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Cell viability assays for flow cytometry are reliable methods to distinguish live and dead cell populations. Removing dead and dying cells from your flow cytometry data is critical to enable the accuracy of your results and analysis. Dead cells often give false positive results, as a compromised cell membrane allows for internalization and non-specific binding of many reagents.
Flow cytometry viability stains are available for use with fixable and non-fixable cell samples in a broad range of color options that are compatible with most instrument configurations.
| LIVE/DEAD fixable viability dyes | Cell viability assays | LIVE/DEAD cell viability assays | Bacterial viability and vitality assays | Yeast viability and vitality assays | |
|---|---|---|---|---|---|
| Amine-reactive dyes | Nucleic acid binding dyes | Enzymatic substrates | Mammalian kits Bacterial kits Yeast kits | Bacterial kits | Yeast kits |
| Fixable | Non-fixable | Non-fixable | Non-fixable | Non-fixable; fixable | Non-fixable |
| Cell-impermeant | Cell-impermeant | Cell-permeant | Cell-impermeant; cell-permeant | Cell-impermeant; Cell-permeant | Cell-impermeant; Cell-permeant |
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| Dead cells | Dead cells | Live cells | Live cells; Dead cells | Live cells; Dead cells | Live cells; Dead cells |
| UV—Near IR | UV, Violet, Blue, Yellow, Red | UV, Violet, Blue, Yellow | Violet, Blue, Yellow | Violet, Blue, Yellow | Blue, Yellow |
See also:
Flow Cytometry Capabilities Guide
Download a copy of our Flow Cytometry Capabilities Guide and learn about our comprehensive suite of flow cytometry solutions developed to deliver you high-performance results and save you time.
Resources
Fluorophore and reagent selection guide for flow cytometry
Download Flow Cytometry Protocols Handbook
Spectral Flow Cytometry Fundamentals
Invitrogen eBioscience Resources—Selection guides, Best Protocols, product performance and more.
Intracellular Staining for Flow Cytometry How-To Video—for detecting cytokines and intranuclear markers.
Flow Cytometry Learning Center—Access flow cytometry educational resources for better experiment planning and execution.
Flow Cytometry Panel Builder—Design your flow cytometry panel with this online tool for a simplified, customizable experience to fit your needs.
5 Steps Resources
Support
Flow Cytometry Support Center—Find technical support recommendations for your flow cytometry workflows, including tips for experimental setup and in-depth troubleshooting help.
Flow Cytometry Panel Design Support—Work with one of our technical sales specialists to discuss your experimental needs and guide you through the process.