NovaFluor Blue 660-40S excitation shown as dotted line and emission shown as solid red histogram
2488660/20508664(in buffer) 5
flow cytometry

Invitrogen NovaFluor Blue 660 dyes are available with two different levels of brightness—NovaFluor Blue 660-40S and NovaFluor Blue 660-120S—that allow differential matching of the dye brightness to the antigen density or abundance without panel redesign. NovaFluor Blue 660-40S dye is dimmer than NovaFluor 660-120S dye. NovaFluor Blue 660-40S dye can be used to replace PE-tandem dyes, such as PE-Cy5 and PE/Fire™ 640, using the 488 nm blue laser line for excitation. Because PE-tandem dyes are cross-excited by both blue and yellow-green lasers, the replacement of these dyes with NovaFluor Blue 660-40S dye can allow detection of an additional marker labeled with, for example, NovaFluor Yellow 660 dye.

The macromolecule-based NovaFluor Blue 660-40S dye produces highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C. Use NovaFluor dyes with CellBlox Plus Blocking Buffer to reduce background and to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.

We offer NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.

Search NovaFluor Blue 660-40S primary antibodies

Benefits of NovaFluor Blue 660-40S dye
  • Narrow emission spectrum and minimal cross-laser excitation for better resolution
  • Lower intensity allows it to be paired with highly expressed antigens
  • Can serve as a replacement for PE-tandem dyes using the blue laser, with reduced cross-laser excitation and fluorescent spillover
  • Single PE-tandems can be replaced by the NovaFluor Blue 660-40S and NovaFluor Yellow 660 dyes to allow detection of an additional marker in a flow cytometry panel
  • NovaFluor Blue 660-40S and NovaFluor Blue 660-120S can be used together and unmixed using a spectral flow cytometer
  • Produces highly stable fluorescence and is compatible with all buffers tested
Experimental data using NovaFluor Blue 660-40S dye

Spectral signature of NovaFluor Blue 660-40S dye. Normal human peripheral blood cells stained with CD4 Monoclonal Antibody (SK3 (SK-3)), NovaFluor Blue 660-40S were used for analysis. Data was acquired on a 5-laser Cytek Aurora system.

Staining of human peripheral blood cells with NovaFluor Blue 660-40S conjugated antibody. Normal human peripheral blood cells (PBMCs) were unstained (left) or stained with CD3 Monoclonal Antibody, NovaFluor Blue 660-40S (right). All cells were co-stained with CD19 Monoclonal Antibody, eFluor 450. Total viable cells in the lymphocyte gate were used for analysis, as determined by LIVE/DEAD Fixable Blue Dead Cell Stain Kit, for UV excitation. Data was acquired on a 5-laser Cytek Aurora and unmixed with autofluorescence extraction.

Additional resources

Cy™ is a trademark or registered trademark of GE Healthcare.

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.

For Research Use Only. Not for use in diagnostic procedures.

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and is used under license.

For Research Use Only. Not for use in diagnostic procedures.