DynaGreen Magnetic Beads

Thermo Fisher Scientific DynaGreen Protein A, Protein A/G and CaptureSelect Anti-IgG-Fc (Multi-species) magnetic beads are a highly magnetic submicron bead platform with a pioneering greener design, from manufacturing to customer delivery. Building upon Dynal’s strong foundation in biomolecule capture, the team behind Dynabeads magnetic beads has applied more than 30 years of experience with the 12 principles of green chemistry to create high performing, non-microplastic magnetic beads that give reproducible results with low non-specific binding. The choice is a better option for the environment and in line with greener lab policies, without compromising on the quality of results. Leading with DynaGreen Protein A, Protein A/G, and CaptureSelect Anti-IgG-Fc magnetic beads for immunoprecipitation (IP), these are the first products in our growing sustainable magnetic bead lineup.

DynaGreen Protein A, Protein A/G, and CaptureSelect Anti-IgG-Fc magnetic beads were produced from a desire and need to move towards more sustainable research laboratories. 12 principles of green chemistry were applied at each development step, leading to a product that’s good for both your work and the environment. Here is how DynaGreen magnetic beads are contributing to more sustainable science:

We tested DynaGreen Protein A, Protein A/G, and CaptureSelect Anti-IgG-Fc magnetic beads pulldown efficiency against competitor magnetic beads. High-quality pulldown of a high-abundance target protein (CD3) is seen with DynaGreen Protein A magnetic beads compared with magnetic beads from Thermo Fisher Pierce, Promega, Sigma, and Bio-Rad (Figure 1A). DynaGreen Protein A magnetic beads outperformed all competitors in both direct and indirect IP workflows.

Western blot analysis of DynaGreen Protein A/G magnetic beads pulldown of a low-abundance target protein (CD81) shows similar or better pulldown efficiency compared with magnetic beads from Thermo Fisher Pierce, GenScript, Millipore, Sigma, and Bio-Rad (Figure 1B). DynaGreen Protein A/G magnetic beads outperformed all competitors in the indirect IP workflow. Thermo Scientific DynaGreen CaptureSelect Anti-IgG-Fc (Multi-species) beads gave the cleanest IP, while pulling down a comparable amount of target to Thermo Scientific Pierce Protein A/G magnetic beads (indirect IP workflow), Cytiva™ Sera-Mag™ SpeedBeads Protein A/G Magnetic Beads, and Bio-Rad™ SureBeads™ Protein G Magnetic Beads (direct IP workflow).

Figure 1. Comparable or better performance with DynaGreen magnetic beads. (A) DynaGreen Protein A magnetic beads compared to five other competitor beads at indirect and direct IP of a high-abundance protein target, CD3. Lane 1: Promega Magnet Protein A (Promega), lane 2: DynaGreen Protein A (Thermo Fisher), lane 3: Protein Xtra Sepharose Protein A (Cytiva – Sigma), lane 4: Pierce Protein A (Thermo Fisher), lane 5: Dynabeads Protein A (Thermo Fisher), lane 6: DynaGreen Protein A (Thermo Fisher), lane 7: Blank/no sample, lane 8: Protein A Magnetic Sepharose (Cytiva – Sigma), and lane 9: SureBeads Protein A (BioRad). (B) DynaGreen Protein A/G magnetic beads compared to ten other competitor beads at indirect and direct IP of a low-abundance protein target, CD81. Lane 1: SeraMag Speed bead protein A/G (Sigma), lane 2: CaptureSelect IgG-Fc (ms) Magnetic Agarose Beads (Thermo Fisher), lane 3: Protein Xtra Sepharose, Protein G (Cytiva – Sigma), lane 4: Pierce Protein G (Thermo Fisher), lane 5: Pierce Protein A/G Magnetic Beads (Thermo Fisher), lane 6: DynaGreen Anti IgG-Fc (Thermo Fisher), lane 7: DynaGreen Protein A/G (Thermo Fisher), lane 8: GenScript Protein A/G Mag beads (GenScript), lane 9: Dynabeads Protein G (Thermo Fisher), lane 10: DynaGreen Protein A/G (Thermo Fisher), lane 11: DynaGreen Anti IgG-Fc (Thermo Fisher), lane 12: PureProteome Protein A/G Magnetic System (Millipore), lane 13: PureProteome Protein G Magnetic System (Millipore), lane 14: Protein G Magnetic Sepharose (Cytiva – Sigma), and lane 15: SureBeads Protein G (BioRad). Equal amounts of sample material with respect to Ab and cell lysate were used for all IP protocols according to the manufacturer’s protocols.

* High binding capacity means that a smaller amount of beads are required per IP. Competitors normally use 40–100 µl.

IP is the affinity purification of proteins, protein complexes, protein-nucleic acid complexes, and other antigens (Ag) using a specific antibody that is immobilized to a solid support such as magnetic beads. DynaGreen magnetic beads are 250-micron superparamagnetic beads that enable high performance direct and indirect IP. The sub-micron bead size of DynaGreen magnetic beads increases the available target capture surface area and provides a low sedimentation rate resulting in efficient isolation of target protein in a simple bind-incubate-elute immunoprecipitation protocol that does not require pre-clearing. The magnetic separation technology used is also rapid and gentle, thereby causing minimal physical stress to your target proteins.

Additional benefits of DynaGreen Protein A, Protein A/G, and CaptureSelect Anti-IgG-Fc magnetic beads include:

• Reproducibility, high yield, and sample purity after direct and indirect IP protocols (Figure 2)
• Adaptable protocols for downstream IP assays such as western blot and mass spectrometry
• Sustainable and holistic product design with energy efficient manufacturing, recyclable packaging, and non-microplastic magnetic bead core
• ACT labeled—third-party verification of environmental impacts
• Backed by 30 years of Dynabeads quality and innovation
• Automation compatible to Thermo Scientific KingFisher systems—40 mins from the press of a button to finished sample preparation

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Figure 2. DynaGreen Protein A, Protein A/G, and CaptureSelect Anti-IgG-Fc magnetic beads for immunoprecipitation (IP). (A) Direct IP begins by 1) adding your choice of antibody to DynaGreen magnetic beads for 30 mins, 2) add sample to the antibody-bead mix for 30 mins, 3) washing of the beads to remove unbound, non-specific binding, and 4) elute target proteins from the beads with elution buffer. (B) Indirect IP begins by 1) adding your choice of antibody to your sample for 30 mins, 2) add DynaGreen magnetic beads to the sample-antibody mix for 30 mins, 3) washing of the beads to remove unbound, non-specific binding, and 4) elute target proteins from the beads with elution buffer.

After incubation of the beads-ligand-antibody complex and washing steps, elution is the last step of both direct and indirect IP. After sufficient incubation time of the antibody, magnetic beads, and sample, the Protein A, Protein A/G, and CaptureSelect Anti-IgG-Fc magnetic beads are washed and elution buffer is added to the beads to unbind the target protein from the magnetic beads, leaving you with your target protein ready for downstream analysis such as western blot or mass spectrometry (Figure 2).

Mass spectrometry is a powerful tool for identification, quantification, and characterization of proteins in complex biological samples. A standard proteomics workflow includes sample extraction, protein isolation and digestion, HPLC separation, and analysis of the resulting peptides with tandem mass spectrometry. DynaGreen Protein A, Protein A/G, and CaptureSelect Anti IgG-Fc magnetic beads have been validated for successfully isolating antibody-protein complexes for use in downstream mass spectrometry analysis. The protocol for a suggested mass spec workflow can be viewed below:

Kingfisher protocols for DynaGreen Protein A and Protein A/G magnetic beads

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ACT is a trademark of My Green Lab.
Magne is a trademark of Promega Corporation.
Sepharose is a trademark of Cytiva Bioprocess R&D AB.
SureBeads is a trademark of Bio-Rad Laboratories, Inc.
Sera-Mag is a trademark of Hyclone Laboratories, LLC.
GenScript is a trademark of Nanjing GenScript Biotech Co.
PureProteome is a trademark of Millipore Corporation.