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Brilliant Ultra Violet™ 615 Dye
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| 3 | 355 | 610/20, 595 LP | 350 | 607 | (in buffer) 3 | flow cytometry |
Invitrogen Brilliant Ultra Violet™ 615 (BUV615) is a tandem dye excited by the 355 nm UV laser and emits at 615 nm. This dye has a medium relative brightness and can be used for cell surface, intracellular, and nuclear staining. BUV615 may have some cross-laser excitation with the blue and yellow laser.
When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, we recommend using Super Bright Complete Staining Buffer or Brilliant Stain Buffer to minimize any non-specific polymer interactions.
We offer BUV615 dye conjugated to primary antibodies for use in flow cytometry.
Additional products
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Spectral signature of Brilliant Ultra Violet™ 615 dye. Data acquired on a 5-laser Cytek Aurora and normal human peripheral blood cells stained with CD3 Monoclonal Antibody (SK7), Brilliant Ultra Violet™ 615 were used for analysis.
Intracellular staining of mouse splenocytes using Brilliant Ultra Violet™ 615. C57BL/6 mouse splenocytes were unstimulated (left) or stimulated for 48 hours with CD3e Monoclonal Antibody, Functional Grade (right). Cells were then surface-stained with CD45R (B220) Monoclonal Antibody, FITC and stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set and protocol, with 1.0 µg of Ki-67 Monoclonal Antibody (SolA15), Brilliant Ultra Violet™ 615. Viable cells in the lymphocyte gate were used for analysis, as determined by LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit, for UV excitation.
Cell surface staining of human peripheral blood cells using Brilliant Ultra Violet™ 615. Normal human peripheral blood cells were stained with CD3 Monoclonal Antibody, FITC and Mouse IgG1 kappa Isotype Control, Brilliant Ultra Violet™ 615 (left) or CD8a Monoclonal Antibody, Brilliant Ultra Violet™ 615 (right). Viable cells in the lymphocyte gate were used for analysis, as determined by 7-AAD Viability Staining Solution.
Brilliant Ultra Violet™ dye background
Brilliant Ultra Violet dyes are a polymer dye-based technology and compatible with both spectral flow cytometry as well as traditional flow cytometry. The UV laser has unique channels far from heavily occupied detectors, allowing for larger panels.
Additional resources
Flow cytometry protocols handbook
Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.
