Spectraviewer image of NovaFluor Blue 690 dye

NovaFluor dyes, including NovaFluor Blue 690 dye, are built using Phiton technology and are compatible with both spectral flow cytometry as well as traditional flow cytometry. NovaFluor dyes exhibit narrow emission spectra and minimal cross-laser excitation, which helps reduce spectral spillover for better marker resolution. In addition, their unique spectral signatures can provide the opportunity to detect additional markers in flow cytometry panels by opening up previously unusable channels.

NovaFluor Blue 690 dye has a unique emission spectrum when excited by the 488nm laser, which fits in a spectral space that is currently only accessible using cross-laser excited tandem dyes, such as PerCP-Cyanine5.5. Replacing highly cross-laser excited dyes may potentially allow for the inclusion of an additional marker in a complex multicolor panel. NovaFluor Blue 690 dye should generally be paired with retain their fluorescence intensity and spectral signature when stored at 4°C.

Benefits of NovaFluor Blue 690 dye:

  • Narrow emission spectrum and minimal cross-laser excitation for better resolution
  • Should be paired with highly expressed antigens to minimize spillover
  • Can be used to replace cross-laser excited PerCP-Cyanine5.5
  • Produces highly stable fluorescence and is compatible with all buffers tested

Use NovaFluor dyes with CellBlox Blocking Buffer to reduce background and to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.

NovaFluor Blue 690 dye dashboard


Initial brightness
 
 
 
 
 
The NovaFluor Blue 690 dye, excited by the blue laser is optimized for use for cell-surface applications. Can be used for spectral and conventional flow cytometry applications. 

Photostability in buffer
 
 
 
 
 
 488695/40494690 
 
Laser line
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Excitation max


Emission max

 

NovaFluor Blue 690 dye products

We offer NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.

Antibody conjugates

Experimental data using NovaFluor Blue 690 dye products

Figure 1. Absorption and fluorescence emission spectra of NovaFluor Blue 690 dye.
 

Figure 2. Spectral signature of NovaFluor Blue 690 dye. Normal human peripheral blood cells stained with anti-CD4 antibodies (clone SK3) conjugated to NovaFluor Blue 690 dye were used for analysis. Data was acquired on a 5-laser Cytek Aurora system.
 

2-panel scatter plots of mouse splenocytes, unstained vs CD3-stained and CD19-stained vs CD3-stained

Figure 3. C57BL/6 mouse splenocytes were stained with CD3 monoclonal antibody, Super Bright 436 dye (Cat. No. 62-0037-42) only (left) or along with CD19 monoclonal antibody, NovaFluor Blue 690 dye (Cat. No. M004T03B09) (right). Viable cells were used for analysis, as determined by LIVE/DEAD Fixable Blue Dead Cell Stain Kit (Cat. No. L34962).
 

2-panel scatter plots of human PBMCs, unstained vs CD19-stained and CD27-stained vs CD19-stained
Figure 4. Normal human peripheral blood cells were stained with CD19 monoclonal antibody, eFluor 450 dye (Cat. No. 48-0199-42) only (left) or with CD27 monoclonal antibody, NovaFluor Blue 690 dye (Cat. No. H012T03B090) (right). Cells in the lymphocyte gate were used for analysis.