SpectraViewer image of Brilliant Violet 605 dye

Brilliant Violet™ dyes, including Brilliant Violet™ 605 (BV605), are a polymer dye-based technology and compatible with both spectral flow cytometry, as well as traditional flow cytometry. The violet laser has unique channels far from heavily occupied detectors allowing for larger panels.

BV605 is a tandem dye excited by the 405 nm violet laser and emits at 605 nm. This dye has a medium-high relative brightness and can be used for cell surface, intracellular, and nuclear staining.

When using two or more Super Bright, Brilliant Violet, Brilliant Ultra Violet™, or other polymer dye- conjugated antibodies in a staining panel, we recommend that you use Super Bright Complete Staining Buffer (Cat. No. SB-4401-42) or Brilliant Stain Buffer (Cat. No. 00-4409-42) to minimize any non- specific polymer interactions.

BV605 dye dashboard


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The violet laser-excitable BV605 dye is optimized for use both cell surface and intracellular flow applications. Can be used for spectral flow cytometry applications. 

Photostability in buffer
 
 
 
 
 
 405610/20407605 
 
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BV605 dye products

We offer BV605 dye conjugated to primary antibodies for use in flow cytometry.

Antibody conjugates

Brilliant Stain Buffer
Super Bright Staining Buffer

Experimental data using BV605 dye product

Figure 1. Excitation and emission of Brilliant Violet™ 605 dye (BV605).

Figure 2. Spectral signature of BV605 dye. Data acquired on a 5-laser Cytek Aurora and normal human peripheral blood cells stained with clone RPA-T8 (hCD8) conjugated to Brilliant Violet™ 605 dye (Cat. No. 406-0049-41) were used for analysis.

2-panel scatter plots of BV605 dye-stained Mouse IgG1 or BV605 dye-stained CD4 vs FITC CD3

Figure 3. Normal human peripheral blood cells were stained with CD3 Monoclonal Antibody, FITC (Cat. No.11-0037-42) and Mouse IgG1 kappa Isotype Control, Brilliant Violet™ 605 dye (Cat. No. 406-4714-81) (left) or CD4 Monoclonal Antibody, Brilliant Violet™ 605 dye (right). Viable cells in the lymphocyte gate were used for analysis, as determined by 7-AAD (Cat. No. 00-6993-50).

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.