Minimize the hassle, frustration, and sample-loss associated with a clogged instrument
Investigate difficult samples and large, clumpy cells, including cancer cells, cardiomyocytes, and adipocytes, which were previously not amenable to flow cytometry
The 2018 Attune NxT Flow Cytometer system makes multiparametric flow cytometry available to both new and experienced researchers. Experience the difference acoustic assisted hydrodynamic focusing technology brings to your workflow. Designed for faster experimental run times, this compact flow cytometer offers proven, rapid and accurate analysis for a broad range of sample types.
Efficient—up to 10X faster than traditional flow cytometric analyzers
Invitrogen Attune NxT 유세포 분석기는 최대 4개의 레이져와 최대 16개의 탐지 채널을 지원하는 벤치탑 유세포 분석기며 우수한 정밀도, 강력한 성능을 겸비하고 있습니다. Attune NxT 유세포 분석기는 유세포분석과 관련된 여러 장벽을 허물고 새로운 과학을 실현하려는 목표로 개발되었습니다. 이 목표에는 암 세포를 비롯하여 보다 다양한 샘플 유형 분석까지 가능한 유세포분석 기능이 포함되어 있습니다. 그 외에, Invitrogen Attune Autosampler를 추가로 사용하면 훨씬 빠른 고효율 다변수 스크리닝이 가능합니다.
시간 절약—데이터 품질 저하 없이 10배 빠른 속도 보장.
하루 종일 걸리는 작업 시간을 대폭 단축할 수 있습니다. Attune NxT 유세포 분석기를 연구에 사용해 보세요. 빨라진 획득률은 실험 시간을 줄이고 위대한 과학적 발견에 빠르게 도달할 수 있도록 도와줍니다. 이제 냉동고에서 샘플 품질이 저하되도록 두지 마시고, 지금 바로 분석하세요.
스트리밍 10 HD 영화와 동일한 전송 속도인, 데이터 손실 없는 강력한 35,000 events/se 획득과 1ml/min 유속 기능.
Acoustic focusing technology 기술 덕분에 기존 세포 분석기보다 최대 10배 빠른 속도, 세련된 fludic 디자인, 최적화된 광학, 향상된 데이터 전송.
Attune NxT Flow Cytometer는 경쟁사 장비 3대와 비교해 매우 짧은 시간에 이벤트 1,000,000개를 획득합니다. 데이터는 각 장비의 최대 샘플 속도(Attune NxT Cytometer의 경우 1,000µL/min)에서 유세포 분석기를 실행하여 확보한 프로토콜 시간을 나타냅니다.
Attune NxT Flow Cytometer 사용으로 높은 샘플 속도에서 데이터 변동 최소화.Jurkat 세포를 고정, propidium iodide으로 염색, RNase로 처리한 후 Attune NxT Flow Cytometer에서 농도 1x106cells/mL로 분석했습니다. 샘플 속도에 상관 없이, 심지어 가장 높은 샘플 속도인 1,000μL/min에서도 G0/G1과 G2/M 단계의 세포 변동계수(cv)가 변함 없이 유지됩니다.
막힘 방지 설계가 되어 있어 Attune NxT 유세포분석기는 종양 샘플, 심근세포, 다양한 부착 세포 등 여러 샘플에서 강력한 유세포분석을 할 수 있습니다.
장비 막힘에 따른 번거로움 및 불만, 샘플 손실 최소화
기존에 유세포분석기에서 처리 할 수 없었던 암세포, 심근 세포 및 지방세포를 포함한 크고 뭉치는 어려운 샘플 분석이 가능
유세포 분석기는 기존에는 두껍고 큰 샘플에서 제대로 작동하지 않았고, 자주 시스템 막힘 현상이 발생하여 샘플 손실, 프로젝트 지연, 상당한 장비 작동 모니터링 등 번거로움이 많았습니다. Attune NxT 세포분석기는 다른 유세포 분석기에서 분석이 불가능했던 두껍고 큰 세포 샘플분석이 가능합니다. 여기에는 종양 샘플, 심근세포, 지방세포 등 이전에는 제외되었던 다양한 유형이 포함되어 이제는 매우 다양한 샘플 유형에서 고효율 통계 분석이 가능하게 되었습니다.
연구 요구사항 증가에 따른 기능 확장
Invitrogen™ Attune™ NxT 유세포 분석기는 유세포 분석에 사용되는 가장 일반적인 형광발색단과 형광 단백질을 수용할 수 있도록 설계되어 현재 사용 중인 패널에 잘 맞습니다. 561nm 레이저 옵션을 사용해 다양한 형광 단백질을 검출할 수 있습니다. 현재 시스템을 구성하여 사용하고 있든 나중에 업그레이드하든 관계 없이 연구자와 연구 필요에 맞게 Attune NxT 유세포 분석기를 업그레이드할 수 있습니다.
샘플 속도를 획기적으로 높이는 음향 집속(Acoustic focusing)으로 샘플 준비가 간편해지고, 프로토콜 시간이 단축되며, 세포 유실이 최소화됩니다. 이 기능은 뇌척수액(CSF)과 줄기세포 준비처럼 본래 농도가 낮은 샘플과 전혈을 사용하는, 세척과 용해가 필요 없는 immunophenotyping 프로토콜에서 유용합니다.
Attune NxT 유세포 분석기는 최소 처리된 샘플에서 immunophenotyping 분석을 실행할 수 있어 10단계 샘플 준비 프로토콜이 3단계로 간소화됩니다.
더 많은 데이터, 더욱 상세한 정보, 더 높은 처리량 확보는 사용자의 실험 설계와 사용 중인 장비 역량에 따라 좌우됩니다. 따라서 다음 번 결과 도출에도 영향을 미칩니다. Attune NxT 유세포 분석기는 더욱 복잡한 실험에서 요구하는 사항을 충족할 수 있도록 설계되어 안정적인 데이터를 매일 계속 제공할 뿐 아니라 사용자의 필요에 따라 업그레이드할 수 있습니다.
Emission filters: Up to 14 color channels with wavelength-tuned photomultiplier tubes (PMTs); user-changeable, keyed filters
Laser separation: 150 μm
Optical alignment: Fixed alignment with prealigned welded fiber; no user maintenance require
Onboard thermal-electric cooler: No warm-up delay; fiber isn't affected by on/off
Simmer mode: Instant on/off reduces usage and/or aging by 10x; only keep it "on" when acquiring samples; reports hours of usage
––Flat top specified at the flow cell; CV <3% over width of flat top ––Field upgradable
Fluidics
Flow cell: Quartz cuvette gel coupled to 1.2 numerical aperture (NA) collection lens, 200 x 200 μm
Sample analysis volume: 20 μL to 4 mL
Custom sample flow rates: 12.5–1,000 μL/min
Sample delivery: Positive-displacement syringe pump for volumetric analysis
Sample tubes: Accommodates tubes from 17 x 100 mm to 8.5 x 45 mm
Fluid-level sensing: Active
Standard fluid reservoirs: 1.8 L focusing fluid tank, 1.8 L waste tank, 175 mL shutdown solution tank, and 175 mL wash solution tank
Fluid storage: All fluids stored within instrument
Extended fluidics option: Configuration for 10 L fluid
Nominal fluid consumption: 1.8 L/day
Automated maintenance cycles: ≤15 min startup and shutdown—deep clean, sanitize, and debubble modes
Performance
Fluorescence sensitivity: ≤80 MESF for FITC, ≤30 MESF for PE, ≤70 MESF for APC
Fluorescence resolution: CV <3% for the singlet peak of propidium iodide–stained chicken erythrocyte nuclei (CEN)
Data acquisition rate: Up to 35,000 events/sec, 34 parameters, based on a 10% coincidence rate per Poisson statistics
Maximum electronic speed: 65,000 events/sec with all parameters
Carryover: Single-tube format: <1%
Forward and side scatter sensitivity: Able to discriminate platelets from noise
Forward and side scatter resolution: Optimized to resolve lymphocytes, monocytes, and granulocytes in lysed whole blood
Forward scatter: Photodiode detector with 488/10 nm bandpass filter
Side scatter: PMT with default 488/10 nm bandpass filter; optional 405/10 nm bandpass filter
Fluorescent detectors: 14 individual detectors
Electronic pulse: Measured area, height and width pulse for all detectors
Violet side scatter resolution: Can be configured for violet side scatter to better resolve particles from noise
Minimum particle size: 0.2 μm on side scatter using submicron bead calibration kit from Bangs Laboratories
Software
Compensation: Full matrix—automated and manual modes, on-plot compensation tools for fine adjustment; use of tubes and wells
Flow rate: Precise flow rate control via software; no hardware adjustments
Live streaming: Live update of statistics during acquisition of events up to 35,000 events/sec
Overlays: Comparative analysis between samples; 3D view
Sample recovery: System able to return unused samples
Concentration: Direct concentration measurement without use of counting beads
Software layout: Fully customizable for each user account
Bubble detection technology: Stops automated run to preserve sample integrity
Maximum single-event file: 20 million with option to append
Heat map: Set up for definition of plate layout; screening view for analysis for tubes and plates
Threshold: Up to 4 individual thresholds with user option to apply Boolean logic
Gating: Hierarchal gating with the ability to derive gates
Smartgate labeling: Option to annotate quad gate names based on fluorophore and target names
Voltage: User adjustable
Window extensions: User adjustable
Area scaling factor (ASF): User adjustable
Acquisition settings: Documented in FCS files and maintained upon import
Templates: Create from existing experiments—instrument settings, workspaces, run protocols, heat map settings, and compensation settings optimized and defined previously
Tube-to-plate conversion: One-click transition from tubes to plates and vice versa; no disassembly, no additional QC, no reboot required for conversion between plates and tubes
Graphics resolution: Publication-quality images; support for TIF, PNG, BMP, JPG, GIF, and EMF; quickly copy and paste plots to any external application (e.g., Microsoft™ PowerPoint™ software)
User account administration: Administrative creation of individual user accounts with designated roles, advanced setting permissions, management of individual accounts, user time tracking, and sample count
Quality and regulatory
Instrument tracking: Automated daily baseline and performance test with Levey-Jennings plots
Warranty: 1 year
Production verification testing: Each instrument is tested and verified for assembly integrity and performance to specifications
Quality management system: Manufacturing standards comply with the requirements of ISO 13485:2003
Robust install specifications: Units installed by engineer; preplanning checklist, delivery, installation, and performance validation comply with standardized procedure
Monitor: 23-inch flat panel (1,920 x 1,200 resolution); dual-monitor capability
Computer: Minitower desktop
Operating system: Windows™ 7 64-bit
FCS format: FCS 3.1, 3.0
Processor: Intel Core™ i7 processor
RAM: 16 GB
Hard drives: 80 GB or larger and 250 GB RAID-compatible hard drives
Installation requirements
Electrical requirements: 100–240 VAC, 50/60 Hz, <150 W. Thermo Fisher Scientific certifies that the Attune NxT Flow Cytometer conforms to relevant directives to bear the CE mark. The instrument also conforms to the UL and CAN/CSA general requirements (61010.1). The Attune NxT Flow Cytometer is a Class I laser product per CDRH regulations and EN/IEC 60825.
Heat dissipation: <150 W
Temperature operating ranges: 15–30°C (59–86°F)
Operating humidity: 10–90%, noncondensing
Audible noise: <65 dBA at 1.0 m
Instrument size (H x W x D): ~40 x 58 x 43 cm (16 x 23 x 17 in.), including fluid bottles
Attune Acoustic Focusing Cytometer의 고민감도 적용 발표자: 빌 텔포드(Bill Telford), CYTO 2012에서 실험 이식 및 면역학 부문(Experimental Transplantation and Immunonology Branch)의 FACS Core Facility 책임자.
Attune® Acoustic Focusing Cytometer를 사용한 말라리아 연구 발표자: 그레이스 초이노스키(Grace Chojnowski), CYTO 2012에서 Queensland Institute of Medical Research의 유세포 분석 및 영상촬영 시설(Flow Cytometry and Imaging Facility) 관리자
Emission filters: Up to 14 color channels with wavelength-tuned photomultiplier tubes (PMTs); user-changeable, keyed filters
Laser separation: 150 μm
Optical alignment: Fixed alignment with prealigned welded fiber; no user maintenance require
Onboard thermal-electric cooler: No warm-up delay; fiber isn't affected by on/off
Simmer mode: Instant on/off reduces usage and/or aging by 10x; only keep it "on" when acquiring samples; reports hours of usage
––Flat top specified at the flow cell; CV <3% over width of flat top ––Field upgradable
Fluidics
Flow cell: Quartz cuvette gel coupled to 1.2 numerical aperture (NA) collection lens, 200 x 200 μm
Sample analysis volume: 20 μL to 4 mL
Custom sample flow rates: 12.5–1,000 μL/min
Sample delivery: Positive-displacement syringe pump for volumetric analysis
Sample tubes: Accommodates tubes from 17 x 100 mm to 8.5 x 45 mm
Fluid-level sensing: Active
Standard fluid reservoirs: 1.8 L focusing fluid tank, 1.8 L waste tank, 175 mL shutdown solution tank, and 175 mL wash solution tank
Fluid storage: All fluids stored within instrument
Extended fluidics option: Configuration for 10 L fluid
Nominal fluid consumption: 1.8 L/day
Automated maintenance cycles: ≤15 min startup and shutdown—deep clean, sanitize, and debubble modes
Performance
Fluorescence sensitivity: ≤80 MESF for FITC, ≤30 MESF for PE, ≤70 MESF for APC
Fluorescence resolution: CV <3% for the singlet peak of propidium iodide–stained chicken erythrocyte nuclei (CEN)
Data acquisition rate: Up to 35,000 events/sec, 34 parameters, based on a 10% coincidence rate per Poisson statistics
Maximum electronic speed: 65,000 events/sec with all parameters
Carryover: Single-tube format: <1%
Forward and side scatter sensitivity: Able to discriminate platelets from noise
Forward and side scatter resolution: Optimized to resolve lymphocytes, monocytes, and granulocytes in lysed whole blood
Forward scatter: Photodiode detector with 488/10 nm bandpass filter
Side scatter: PMT with default 488/10 nm bandpass filter; optional 405/10 nm bandpass filter
Fluorescent detectors: 14 individual detectors
Electronic pulse: Measured area, height and width pulse for all detectors
Violet side scatter resolution: Can be configured for violet side scatter to better resolve particles from noise
Minimum particle size: 0.2 μm on side scatter using submicron bead calibration kit from Bangs Laboratories
Software
Compensation: Full matrix—automated and manual modes, on-plot compensation tools for fine adjustment; use of tubes and wells
Flow rate: Precise flow rate control via software; no hardware adjustments
Live streaming: Live update of statistics during acquisition of events up to 35,000 events/sec
Overlays: Comparative analysis between samples; 3D view
Sample recovery: System able to return unused samples
Concentration: Direct concentration measurement without use of counting beads
Software layout: Fully customizable for each user account
Bubble detection technology: Stops automated run to preserve sample integrity
Maximum single-event file: 20 million with option to append
Heat map: Set up for definition of plate layout; screening view for analysis for tubes and plates
Threshold: Up to 4 individual thresholds with user option to apply Boolean logic
Gating: Hierarchal gating with the ability to derive gates
Smartgate labeling: Option to annotate quad gate names based on fluorophore and target names
Voltage: User adjustable
Window extensions: User adjustable
Area scaling factor (ASF): User adjustable
Acquisition settings: Documented in FCS files and maintained upon import
Templates: Create from existing experiments—instrument settings, workspaces, run protocols, heat map settings, and compensation settings optimized and defined previously
Tube-to-plate conversion: One-click transition from tubes to plates and vice versa; no disassembly, no additional QC, no reboot required for conversion between plates and tubes
Graphics resolution: Publication-quality images; support for TIF, PNG, BMP, JPG, GIF, and EMF; quickly copy and paste plots to any external application (e.g., Microsoft™ PowerPoint™ software)
User account administration: Administrative creation of individual user accounts with designated roles, advanced setting permissions, management of individual accounts, user time tracking, and sample count
Quality and regulatory
Instrument tracking: Automated daily baseline and performance test with Levey-Jennings plots
Warranty: 1 year
Production verification testing: Each instrument is tested and verified for assembly integrity and performance to specifications
Quality management system: Manufacturing standards comply with the requirements of ISO 13485:2003
Robust install specifications: Units installed by engineer; preplanning checklist, delivery, installation, and performance validation comply with standardized procedure
Monitor: 23-inch flat panel (1,920 x 1,200 resolution); dual-monitor capability
Computer: Minitower desktop
Operating system: Windows™ 7 64-bit
FCS format: FCS 3.1, 3.0
Processor: Intel Core™ i7 processor
RAM: 16 GB
Hard drives: 80 GB or larger and 250 GB RAID-compatible hard drives
Installation requirements
Electrical requirements: 100–240 VAC, 50/60 Hz, <150 W. Thermo Fisher Scientific certifies that the Attune NxT Flow Cytometer conforms to relevant directives to bear the CE mark. The instrument also conforms to the UL and CAN/CSA general requirements (61010.1). The Attune NxT Flow Cytometer is a Class I laser product per CDRH regulations and EN/IEC 60825.
Heat dissipation: <150 W
Temperature operating ranges: 15–30°C (59–86°F)
Operating humidity: 10–90%, noncondensing
Audible noise: <65 dBA at 1.0 m
Instrument size (H x W x D): ~40 x 58 x 43 cm (16 x 23 x 17 in.), including fluid bottles
Attune Acoustic Focusing Cytometer의 고민감도 적용 발표자: 빌 텔포드(Bill Telford), CYTO 2012에서 실험 이식 및 면역학 부문(Experimental Transplantation and Immunonology Branch)의 FACS Core Facility 책임자.
Attune® Acoustic Focusing Cytometer를 사용한 말라리아 연구 발표자: 그레이스 초이노스키(Grace Chojnowski), CYTO 2012에서 Queensland Institute of Medical Research의 유세포 분석 및 영상촬영 시설(Flow Cytometry and Imaging Facility) 관리자
3D Demo: Explore the Attune NxT Flow Cytometer and Autosampler
A software tool for exploring the hardware, software, optics and fluidics of the Attune NxT Flow Cytometer. Hot spots throughout the tool feature data, researcher opinions, and technical specifications. Mouse operation enables rotation, zoom, and measurement. Available in 8 languages.
This online tool guides you through flow cytometry panel design, providing a simplified, customizable experience to fit your flow cytometry panel design needs.
Use these quick guides to find antibodies and other conjugates of a broad range of fluorescent dyes, fluorescent proteins, Qdot probes, and phycobiliproteins.
This online tool allows researchers to assess the spectral compatibility of dyes and probes in the course of designing experiments that utilize fluorescence detection techniques.