Flow cytometry analysis of a sample with dead, apoptotic, and live cells

There are many different apoptosis assays for flow cytometry because cell death cascades are complex and dynamic, underscoring the importance of a multi-parametric approach to the assessment of apoptosis. Since no single parameter defines programmed cell death, a combination of techniques is recommended for flow cytometry apoptosis detection.

Flow cytometry apoptosis detection assays

We offer a wide variety of apoptosis assays for flow cytometry that detect changes in the plasma and mitochondrial membranes, caspase activity, nuclear condensation, and fragmentation as possible indicators of apoptosis. Mix and match our standalone reagents or choose from our selection of multi-parametric apoptosis assay kits that are designed to selectively differentiate apoptotic cells from living and necrotic cells in a single cell population.

Selection Guide: search apoptosis assays for flow cytometry

Select flow cytometry apoptosis detection reagents based on the laser excitation source and common emission filters.

UV laser (~350 nm) reagents

Emission filter379/28 nm440/40 nm
Annexin V conjugates 
Membrane permeability & chromatin condensation 

Yellow laser (561 nm) reagents

UV laser (~350 nm) reagents

Emission filter379/28 nm440/40 nm
Annexin V conjugates 
Membrane permeability & chromatin condensation 

Yellow laser (561 nm) reagents


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Resources

Fluorophore and reagent selection guide for flow cytometry

Download Flow Cytometry Protocols Handbook

Spectral Flow Cytometry Fundamentals

Invitrogen eBioscience Resources—Selection guides, Best Protocols, product performance and more.

Intracellular Staining for Flow Cytometry How-To Video—for detecting cytokines and intranuclear markers.

Flow Cytometry Learning Center—Access flow cytometry educational resources for better experiment planning and execution.

Flow Cytometry Panel Builder—Design your flow cytometry panel with this online tool for a simplified, customizable experience to fit your needs.

5 Steps Resources

Support

Flow Cytometry Support Center—Find technical support recommendations for your flow cytometry workflows, including tips for experimental setup and in-depth troubleshooting help.

Flow Cytometry Panel Design Support—Work with one of our technical sales specialists to discuss your experimental needs and guide you through the process.

Flow Cytometry Protocols

Flow Cytometry Application Notes and Posters

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