Mitochondria are membrane-bound organelles enclosed by a dual membrane. The outer membrane is relatively smooth; however, the inner membrane has many folds (cristae) that significantly increase the surface area of the inner membrane. These cristae are the site of ATP synthesis in which the energy from glucose is converted to ATP by oxidative phosphorylation. This process provides the cell’s principal energy source, giving it the power to carry out all of its varied functions. Mitochondria also contain their own ribosomes and DNA. While mitochondria primarily provide energy, they are also involved in cellular tasks like signaling, differentiation, apoptosis, and the control of the cell cycle and growth.

Mitochondrial marker antibodies detect proteins specific to the mitochondria and can aid in the study of the morphology and dynamics of the mitochondria. Mitochondrial marker antibodies can also help elucidate the role or roles a protein may play in a number of tasks that are centered in or influenced by the mitochondria. Invitrogen mitochondrial marker antibodies are designed to dependably detect the key mitochondrial targets. Each antibody is validated for use in various applications. Key mitochondrial marker targets include:

Mitochondrial marker antibody targets

Applications

Each Invitrogen mitochondrial marker antibody is validated for use in applications such as ELISA, western blot, flow cytometry, immunofluorescence, immunohistochemistry, immunocytochemistry, and immunoprecipitation.

Immunofluorescent analysis of Heat Shock Protein 60

Immunofluorescent analysis of Heat Shock Protein 60 (Hsp60) in U251 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock Protein 60 (Hsp60) monoclonal antibody (Cat. No. MA3-013) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Cat. No. 35503). Heat Shock Protein 60 (Hsp60) staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

Immunofluorescent analysis of ATP5A1

Immunofluorescent analysis of ATP5A1 in bovine pulmonary artery endothelial cells. Mitochondria were labeled using an anti-OxPhos Complex V subunit a, mouse IgG2b monoclonal antibody (Cat. No. 459240) prelabeled with the Zenon Alexa Fluor 555 Mouse IgG2b Labeling Kit (Cat. No. Z-25205). Tubulin was detected with an anti-a-tubulin mouse IgG2b monoclonal antibody prelabeled with the Zenon Alexa Fluor 488 Mouse IgG2b Labeling Kit (Cat. No. Z-25202), and The nucleus was stained with DAPI (Cat. No. D1306, D3571, D21490).

Resources

For Research Use Only. Not for use in diagnostic procedures.