iPSC Therapy Solutions

The first off-the-shelf reprogramming system manufactured in accordance with GMP requirements, offering high-efficiency Sendai delivery of reprogramming factors.

• Xeno-free workflow—generate iPSC lines from both fibroblasts and blood for clinical research
• Regulatory support—Traceability documentation, such as Certificate of Analysis and Certificate of Origin, available to support regulatory filing
• Designed with transition in mind—complimentary research-use CytoTune iPS 2.0 Sendai Reprogramming Kit helps facilitate translation to the clinic

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Gibco CTS StemScale PSC Suspension Medium is designed to support the expansion of pluripotent stem cells (PSCs) at scale in suspension culture. Its scalable, easy-to-use workflow and xeno-free formulation make it exceptional for translational and clinical applications.

• Maximal expansion capability—achieve up to 30% higher expansion than other PSC suspension media
• Scalable expansion for clinical applications—grow PSCs across multiple culture vessel formats and sizes, from small to large scale
• No cell strainer required—remove scale limitation, reduce contamination risk and hands-on time, and improve cell viability and yield

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Based on the widely cited Essential 8 Medium, Gibco CTS Essential 8 Medium was the first globally available human pluripotent stem cell (hPSC) culture medium with components not directly derived from animals.

• Supports long-term iPSC culture—Maintains iPSC marker expression and a normal karyotype over multiple passages
• Maintains differentiation potential—PSCs cultured in CTS Essential 8 Medium are able to generate cells of all three germ layers
• Helps reduce risk—Based on the same fully defined and highly consistent eight-component formulation as research-use Essential 8 Medium but without components directly of animal or human origin

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Gibco CTS KnockOut SR XenoFree Medium is a defined, xeno-free serum replacement based on the traditional KnockOut Serum Replacement, which has been cited in more than 2,000 publications and trusted for over 20 years.

• Supports pluripotency—Able to maintain the pluripotency, normal morphology, and karyotype of hPSCs
• Versatile—Can also be used for cryopreservation, derivation, and differentiation of iPSCs
• Designed for your applications—A first-to-market, xeno-free iPSC product

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Designed in the laboratory of James Thomson, Gibco CTS Vitronectin (VTN-N) Recombinant Human Protein is a defined matrix for feeder-free culture of pluripotent stem cells (PSCs) and is designed for clinical research.

• Animal origin-free—Reduced variability and contamination risk in PSC cultures.
• Optimized—Intended for use with CTS Essential 8 Medium
• Consistent—Supports transition from RUO Vitronectin

Gibco CTS Versene is a gentle non-enzymatic cell dissociation reagent for routine clump passaging of PSCs while maintaining viability over multiple passages.

• Maintain viability over multiple passages—PSCs passaged with this cGMP manufactured EDTA dissociation reagent maintain viability
• Designed with transition in mind—Same formulation as RUO Versene Solution but with additional characterization and quality control testing

Your choice of chemically defined human- and animal origin-free basal media for pluripotent stem cell culture. Based on traditional DMEM and DMEM/F12 formulations, these basal media are:

• Optimized—Lower osmolarity as compared to traditional formulations improve maintenance of undifferentiated iPSCs
• Versatile—Have been shown to support expansion and differentiation of a variety of stem cells, including iPSCs
• Tried and tested—Cited in many publications, often used with CTS KnockOut SR XenoFree supplement

Gibco CTS RevitaCell Supplement (100X) is an animal-origin-free, chemically defined supplement used with PSCs for post-thaw recovery or in combination with CTS Essential 8 Medium for single cell passaging.

• Improved cell survival—Provides high cell viability post-single cell passaging and post-thaw
• Contains higher specificity inhibitor—Includes a higher specificity ROCK inhibitor, coupled with antioxidants and free radical scavengers to minimize impact of stress on cells
• Offers consistency—Formulated using animal-origin free and chemically defined materials

The Gibco CTS PSC Cryopreservation Kit, which includes CTS PSC Cryomedium and CTS RevitaCell Supplement, minimizes the loss of cell viability and maximizes post-thaw recovery of PSCs.

• Provides efficient recovery—Routinely achieves higher viability than traditional methods of PSC cryopreservation
• Offers consistency—Chemically defined media maintains normal morphology, pluripotency, and karyotype of PSCs
• Reduces variability—Optimized reagents minimize the causes of genetic instability of PSCs

Gibco CTS Essential 6 Medium is a xeno-free, feeder-free medium which supports the spontaneous or directed differentiation of hPSCs and the reprogramming of somatic cells.

• Provides a flexible format—Suits different applications and various cell types
• Supports differentiation and reprogramming—Does not contain bFGF or TGFbeta
• Simplifies the transition from bench to clinic—Contains the same six essential components as RUO Essential 6 Medium, and with its cGMP manufacturing process, this formulation helps minimize variability

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Gibco B-27 Supplement has been the trusted standard for a variety of neuronal culture applications with citations in more than 11,000 publications. The CTS™ product offers proven serum-free supplementation for the growth, expansion, differentiation, and maintenance of PSC-derived cells.

• Applicable to numerous cell types - neural stem cells, neurons, and cardiomyocytes
• Three varieties available - full version, minus vitamin A, and minus insulin
• Equivalent performance - same performance as the trusted research‑use Gibco B‑27 Supplement XenoFree

Thermo Fisher Scientific offers stem cell growth factors, cytokines, and other stem cell-related recombinant proteins in the Gibco PeproTech portfolio to effectively maintain, expand, and differentiate stem cells into desired phenotypes. Learn more about how PeproTech and PeproGMP recombinant proteins can support your iPSC applications.

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