 750	752	776	Small organic dye	microscopy; flow cytometry; fluorescence microplate reader
PE-Cyanine7	569	780	Protein-based tandem dye	flow cytometry
Super Bright 780	413	780	Tandem polymer dye	flow cytometry
Aluora 750	757	783	Small organic dye	microscopy
APC-eFluor 780	756	785	Protein-based tandem dye	flow cytometry
Brilliant Violet 786	406	788	Tandem polymer dye	flow cytometry
Qdot 800 Probe	300	792	Quantum dot	microscopy; flow cytometry
Brilliant Ultra Violet 805	349	804	Tandem polymer dye	flow cytometry

Design tools that can help you get an excellent combination of dyes for your experiment

Fluorophore brands

Alexa Fluor dyes

These high-performing dyes have intense fluorescence with conjugates available across the visible spectrum.

Brilliant Ultra Violet and Brilliant Violet polymer dyes

Brilliant Ultra Violet (BUV) and Brilliant Violet (BV) dyes use polymer technology to offer a broad range of conjugated antibodies for full use of UV and violet detector channels.

eFluor organic dyes

eFluor conjugated antibodies were developed for excellent performance in multiple applications, including multicolor flow cytometry and immunofluorescence microscopy.

NovaFluor dyes

NovaFluor dyes use the Phiton technology to create unique fluorescent signatures with minimal cross-excitation between laser lines resulting in lower need for compensation with increased resolution.

Super Bright polymer dyes

Super Bright dyes are comparable to Brilliant Violet dyes in terms of brightness while providing additional marker and clone options for violet-laser excitable antibody conjugates.

Qdot probes

Qdot probes offer bright fluorescence performance for single-excitation multicolor analysis with long-term photostability for live-cell imaging, fixability for post in vivo studies, and narrow emission spectra for low crosstalk.

Resources

Flow cytometry protocols handbook

Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.

Request the handbook

fluorophore-selection-guide-cover-736x736

Fluorophore and reagent selection guide for flow cytometry

A handy reference poster featuring the broad range of our dyes and labeling reagents available.

Request the guide

Imaging protocol handbook

Protocols that fit your needs in imaging ranging from sample and assay preparation to staining, labeling, and data analysis strategies.

Request the handbook

Cy™ is a trademark or registered trademark of GE Healthcare.

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.

For Research Use Only. Not for use in diagnostic procedures.