Thermo Scientific phosphoramidites with deoxyribonucleobases for synthesis of DNA oligos are built to meet the varying requirements of oligonucleotide manufacturers from the proof of concept of research and development (R&D) to the process development of therapeutic and diagnostic applications. Backed by documentation and certificates of analysis, choose from multiple quality grades of TheraPure, standard, and fast deprotect phosphoramidites that best fit your needs.

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Premium-grade and high-purity phosphoramidites are manufactured under extreme quality control and QC testing before product release to achieve critical impurity levels. Excellent for any therapeutics application with excellent batch-to-batch reproducibility.

Availability by synthesizer compatibility

Extensive QC testing to help ensure impurities and residual solvents are within the stringent requirement for oligo therapeutics.

 Bz-dABz-dCiBu-dGdT
Applied Biosystems / AKTA Synthesizer27-2030-1227-2032-1227-2034-1227-2036-12
Expedite Synthesizer27-2030-8427-2032-8427-2034-8427-2036-84
MerMade Synthesizer27-2030-1427-2032-1427-2034-1427-2036-14

Technical specifications for TheraPure DNA phosphoramidites

  Click images to enlarge

TheraPure DNA phosphoramidites

 dA(Bz) CEPdC(Bz) CEPdG(iBu) CEPdT CEP
Cat. No.27-203027-203227-203427-2036
FormulaC47H52N7O7PC46H52N5O8PC44H54N7O8PC40H49N4O8P
Molecular weight857.95 g/mol833.93 g/mol839.92 g/mol744.82 g/mol
CAS #98796-53-3102212-98-693183-15-498796-51-1
HPLC purity≥99%
31P NMR purity≥99%
Solution clarityFree from undissolved particulate at 0.2 M solution in ACN
AppearanceWhite to pale yellow powder
Residual solvent≤3.0% total % by weight
Shipping/Storage conditionAmbient / –20°C
Water content≤0.3%
Material originNon-animal source (no TSE/BSE risk)

Compatible products by synthesizer model

Compatible products are in amber bottles with serum vial neck style as ready-to-ship catalog SKU. Available in sizes of 1 g or 2 g. 

Available TheraPure DNA phosphoramidites are compatible with the following oligonucleotide synthesizers: 

  • Applied Biosystems / AKTA Synthesizer
  • Expedite Synthesizer
  • MerMade Synthesizer

Applied Biosystems and AKTA synthesizer (serum vial)

Expedite synthesizer

Available catalog SKUs are supplied in 30 mL amber bottle with a 20-400 finish neck style, which is compatible with the Expedite synthesizer for oligo synthesis.

MerMade systems synthesizer

Catalog SKUs available that are compatible with the MerMade synthesizer for oligo synthesis.

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Thermo Scientific Standard DNA phosphoramidites

Traditional base protection methods to help ensure quality and reliability of routine oligonucleotide synthesis. This quality grade supports research application (research use only, RUO) with a smooth transition to our TheraPure grade amidites, which are designed with quality attributes for therapeutics.

Availability by synthesizer compatibility

 Bz-dABz-dCiBu-dGdTdUdI
Applied Biosystems/AKTA Synthesizer27-1730-0427-1732-0427-1734-0427-1736-0427-1738-0227-1744-03
Expedite Synthesizer27-1730-1127-1732-1127-1734-1127-1736-1127-1738-08 
MerMade Synthesizer27-1730-1327-1732-1327-1734-1327-1736-13  

Technical specifications for standard DNA phosphoramidites

  Click images to enlarge

 dA(Bz) CEPdC(Bz) CEPdG(iBu) CEPdT CEP
Cat. No. 27-173027-173227-173427-1736
FormulaC47H52N7O7PC46H52N5O8PC44H54N7O8PC40H49N4O8P
Molecular weight857.95 g/mol833.93 g/mol839.92 g/mol744.82 g/mol
CAS #98796-53-3102212-98-693183-15-498796-51-1
HPLC purity≥98.0%
31P NMR purity≥98.0%
Water content≤0.3%
Shipping/Storage conditionAmbient / –20°C
Solution clarityFree from undissolved particulate at 0.2 M solution in ACN
Material originNon-animal source (no TSE/BSE risk)

Compatibility by synthesizer model

Standard DNA phosphoramidites are compatible with the following oligonucleotide synthesizers: 

  • Applied Biosystems / AKTA Synthesizer
  • Expedite Synthesizer
  • MerMade Synthesizer

Applied Biosystems and AKTA synthesizer (serum vial)

All compatible products are available as ready-to-ship catalog SKU in sizes of 1 g or 5 g. For additional size options or custom packaging services, please contact us.

Expedite synthesizer

Available catalog SKUs compatible with the Expedite synthesizer for oligo synthesis.

MerMade synthesizer

Catalog SKUs available that are compatible with the MerMade synthesizer for oligo synthesis.

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Fast deprotect DNA phosphoramidites

Fast deprotect phosphoramidites permit the removal of base protecting groups under mild or accelerated conditions without the need for special reagents. Excellent for applications requiring mild deprotection to preserve the integrity of oligonucleotides containing sensitive dyes, labels, or other modifications. 

Availability by synthesizer compatibility

 PAC-dAAc-dCDMF-dGiPrPAC-dG
Applied Biosystems/AKTA Synthesizer27-1723-0427-2027-0727-1737-0727-1726-04
Expedite Synthesizer 29-1727-1027-1737-10 
MerMade Synthesizer 29-1727-1327-1737-13 

Technical specifications for fast deprotect DNA phosphoramidites

  Click images to enlarge

 iPrPAC-dG CEPPAC-dA CEPAc-dC CEPDMF-dG CEPIBU-dC CEP
Cat. No. (TheraPure phosphoramidites)N/AN/A27-2027N/A
N/A
Cat. No. (standard phosphoramidites)27-172627-172329-172727-173727-1725
FormulaC51H60N7O9PC48H54N7O8PC41H50N5O8PC43H53N8O7PC43H54N5O8P
Molecular weight946.05 g/mol887.96 g/mol771.8 g/mol824.9 g/mol839.92g/mol
CAS #150065-82-0110543-74-3154110-40-4330628-04-1110522-84-4
HPLC purity | (TheraPure phosphoramidites)≥98.0%≥98.0%≥98% | (≥99%)≥98.0%≥98.0%
31P NMR purity | (TheraPure phosphoramidites)≥98.0%≥98.0%≥98% | (≥99%)≥98.0%≥98.0%
Shipping/Storage
condition		Ambient / –20°C
 Material origin Non-animal source (no TSE/BSE risk)

Compatibility by synthesizer model

Standard DNA phosphoramidites are compatible with the following oligonucleotide synthesizers: 

  • Applied Biosystems / AKTA Synthesizer
  • Expedite Synthesizer
  • MerMade Synthesizer

Applied Biosystems and AKTA synthesizer (serum vial)

All compatible products are available as ready-to-ship catalog SKU in size ranges of 100 μM, 100 µmol, 0.25 g, 1 g or 5 g. For additional size options or custom packaging services, please contact us.

Expedite synthesizer

Available catalog SKUs compatible with the Expedite synthesizer for oligo synthesis.

MerMade synthesizer

Catalog SKUs available that are compatible with the MerMade synthesizer for oligo synthesis.

Quality manufacturing and analytical method

Manufacturing quality

HPLC-MS, GC, 31P NMR, and Karl Fischer oven titration are used to assess the purity of each phosphoramidite lot. The rigorously controlled manufacturing processes help to minimize levels of reactive P(III) impurities and accumulation of by-products during oligo synthesis. Processes are monitored from material procurement through final QC and product release for batch-to-batch reproducibility under the ISO 9001:2015–certified quality management system.

Analytical method and data

Phosphoramidites are the key raw material and building blocks of oligonucleotide therapeutics. Incorporating the quality testing step into the manufacturing process development from the very beginning is a best practice needed for regulatory documentation. 

Example: TheraPure Bz-dA phosphoramidite

For the successful synthesis of a therapeutic from discovery to market, a thorough understanding on the analysis of phosphoramidites and impurity is crucial. Here is information on the analytical method used and the details on the instrument conditions used to test the quality of the raw material phosphoramidites.

UHPLC analysis method

Condition setup
UHPLC systemThermo Scientific Vanquish UHPLC System
DetectionThermo Scientific Vanquish Diode
Array Detector, 260 nm
ColumnPhenomenex Luna C18(2) Column,
2.0 x 150 mm, 3 µm (Cat. No. 00F-4251-B0)
Column temperature25°C
Sample temperature4°C
Injection volume2.0 µL
Mobile phase A5 mM tributylammonium formate, 30% acetonitrile
Mobile phase B5 mM tributylammonium formate, 100% acetonitrile
Sample preparation5 mg/mL in acetonitrile dried over molecular sieves
Gradient
Time (min)Flow (mL/min)A (%)B (%)
00.257822
450.251090
690.251090
700.257822
900.257822

Figure 1. Impurity profile of TheraPure Bz-dA phosphoramidite from UHPLC chromatograms

  • Full chemical name: 5'-O-(4,4'-Dimethoxytrityl)-N6-benzoyl-2'-deoxyadenosine-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
  • HPLC purity: ≥99%; M-11 Impurity ≤0.01%; Single critical impurity ≤0.3%; Presence of dC, dG, or T impurity ≤0.03%
  • 31P NMR purity: ≥99%; Sum of non-primary peaks at 140–152 ppm: ≤0.3%; No peak(s) above 170 ppm; HMT impurity (∼146 ppm): ≤0.10%

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Resources

Phosphoramidite Learning Resource

Access webinars, videos, articles, and more to optimize your success in oligonucleotide synthesis for diagnostic and therapeutic manufacturing process development.

mRNA Production Learning Library

Browse the educational assets related to raw materials selection, process development using in vitro transcription, scale-up synthesis, and the latest trends in mRNA production.

Stylesheet for Classic Wide Template adjustments

For Research Use Only. Not for use in diagnostic procedures.