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Isolate and purify high-quality genomic DNA from a wide variety of sample types, including tissue, cells, blood, serum, plants, and forensic samples. Whether you prefer organic reagents, filter columns, or magnetic beads, our DNA purification products are designed for sensitive, scalable extraction and are compatible with a range of downstream applications.
Viral DNA extraction poses unique challenges for good recovery and detection sensitivity. Capture and lysis of virus particles from very dilute solutions or cell-free samples is the first hurdle. We have developed DNA purification products that are optimized to provide maximum viral DNA yield, purity, and integrity from a broad range of sample types in several format options.
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High-throughput recovery low abundance of viral DNA | Viral DNA purification from a variety of sample types | Consistent recovery of low abundance of viral DNA | |
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![]() PureLink Pro 96 Viral RNA/DNA Purification Kit | ![]() MagMAX Pathogen RNA/DNA Kit | ![]() PureLink Viral RNA/DNA Mini Kit | |
Starting material | 200 µL cell-free sample | 50–300 µL | 500 µL cell-free sample |
Sample type | Plasma, serum, cerebrospinal fluid | Plasma, serum, whole blood, swabs, feces, ear notches, semen, oral fluid, InPouch cultures | Plasma, serum, cerebrospinal fluid |
Protocol time | 30 mins | 45 mins | 45 mins |
Isolation method | Silica plate | Scalable, flexible format with magnetic beads | Fast, convenient silica column |
High-throughput compatible | |||
Number of preps | 384 | 480 | 50 |
Obtaining high-quality, intact DNA is often the first and most critical step in many fundamental molecular biology applications, such as DNA cloning, sequencing, PCR, and electrophoresis. Isolating intact total DNA from tissue samples varies in difficulty with the physical and biochemical nature of the tissue. For example, many DNA extraction methods will work equally well for tissues such as liver, but fibrous tissues such as heart, fatty tissues such as brain, and nuclease-rich tissues like spleen present challenges for DNA isolation. We offer a wide range of DNA extraction from tissue kits, plus the support you may need to get high yields of pure, intact DNA from almost any type of tissue.
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Process the largest amount of tissue | Fast isolation of gDNA from a variety of samples | High-yield, high-purity gDNA in a plate format | Rapid and automated extraction of DNA | |
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![]() DNAzol Reagent | ![]() PureLink Genomic DNA Mini Kit | ![]() PureLink Pro 96 Genomic DNA Purification Kit | ![]() MagMAX-96 DNA Multi-Sample Kit | |
Tissue starting material | Up to 50 mg | Up to 25 mg | Up to 25 mg | Up to 25–50 mg |
Yield | Up to 250 µg | 5–10 µg | 5–10 µg | 10–80 µg |
Isolation method | Organic extraction | Silica membrane | Filter plate | Magnetic beads |
High-throughput compatible | ||||
Compatible applications | Cloning, qPCR, sequencing | Cloning, qPCR, sequencing, genotyping | Cloning, qPCR, sequencing, genotyping | Cloning, qPCR, sequencing, genotyping |
Prep time | 10–30 mins | 15 mins | 35 mins | 45 mins |
Prep size | 100 mL | 50 and 250 preps | 4 x 96 preps | 50, 96, 500, and 2,500 preps |
DNA extraction from cells is one of the most widely used molecular biology techniques. This has traditionally been a highly repetitive and labor-intensive procedure, requiring significant time investment, and often involving the use of toxic organic chemicals. We have developed purification products that are optimized to provide DNA of maximum yield, purity, and integrity from cultured cells, including cell suspensions using several technology formats; organic extraction, silica membranes, and magnetic beads. These options provide users with flexibility, throughput, and choice along both the product and price continuum.
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Process the most cells | Fast isolation of gDNA from cells | High-yield, high-purity gDNA in a plate format | Rapid and automated extraction of DNA | |
![]() DNAzol Reagent | ![]() PureLink Genomic DNA Mini Kit | ![]() PureLink Pro 96 Genomic DNA Purification Kit | ![]() MagMAX-96 DNA Multi-Sample Kit | |
Cell input | Up to 3 x 107 cells | Up to 5 x 106 cells | Up to 5 x 106 cells | Up to 5 x 106 cells |
Yield | Up to 70 µg | Up to 10 µg | Up to 10 µg | 15–30 µg |
Isolation method | Organic extraction | Silica membrane | Filter plate | Magnetic beads |
High-throughput compatible | ||||
Compatible applications | Cloning, qPCR, sequencing | Cloning, qPCR, sequencing, genotyping | Cloning, qPCR, sequencing, genotyping | Cloning, qPCR, sequencing, genotyping |
Prep time | 10-30 min | 15 min | 35 min | 45 min |
Prep size | 100 mL | 50 and 250 preps | 4 x 96 preps | 50, 96, 500, and 2,500 preps |
Figure 1. Higher, more concentrated yields achieved with the Invitrogen PureLink Genomic DNA Mini Kit.
Genotyping has become an essential application in clinical trials for pharmacogenomics studies. The emergence of pharmacogenomic centers of excellence has resulted in an increasing need for purification of high-quality genomic DNA from large volumes of blood. Since samples are collected and shipped worldwide, every sample may require different storage and shipping conditions. Choosing the right genomic DNA extraction from blood is important based on your needs and expected end results. We offer a wide range of kits designed for DNA extraction from blood or serum samples at the purity and scale you need.
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Process the largest amount of blood | Fast isolation of gDNA from blood | High-yield, high-purity gDNA in a plate format | Rapid and automated extraction of DNA | Fastest automated extraction for blood and saliva | |
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![]() DNAzol BD Reagent | ![]() PureLink Genomic DNA Mini Kit | ![]() PureLink Pro 96 Genomic DNA Purification Kit | ![]() MagMAX-96 DNA Multi-Sample Kit | ![]() MagMAX DNA Multi-Sample Ultra Kit 2.0 | |
Blood input | 500 µL | 200 µL | 200 µL | 50–350 µL | 50 µL–2 mL |
Yield | 10–20 µg | 3–10 µg | 3–10 µg | 1.5–16 µg | 1.5–80 µg |
Isolation method | Organic extraction | Silica membrane | Filter plate | Magnetic beads | Magnetic beads |
High-throughput compatible | |||||
Compatible applications | Cloning, qPCR, sequencing | Cloning, qPCR, sequencing, genotyping | Cloning, qPCR, sequencing, genotyping | Cloning, qPCR, sequencing, genotyping | Cloning, qPCR, sequencing, genotyping |
Prep time | 10–30 mins | 15 mins | 35 mins | 90 mins using KingFisher instruments | 45 mins using KingFisher instruments |
Prep size | 100 mL | 50 and 250 preps | 4 x 96 preps | 50, 96, 500, and 2,500 preps | 100 preps or bulk reagents |
Plant DNA extraction has unique challenges that require kits specifically designed to deal with carbohydrates, phenolics, and other compounds abundant in plant tissues. Plant cell walls can be very difficult to disrupt, and lysates often contain significant amounts of compounds such as tannins, phenolics, and complex polysaccharides that can affect DNA quality and inhibit downstream reactions. We offer an expansive portfolio of kits, reagents, devices for extraction, purification, analysis, and quantitation of plant DNA. Make your DNA isolation from plant samples easier on you and easier on your samples, while achieving high-yield and high-purity results with our plant molecular biology reagents.
Most cost-effective | High quality gDNA at a great value | Fast isolation of gDNA from a variety of plant samples | Rapid and automated extraction of DNA | |
![]() Plant DNAzol Reagent | ![]() PureLink Genomic Plant DNA Purification Kit | ![]() PureLink Pro 96 Genomic DNA Purification Kit | ![]() MagMAX DNA Plant Isolation | |
Plant tissue input | 100 mg | 100 mg | up to 25 mg | up to 100 mg |
Yield | Varies based on starting material | 1–15 µg | 5–10 µg from tissue | Varies based on starting material |
Prep time | 60 mins | 40 mins | 35 mins | 40 mins |
Isolation method | Organic extraction | Silica spin column | Filter plate | Scalable, flexible format with magnetic beads |
Automatable | ||||
High-throughput compatibility | ||||
Format | 100 mL | 50 preps | 4 x 96 preps | 96 or 384 preps |
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Plant tissue is difficult enough to work with due to the high levels of polysaccharides and polyphenols present. Why compound the issue with tedious, inefficient processes? Cetyl trimethyl ammonium bromide (CTAB) methods require excessive time and handling, limiting your throughput. Silica membrane or magnetic bead–based protocols rely on guanidine and ethanol for DNA capture, but these reagents often don’t remove inhibitors inherent in plant samples that can carry over into the final product and interfere with downstream PCR applications. This leads to frequent sample processing failure, causing you to repeat the purification—if you have the time and sample to spare. We now offer you products specifically designed for easy, high-yield, high-purity DNA purification from plant samples. Inhibitors are removed for reliable downstream results the first time.
Formalin-fixed, paraffin-embedded (FFPE) tissue samples provide a valuable source of stable nucleic acids for gene expression analysis. The clinical utility of FFPE samples is substantial, where retrospective analysis of archival tissue enables the correlation of molecular findings with the response to treatment and the clinical outcome.
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Fast and easy | Low-throughput isolation of RNA and DNA from the same FFPE section | Scalable isolation of RNA and DNA from the same FFPE section | |
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![]() Ion AmpliSeq Direct FFPE DNA Kit | ![]() RecoverAll Total Nucleic Acid Isolation Kit for FFPE | ![]() MagMAX FFPE DNA/RNA Ultra Kit | |
Prep time | 30 mins | 4.5 hrs (total time for 16 samples) 2.5 hrs hands-on time | 4 hrs (total time for 96 preps) 1 hr hands-on time |
Final product extracted | DNA | total RNA, microRNA, gDNA | total RNA, microRNA, gDNA |
Isolation method | Lysis | Column purification | Magnetic bead purification |
Deparaffinization required | |||
Automation compatible (post deparaffinization) | N/A | (Scripts for KF Flex and Duo Prime) | |
Compatible FFPE sample range | single 5–10 micron section (4–100 mm2 tissue) | up to 4 x 20 micron sections (300 mm2 tissue) | up to 2 x 20 micron sections (300 mm2 tissue) |
Sample volume | ~20µL lysate containing DNA | ~50µL purified DNA ~50µL purified RNA | ~50µL purified DNA ~50µL purified RNA |
Compatible with Ion AmpliSeq DNA panels (e.g., Cancer Hotspot Panel v2) | |||
Compatible with Ion AmpliSeq RNA panels (e.g., RNA cancer and RNA fusion) |
The fixation process causes cross-linkage between nucleic acids and proteins, and covalently modifies adenine and cytidine by the addition of monomethyl groups to the bases. As a result, the molecules are rigid and susceptible to mechanical shearing. Therefore, in order to utilize FFPE tissues as a source for DNA, a reliable method is required for its extraction from the cross-linked matrix.
There are a number of factors that can impact the overall quality and yield of DNA isolated from FFPE tissues. Here are recommendations to address several key factors:
The MagMAX FFPE DNA/RNA Ultra Kits are designed for rapid, efficient, and automatable isolation of total RNA and DNA from FFPE samples using MagMAX magnetic particles. MagMAX FFPE NA kits offer faster workflows and use less toxic reagents, without sacrificing quality in the process. These kits help deliver nucleic acid yield and purity comparable to the best-in-class RecoverAll filter-based system. The MagMAX FFPE NA kits outperform products with similar protocols, and achieve RNA and DNA yields that are comparable with longer, more hazardous, and lower-throughput protocols.
The RecoverAll Total Nucleic Acid Isolation Kit procedure requires about 45 minutes of hands-on time. FFPE samples are deparaffinized using a series of xylene and ethanol washes. Next, they are subjected to a rigorous protease digestion with an incubation time tailored for recovery of either RNA or DNA. The nucleic acids are purified using a rapid glass-filter methodology that includes an on-filter nuclease treatment, and are eluted into either water or the low-salt buffer provided.
Buccal cells are one of the most common sample types used to obtain genetic information. Buccal cells are collected by swabbing the inside of a person’s cheek. The cells contained on the swab are then lysed, releasing genomic DNA that can be purified and analyzed.
As with blood samples, buccal swabs have become a standard sample type for genotyping applications in the field of pharmacogenomics. The emergence of pharmacogenomic centers of excellence has resulted in an increasing need for purification of high-quality genomic DNA from buccal swabs. We offer kits optimized for the recovery of DNA from these informative samples.
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Fast isolation of gDNA | High-yield, high-purity gDNA in a plate format | Rapid and automated extraction of DNA, recommended for PGx | Fastest automated DNA extraction for high-volume processing | |
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![]() PureLink Genomic DNA Mini Kit | ![]() PureLink Pro 96 Genomic DNA Purification Kit | ![]() MagMAX 96 DNA Multi-Sample Kit | ![]() MagMAX DNA Multi-Sample Ultra Kit 2.0 | |
No. of swabs | 1 swab | 1 swab | 1 swab | 1–2 swabs |
Yield | Up to 6 µg | Up to 6 µg | 2–12 µg | |
Isolation method | Silica membrane | Filter plate | Magnetic beads | Magnetic beads |
High-throughput compatible | ||||
Compatible applications | Cloning, qPCR, sequencing, genotyping | Cloning, qPCR, sequencing, genotyping | Cloning, qPCR, sequencing, genotyping | Cloning, qPCR, sequencing, genotyping |
Prep time | 15 min | 35 min | 1 hr | 40 min |
Prep size | 50, 250 preps | 4 x 96 preps | 50, 96, 500, and 2,500 preps | 100 preps or bulk reagents |
Bacteria is present everywhere and as research progresses, we are furthering our understanding of the important role that bacteria play in human health. The study of the microbiome is influencing our understanding of areas such as diabetes, malnutrition, obesity, and women’s health.
Not all bacterial cells are the same, and gram positive samples can be especially challenging to lyse. We have developed a variety of product formats to address all the bacterial cell types from those easy-to-lyse to the more difficult gram positive cells.
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Most cost-effective | Fast isolation of gDNA from bacterial cells | High-yield, high-purity gDNA in a plate format | Rapid and automated extraction of DNA | |
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![]() DNAzol Reagent | ![]() PureLink Microbiome DNA Purification Kit | ![]() PureLink Pro 96 Genomic DNA Purification Kit | ![]() MagMAX 96 DNA Multi-Sample Kit | |
Cell input | Up to 3 x 107 cells | Up to 2 x 109 cells | Up to 2 x 109 cells | Up to 2 x 109 cells |
Yield | Up to 70 µg | Up to 25 µg | Up to 10 µg | 15–30 µg |
Isolation method | Organic extraction | Silica membrane | Filter plate | Magnetic beads |
High-throughput compatible | ||||
Compatible applications | Cloning, qPCR, sequencing | Cloning, qPCR, sequencing, genotyping | Cloning, qPCR, sequencing, genotyping | Cloning, qPCR, sequencing, genotyping |
Prep time | 10–30 min | 45 min | 35 min | 45 min |
Prep size | 100 mL | 50 preps | 4 x 96 preps | 50, 96, 500, and 2,500 preps |
Forensics DNA samples are often highly variable in quality, making it difficult to validate a single purification protocol that will work for high-throughput screening of various sample types. ChargeSwitch technology is designed to purify samples of variable quality using a single protocol, without centrifugation, and without the introduction of high-concentration salts or organic solvents that can interfere with downstream analytical protocols.
Optimized for STR and poor or degraded samples | |
![]() ChargeSwitch Forensic DNA Purification Kit | |
Protocol time | <15 min |
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Isolation technology | ChargeSwitch chemistry |
High-throughput compatible | |
High-throughput/automation options | iPrep ChargeSwitch Forensic Kit |
Kit size | 100 preps Order Now |
The ChargeSwitch Forensic DNA Purification Kit affords high sensitivity and robustness for poor or environmentally degraded samples. This kit is fully validated for single tandem repeat (STR) profiling and is compatible with high-throughput protocols. Use the iPrep ChargeSwitch Forensic Kit when using the iPrep Purification System to process your forensic samples.
The ChargeSwitch Forensic DNA Purification Kit allows purification of genomic DNA typically in less than 15 minutes following sample preparation, using magnetic beads with a unique, ionizable, nucleic acid-binding ligand whose charge can be switched based on the pH of the surrounding medium. Purification is achieved through a simple three-step procedure in aqueous buffers and avoids the use of guanidine, ethanol, and other hazardous reagents.
The ChargeSwitch Forensic DNA Purification Kit is suitable for use in STR analysis, for mitochondrial DNA (mtDNA) extraction from hair follicles for single nucleotide polymorphism (SNP) analysis, and includes a flexible protocol to facilitate genomic DNA isolation from a variety of forensic sample types (Figure 1), such as:
- Dried blood spots on paper and clothing
- Hair follicles and hair shafts
- Cigarette butts
- Cigarette paper
- Envelopes
- Drinking vessel swabs
- Chewing gum
- Sperm head cells
- Vaginal epithelial cells
- Door handle swabs
- Strip removed cells (e.g., hats, coats, and gloves)
- "Touch DNA" (e.g., tools, mobile phones, and microscopes)
- Dyed denim
Figure 2. The ChargeSwitch Forensic DNA Purification Kit effectively purifies DNA from challenging forensic samples. 54 cigarette butts (remains) were collected, 48 samples were purified using the ChargeSwitch Forensic Kit (red bars), 6 samples were purified using a leading competitive kit (blue bars). The DNA was quantified using picogreen and fluorimetric analysis
Figure 3. Average DNA yields by sample type. The standard CST protocol for automated isolation of genomic DNA was used to purify DNA from multiple forensic samples, plus negative controls. All samples were processed in 96-well plates using a Tecan Genesis automated liquid handling robot. The single protocol was found to be highly flexible and could handle the full range of sample types tested.
Many biological drugs are produced in cells that contain unique DNA impurities or contaminants that need to be characterized for in-process and lot release testing. The success of a quality control analytical test is critically dependent on the efficiency and reproducibility of sample preparation to remove PCR interferants. A range of Applied Biosystems PrepSEQ Kits enable superior, reproducible recovery from bioproduction samples in both manual and automated formats.
Learn more about automated nucleic acid extraction and recovery from bioproduction samples.
PrepSEQ Residual DNA Sample Preparation Kit | PrepSEQ Nucleic Acid Sample Preparation Kit | PrepSEQ Express Nucleic Acid Extraction Kit | PrepSEQ 1-2-3 Nucleic Acid Extraction Kit | |
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Cat. No. | 4413686 | A50485 | 4466351 | 4452222 |
Sample type | Biopharmaceutical production samples (in-process samples, bulk drug substance) | Biopharmaceutical production samples (cell culture, raw material, bulk drug substance) | Biopharmaceutical production samples (cell culture, raw material, bulk drug substance) | Biopharmaceutical production samples (cell culture, raw material, bulk drug substance) |
Application | Residual DNA testing | Nucleic acid quantitation | Mycoplasma & Viral detection | Mycoplasma & Viral detection |
Purification target | DNA/RNA | DNA/RNA | DNA/RNA | DNA/RNA |
Isolation technology | Magnetic beads | Magnetic beads | Magnetic beads | Magnetic Beads |
Automatable | Yes | Yes | Yes | No |
Prep time | 2 hrs | 2 hrs | < 1 hr | 2 hrs |
Prep tize | 100 Prep | 100 Prep | 52 Isolations | 100 Prep |
For use with (equipment) | Pharma KingFisher Flex | Pharma KingFisher Flex | AutoMate Express | Manual |
With the complexities of biopharmaceutical manufacturing, ensuring batch-to-batch consistency and quality involves sample preparation and nucleic acid extraction for testing. By utilizing advanced magnetic bead-based technology, PrepSEQ Sample Preparation Solution provides reliable, consistent quantitative recovery of your target DNA and RNA extraction in complex matrices. Supported sample types within the biopharmaceutical industry include widely used cell lines for Chinese hamster ovary DNA (CHO DNA) often used to study genetics, mycoplasma detection and testing, and NS0 used to produce therapeutic proteins. For samples with high protein and a low DNA concentration, use the PrepSEQ residual optimized for residual gDNA extraction.
The PrepSEQ Sample Preparation Kits provide a high-throughput automated solution in combination with the Thermo Fisher Pharma KingFisher Flex Instrument and the Applied Biosystems AutoMate Express Nucleic Acid Extraction System for low hands-on extraction of DNA and RNA. Flexible protocols are available using AutoMate Express system to isolate DNA and RNA from a single organism, or a 3-in-1 surveillance panel protocol can simultaneously extract mycoplasma, mouse minute virus (MMV), and vesivirus nucleic acid from a single sample.
Our products extract high-quality DNA with excellent reproducibility for many fundamental downstream molecular biology experiments, including:
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For Research Use Only. Not for use in diagnostic procedures.