NovaFluor Yellow 590 excitation shown as dotted line and emission shown as solid yellow histogram
4561590/40563594(in buffer) 5
flow cytometry

Invitrogen NovaFluor Yellow 590 dye has a unique emission spectrum, which fits in between PE and PE-tandems such as PE-Dazzle 594, potentially allowing the detection of an additional marker in a complex multicolor panel. However, due to spectral spillover by PE and PE-tandems, an optimal panel design would replace PE and PE tandems with NovaFluor Yellow 570 and NovaFluor Yellow 610 dyes to reduce spillover into NovaFluor Yellow 590 dye channel. This dye should generally be paired with medium to highly expressed antigens to minimize spectral spillover in complex multicolor panels.

The macromolecule-based NovaFluor Yellow 590 dye produces highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C. Use NovaFluor dyes with CellBlox Plus Blocking Buffer to reduce background and to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.

We offer NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.

Search NovaFluor Yellow 590 primary antibodies

Benefits of NovaFluor Yellow 590 dye
  • Narrow emission spectrum and minimal cross-laser excitation for better resolution
  • Should be paired with medium to highly expressed antigens to minimize spillover
  • Can allow the detection of an additional marker between PE and PE-Tandems or NovaFluor Yellow 570 and NovaFluor Yellow 610
  • Produces highly stable fluorescence and is compatible with all buffers tested
Experimental data using NovaFluor Yellow 590 dye

Spectral signature of NovaFluor Yellow 590 dye. Normal human peripheral blood cells stained with CD4 Monoclonal Antibody (SK3 (SK-3)), NovaFluor Yellow 590 were used for analysis. Data was acquired on a 5-laser Cytek Aurora system.
 

Side scatter plot with unstained and CD4 NovaFluor Yellow 590 stained normal human PBMCs
Staining of human peripheral blood cells with NovaFluor Yellow 590 conjugated antibody. Normal human peripheral blood cells (PBMCs) were unstained (left) or stained with CD4 Monoclonal Antibody, NovaFluor Yellow 590 (right). Data was acquired in the YG2 channel on a 5-laser Cytek Aurora system, and singlet cells were used for analysis.

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