TaqMan Array MicroRNA Cards are the ideal solution for profiling large numbers of miRNAs. Benefits include:

  • Results the same day—complete an experiment profiling hundreds of miRNAs in as little as 5 hours.
  • Ideal for human, mouse and rat profiling.
  • Requires only minute sample amounts—as little as 1 ng of total RNA input—making it suitable for FFPE, FACS, biopsy, and other very small samples.
  • Comprehensive coverage of known miRNAs consistent with the miRBase database.

TaqMan Advanced miRNA Assays on cards and plates

Our TaqMan Advanced miRNA chemistry on a TaqMan Array Card or OpenArray Plate. Enable highly sensitive and specific quantification of miRNAs in a high throughput format.

Search for pre-designed array plates ›
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Design your own custom array card ›
Taqman™ OpenArray Human Advanced MicroRNA Panel ›

Predesigned TaqMan Array MicroRNA Cards

Taqman array microRNA cards using classic chemistry include coverage of the most relevant microRNA for human or rodent species, aligned with Sanger miRBase v22, with over 740 assays enabled across the sets of TaqMan™ Array MicroRNA Cards (Cards A and B).

This product requires the use of Megaplex™ Primer Pools for miRNA reverse transcription. Optional Megaplex™ PreAmp Primers can be used for preamplification of limited samples prior to real-time PCR using the TaqMan Array MicroRNA Card.

TaqMan Array miRNA Cards using the Advanced chemistry enable accurate quantitation of the most common human mature miRNAs, mapped to miRBase v22. The Human A and B Cards contain 754 unique miRNAs. The Human Endogenous Controls Card contains 32 unique microRNA control targets. The Human Serum/Plasma Card contains 192 unique microRNA targets known to be present in serum/plasma.

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Custom MicroRNA Cards

 Custom miRNA array cards are available in both Classic and Advanced chemistries. They can hold from 12 to 380 assays, and 1 to 8 samples on each array card. Ten formats are offered, available only with microfluidics card design.

  • Custom RT pools are provided for each classic chemistry custom miRNA array card order.
  • Custom PreAmp pools are available for classic chemistry custom miRNA array cards to enable the study of samples with limited miRNA amount.

Configure Custom TaqMan MicroRNA Cards


TaqMan Array MicroRNA Card workflow - Classic Chemistry

Applied Biosystems offers a variety of kits for the isolation and analysis of miRNA and other small RNAs, each is ideal for use in miRNA analysis because they enable quantitative recovery of small RNA (<200 nt) and maintenance of representative amounts of small RNA (eliminating experimental bias).

Recommended Products

TaqMan® miRNA ABC Purification Kit 

TaqMan miRNA ABC Purification ™Kit
Purify high-quality miRNA from difficult sample types without hazardous chemicals. This kit enables single-tube isolation of specific miRNA from small inputs of all human sample types, including blood, serum, plasma, FFPE samples, solid tissues, cultured cells, and saliva in 75 minutes.

 

mirVana™ miRNA Isolation Kit
Isolate total RNA that contains small RNAs such as miRNA, siRNA, and snRNA. These small RNAs are often lost during traditional RNA isolation methods.

 

RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE
Extract total RNA or DNA from formalin- or paraformalin-fixed, paraffin-embedded (FFPE) tissues. This kit enables the molecular analysis of archived tissue samples at both the genomic and gene expression levels. With this kit, nucleic acid is obtained that is suitable for RT-qPCR, mutation screening, and microarray analyses.

 

TaqMan™ MicroRNA Cells-to-CT™ Kit
Featuring a unique method for lysing cultured cells while removing genomic DNA and preserving RNA integrity, the TaqMan MicroRNA Cells-to-CT™ Kit makes it possible to perform miRNA expression profiling directly from cultured cells without RNA purification.

Designed to streamline miRNA profiling, Megaplex™ RT Primers are ideal for global miRNA expression profiling; just two pools provide comprehensive coverage of the Sanger miRBase database content for human and rodent. The content of each pool matches corresponding TaqMan® Array Human or Rodent MicroRNA Cards (Step 4). The TaqMan® MicroRNA Reverse Transcription (RT) Kit provides optimal TaqMan® MicroRNA Assay performance. This kit is used with the single RT primer provided with an individual TaqMan® MicroRNA Assay, or with Megaplex™ RT Primers, to convert miRNA to cDNA prior to real-time PCR quantitation.

 
 

TaqMan® MicroRNA Reverse Transcription Kit
The TaqMan® MicroRNA Reverse Transcription Kit provides the necessary components for optimal performance of TaqMan® MicroRNA Assays. Components of this kit are used with the RT primer provided with the TaqMan® MicroRNA Assay to convert miRNA to cDNA.

Megaplex™ PreAmp Primers generate a comprehensive miRNA expression profile using as little as 1 ng of input total RNA. With content matched to specific Megaplex™ RT Primers and TaqMan® Array Human or Rodent MicroRNA Cards, cDNA prepared by reverse transcription can be preamplified using Megaplex™ PreAmp Primers and TaqMan® PreAmp Master Mix to uniformly amplify all miRNAs present in the original sample significantly increasing assay sensitivity.

 

Megaplex™ PreAmp Primers
For use when assay sensitivity is of the utmost importance, Megaplex™ PreAmp Primers significantly enhance the ability to detect low expressed miRNAs enabling the generation of a comprehensive expression profile using far less sample, as low as 1 ng of input total RNA.

 

TaqMan® PreAmp Master Mix
TaqMan® PreAmp Master Mix preamplifies small amounts of cDNA without introducing amplification bias to the sample. Preamplification enables you to stretch your precious sample into many more real-time PCR reactions.

For the final quantitation step, TaqMan® Universal Master Mix II is added to each sample and pipetted into the sample loading ports of a TaqMan® Array Card, greatly simplifying assay set-up and minimizing variability due to pipetting.

 

TaqMan® Array MicroRNA Cards
TaqMan® Array MicroRNA Cards are the ideal solution for profiling large numbers of miRNAs. This product requires the use of Megaplex™ Primer Pools for miRNA reverse transcription (Step 2).  Optional Megaplex™PreAmp Primers can be used for preamplification (Step 3) of limited samples prior to real-time PCR using the TaqMan® Array MicroRNA Card (Step 4).

 

  

 

TaqMan® Universal Master Mix II
This kit contains components needed for all 5' nuclease assays using TaqMan®-probe PCR applications. This easy-to-use solution minimizes your assay set-up time and employs AmpliTaq Gold® DNA polymerase for enhanced performance.

Step 1 : Prepare Sample

Applied Biosystems offers a variety of kits for the isolation and analysis of miRNA and other small RNAs, each is ideal for use in miRNA analysis because they enable quantitative recovery of small RNA (<200 nt) and maintenance of representative amounts of small RNA (eliminating experimental bias).

Recommended Products

TaqMan® miRNA ABC Purification Kit 

TaqMan miRNA ABC Purification ™Kit
Purify high-quality miRNA from difficult sample types without hazardous chemicals. This kit enables single-tube isolation of specific miRNA from small inputs of all human sample types, including blood, serum, plasma, FFPE samples, solid tissues, cultured cells, and saliva in 75 minutes.

 

mirVana™ miRNA Isolation Kit
Isolate total RNA that contains small RNAs such as miRNA, siRNA, and snRNA. These small RNAs are often lost during traditional RNA isolation methods.

 

RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE
Extract total RNA or DNA from formalin- or paraformalin-fixed, paraffin-embedded (FFPE) tissues. This kit enables the molecular analysis of archived tissue samples at both the genomic and gene expression levels. With this kit, nucleic acid is obtained that is suitable for RT-qPCR, mutation screening, and microarray analyses.

 

TaqMan™ MicroRNA Cells-to-CT™ Kit
Featuring a unique method for lysing cultured cells while removing genomic DNA and preserving RNA integrity, the TaqMan MicroRNA Cells-to-CT™ Kit makes it possible to perform miRNA expression profiling directly from cultured cells without RNA purification.

Step 2 : Select Megaplex™ Primer pools for Reverse Transcription

Designed to streamline miRNA profiling, Megaplex™ RT Primers are ideal for global miRNA expression profiling; just two pools provide comprehensive coverage of the Sanger miRBase database content for human and rodent. The content of each pool matches corresponding TaqMan® Array Human or Rodent MicroRNA Cards (Step 4). The TaqMan® MicroRNA Reverse Transcription (RT) Kit provides optimal TaqMan® MicroRNA Assay performance. This kit is used with the single RT primer provided with an individual TaqMan® MicroRNA Assay, or with Megaplex™ RT Primers, to convert miRNA to cDNA prior to real-time PCR quantitation.

 
 

TaqMan® MicroRNA Reverse Transcription Kit
The TaqMan® MicroRNA Reverse Transcription Kit provides the necessary components for optimal performance of TaqMan® MicroRNA Assays. Components of this kit are used with the RT primer provided with the TaqMan® MicroRNA Assay to convert miRNA to cDNA.

Step 3 : Select Megaplex™ PreAmp Primers to Amplify cDNA (optional)

Megaplex™ PreAmp Primers generate a comprehensive miRNA expression profile using as little as 1 ng of input total RNA. With content matched to specific Megaplex™ RT Primers and TaqMan® Array Human or Rodent MicroRNA Cards, cDNA prepared by reverse transcription can be preamplified using Megaplex™ PreAmp Primers and TaqMan® PreAmp Master Mix to uniformly amplify all miRNAs present in the original sample significantly increasing assay sensitivity.

 

Megaplex™ PreAmp Primers
For use when assay sensitivity is of the utmost importance, Megaplex™ PreAmp Primers significantly enhance the ability to detect low expressed miRNAs enabling the generation of a comprehensive expression profile using far less sample, as low as 1 ng of input total RNA.

 

TaqMan® PreAmp Master Mix
TaqMan® PreAmp Master Mix preamplifies small amounts of cDNA without introducing amplification bias to the sample. Preamplification enables you to stretch your precious sample into many more real-time PCR reactions.

Step 4 : Select TaqMan Array MicroRNA Card

For the final quantitation step, TaqMan® Universal Master Mix II is added to each sample and pipetted into the sample loading ports of a TaqMan® Array Card, greatly simplifying assay set-up and minimizing variability due to pipetting.

 

TaqMan® Array MicroRNA Cards
TaqMan® Array MicroRNA Cards are the ideal solution for profiling large numbers of miRNAs. This product requires the use of Megaplex™ Primer Pools for miRNA reverse transcription (Step 2).  Optional Megaplex™PreAmp Primers can be used for preamplification (Step 3) of limited samples prior to real-time PCR using the TaqMan® Array MicroRNA Card (Step 4).

 

  

 

TaqMan® Universal Master Mix II
This kit contains components needed for all 5' nuclease assays using TaqMan®-probe PCR applications. This easy-to-use solution minimizes your assay set-up time and employs AmpliTaq Gold® DNA polymerase for enhanced performance.

Step 5 : Run TaqMan Array MicroRNA Card
Step 6 : Analyze Data

Recommended Products


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