Applied Biosystems Real-Time PCR Instruments

Reactions per run: 96
Block format: 96
Color combinations (Multiplexing): Up to 3
Run time: ~40 min
Programmable volumes: 1-100 μL
Validated volumes: 5–100 μL
Automation compatible: No
Remote monitoring: Yes
On-Board memory: Yes, >1000 runs
Email notification: Yes
Assist with 21 CFR part 11 compliance: No
Sensitivity: Down to 1 copy
Dynamic range: Up to 10 logs**
Chemistry: All real-time PCR-based chemistries. Flexibility for chemistries with or without passive reference dye.
Applications available: Gene Expression, Genotyping, Copy Number Variation, Protein Detection, MicroRNA, Pathogen Detection
IVD platform: No
Temperature uniformity: ±0.4°C
Excitation source: Bright white LED
Filters/colors: 3
Size: W 27 cm x D 50 cm x H 40 cm

Reactions per run: 96
Block format: 96-Well 0.2-mL or 96-Well Fast 0.1-mL
Color combinations (Multiplexing): Up to 4
Run time: As fast as 30 min
Programmable volumes: 1-200 μL
Validated volumes: 10-100 µl (96-well 0.2mL), 10-30 µl (96-well 0.1mL)
Automation compatible: No
Remote monitoring: Yes
On-Board memory: Yes, >2,000 runs
Email notification: Yes
Assist with 21 CFR part 11 compliance: Security
Sensitivity: Down to 1 copy
Dynamic range: Up to 10 logs**
Chemistry: All real-time PCR-based chemistries. Flexibility for chemistries with or without passive reference dye.
Applications available: Gene Expression, Genotyping, Copy Number Variation, Protein Detection, MicroRNA, Pathogen Detection
IVD platform: No
Temperature uniformity: ± 0.4°C
Excitation source: OptiFlex™ System with white LED light
Filters/colors: 4 excitation filters (450–600 nm), 4 emission filters (500–640 nm)
Thermo Fisher Cloud: Direct connection
Wi-Fi connectivity: Yes
Size: W 27 cm x D 50 cm x H 40 cm

*Duplex capabilities with OpenArray® and multiplex capabilities for other formats.
**Up to 7 logs with OpenArray®.

Reactions per run: 96 or 384
Block format: 96-Well 0.2-mL, 96-Well Fast 0.1-mL, or 384-Well
Color combinations (Multiplexing): 96-well up to 21, 384-well up to 5
Run time: As fast as 30 min
Programmable volumes: 1-200 μL
Validated volumes: 10-100 µl (96-well 0.2mL), 10-30 µl (96-well 0.1mL), 5-20 µl (384-well)
Automation compatible: No
Remote monitoring: Yes
On-Board memory: Yes, >2,000 runs
Email notification: Yes
Assist with 21 CFR part 11 compliance: Security, auditing, e-signature package included
Sensitivity: Down to 1 copy
Dynamic range: Up to 10 logs**
Chemistry: All real-time PCR-based chemistries. Flexibility for chemistries with or without passive reference dye.
Applications available: Gene Expression, Genotyping, Copy Number Variation, Protein Detection, MicroRNA, Pathogen Detection
IVD platform: No
Temperature uniformity: ± 0.4°C
Excitation source: OptiFlex™ System with white LED light
Filters/colors: 96-well: 6 excitation filters (450–670 nm), 6 emission filters (500–720 nm), 384-well: 5 excitation filters (450–650 nm), 5 emission filters (500–700 nm)
Thermo Fisher Cloud: Direct connection
Wi-Fi connectivity: Yes
Size: W 27 cm x D 50 cm x H 40 cm

*Duplex capabilities with OpenArray® and multiplex capabilities for other formats.
**Up to 7 logs with OpenArray®.

Reactions per run: 96 or 384
Block format: 96, 96 Fast, 384, upgradeable to TaqMan™ Array (interchangeable)
Color combinations (Multiplexing):Up to 5
Run time: As fast as 30 min
Programmable volumes: 1-200 μL
Validated volumes: 5–100 μL
Automation compatible: Upgradable to automation capability
Remote monitoring: Yes
On-Board memory: Yes, >100 runs
Email notification: Yes
Assist with 21 CFR part 11 compliance: Security, auditing, e-signature package available separately
Sensitivity: Down to 1 copy
Dynamic range: Up to 10 logs**
Chemistry: All real-time PCR-based chemistries. Flexibility for chemistries with or without passive reference dye.
Applications available: Gene Expression, miRNA, Genopyting, Copy Number Variation, Protein Thermal Shift, HRM, Pathogen Detection
IVD platform: No
Temperature uniformity: ± 0.5°C
Excitation source: OptiFlex™ System with halogen lamp
Filters/colors: 5 excitation filters (450–650 nm), 5 emission filters (500–700 nm)
Thermo Fisher Cloud: connect to cloud through computer
Wi-Fi connectivity: No
Size: W 50.5 cm x D 67.2 cm x H 73.8 cm

*Duplex capabilities with OpenArray™ and multiplex capabilities for other formats.
**Up to 7 logs with OpenArray.

Reactions per run: 96
Block format: 96
Color combinations (Multiplexing): Up to 5
Run time: As fast as 30 min
Programmable volumes: 1-100 μL
Validated volumes: 5–100 μL
Automation compatible: Upgradable to automation capability
Remote monitoring: Yes
On-Board memory: Yes, >100 runs
Email notification: Yes
Assist with 21 CFR part 11 compliance: Upgradeable
Sensitivity: Down to 1 copy
Dynamic range: Up to 10 logs**
Chemistry: All real-time PCR-based chemistries. Flexibility for chemistries with or without passive reference dye.
Applications available:  Gene Expression, miRNA, Genotyping, Copy Number Variation, Protein Thermal Shift, HRM, Pathogen Detection
IVD platform: No
Temperature uniformity: ± 0.4°C
Excitation source: Bright white LED
Filters/colors: 5
Size: W 33.8 cm x D 52.5 cm x H 54.7 cm

Reactions per run: 96 or 384
Block format: 96, 96 Fast, 384, TaqMan™ Array (inter- changeable)
Color combinations (Multiplexing):Up to 21
Run time: As fast as 30 min
Programmable volumes: 1-200 μL
Validated volumes: 1–100 μL
Automation compatible: Yes
Remote monitoring: Yes
On-Board memory: Yes, >100 runs
Email notification: Yes
Assist with 21 CFR part 11 compliance: Security, auditing, e-signature package available separately
Sensitivity: Down to 1 copy
Dynamic range: Up to 10 logs**
Chemistry: All real-time PCR-based chemistries. Flexibility for chemistries with or without passive reference dye.
Applications available: Gene Expression, Genotyping, Copy Number Variation, HRM, Protein Thermal Shift, Protein Detection, MicroRNA, Pathogen Detection
IVD platform: Available in certain countries
Temperature uniformity: ± 0.5°C
Excitation source: OptiFlex™ System with halogen lamp
Filters/colors: 6 excitation filters (450–670 nm), 6 emission filters (500–720 nm)
Thermo Fisher Cloud: connect to cloud through computer
Wi-Fi connectivity: No
Size: W 50.5 cm x D 67.2 cm x H 73.8 cm

*Duplex capabilities with OpenArray™ and multiplex capabilities for other formats.
**Up to 7 logs with OpenArray.

Reactions per run: 96
Block format: 96
Color combinations (Multiplexing):Up to 6
Run time: As fast as 30 min
Programmable volumes: 1-100 μL
Validated volumes: 5–100 μL
Automation compatible: Yes
Remote monitoring: Yes
On-Board memory: Yes, >100 runs
Email notification: Yes
Assist with 21 CFR part 11 compliance: Security, auditing, e-signature package
Sensitivity: Down to 1 copy
Dynamic range: Up to 10 logs**
Chemistry: All real-time PCR-based chemistries. Flexibility for chemistries with or without passive reference dye.
Applications available: Gene Expression, miRNA, Genotyping, Copy Number Variation, Protein Thermal Shift, HRM, Pathogen Detection
IVD platform: No
Temperature uniformity: ± 0.4°C
Excitation source: Bright white LEDbr>Filters/colors: 6
Size: W 33.8 cm x D 52.5 cm x H 54.7 cm

*Duplex capabilities with OpenArray™ and multiplex capabilities for other formats. **Up to 7 logs with OpenArray.

Reactions per run: 96, 384, or 12,228
Block format: 96, 96 Fast, 384, TaqMan™ Array (interchangeable)
Color combinations (Multiplexing):Up to 21*
Run time: As fast as 30 min
Programmable volumes: 1-200 μL
Validated volumes: 33–100 μL
Automation compatible: Yes
Remote monitoring: Yes
On-Board memory: Yes, >100 runs
Email notification: Yes
Assist with 21 CFR part 11 compliance: Security, auditing, e-signature package available separately
Sensitivity: Down to 1 copy
Dynamic range: Up to 10 logs**
Chemistry: All real-time PCR-based chemistries. Flexibility for chemistries with or without passive reference dye.
Applications available: Gene Expression, Genotyping, Copy Number Variation, HRM, Protein Thermal Shift, Protein Detection, MicroRNA, Pathogen Detection, Digital PCR
IVD platform: No
Temperature uniformity: ± 0.5°C
Excitation source: OptiFlex™ System with white LED light
Filters/colors: 6 excitation filters (450–670 nm), 6 emission filters (500–720 nm)
Thermo Fisher Cloud: connect to cloud through computer
Wi-Fi connectivity: No
Size: W 50.5 cm x D 67.2 cm x H 73.8 cm

*Duplex capabilities with OpenArray™ and multiplex capabilities for other formats.
**Up to 7 logs with OpenArray.

Reactions per run: 96, 384, or 12,228
Block format: 20,000 partitions/chip
Colors:2 (endpoint detection)
Automation compatible: No
Assist with 21 CFR part 11 compliance: No
Thermo Fisher Cloud: connect to cloud through computer
Wi-Fi connectivity: No