multiple excitation and emission curves in different colors on a plot of wavelength versus relative intensity

This section features educational resources and tools to help you choose the right combination of fluorescent antibody conjugates for your immunophenotyping experiments. This includes fluorophore guides, articles, scientific posters, application notes, videos and more.

Antibody conjugates, antibody labeling, and fluorophores features

Selection guide

illustration showing an overview of fluorescence brightness, photostability and dye performance values

Fluorophore selection guide

These selection guides feature a dashboard for each fluorophore that provide the basic properties, allowing you to find the right fluorophore for your experiment.

Scientific poster

series of flow cytometry dot plots showing different cell popluations stained with multiple fluorescently labeled antibodies

T lymphocyte immunophenotyping: 14-color flow cytometry panel design using the Attune NxT Flow Cytometer and Super Bright fluorescent dyes (PDF)

This scientific poster highlights the building of a 14-color flow cytometry panel using Invitrogen eBioscience Super Bright polymer dyes which represent a suite of bright fluorophores excited by the violet laser (405 nm).


Antibody conjugates, antibody labeling, and fluorophores learning resources

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A comparative study of 25 different CD4 conjugates 
TypeTitleCategories
Application note (2017)Validated 10-color T cell panel using the Attune NxT Flow Cytometerantibodies, Attune/Attune NxT Flow Cytometer, flow cytometry, immunology, immunophenotyping, fluorescent dyes, multicolor flow cytometry
BioProbes article (2018)A comprehensive resource for state-of-the-art flow cytometry methods. Guidelines for the use of flow cytometry and cell sorting in immunological studiesantibodies and proteins, cell counting, cell cycle, cell proliferation, flow cytometer calibration, flow cytometry, flow cytometry compensation, flow cytometry sample preparation, immunology, immunophenotyping, viability
BioProbes article (2017)Harness immune checkpoints to combat tumors—Immune checkpoint antibodies for flow cytometry, IHC, and functional bioassaysantibodies, cancer, flow cytometry, immunofluorescence (IF), immunohistochemistry (IHC)
BioProbes article (2017)Bright fluorescent polymer dyes for the violet laser—Super Bright antibody conjugatesantibodies, antibody labeling, Attune NxT, flow cytometry, fluorescence, immunophenotyping, multicolor flow cytometry, Super Bright
BioProbes article (2017)T lymphocyte immunophenotyping—14-color flow cytometry panel featuring Super Bright antibody conjugatesantibodies, Attune NxT, immunology, immunophenotyping, multicolor flow cytometry, Super Bright
BioProbes article (2014)Multiplex your cell proliferation assay with GFP, RFP, and R-PE probes—Click-iT Plus EdU Proliferation Kits for imaging and flow cytometrycell proliferation, Click-iT, cell proliferation, multicolor flow cytometry
BioProbes article (2010)Optimal pairing of dyes and antibodies for flow cytometryantibodies, fluorescence, multicolor flow cytometry, particles Qdot
BioProbes article (2009)Novel solid-phase antibody labeling Alexa Fluor, antibody labeling, flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, sample preparation, Zenon
BioProbes article (2008)Using Qdot nanocrystal primary antibody conjugates in flow cytometry fluorescent dyes, multicolor flow cytometry, nanocrystals, Qdot, sample preparation, set up
BioProbes article (2007)Quantum dots as replacements for tandem dyes in flow cytometry fluorescent dyes, multicolor flow cytometry, nanocrystals, protein labeling, Qdot
BioProbes article (2007)Simultaneously visualize surface and intracellular markers antibody labeling, cell fixatives, flow cytometry, fluorescent dyes, sample preparation, Zenon
BioProbes article (2006)A guide to antibody labeling and detection Alexa Fluor, antibody labeling, flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, nanocrystals, Qdot, Zenon
BioProbes article (2006)Multicolor immunodetection using the violet laser Alexa Fluor, antibody labeling, flow cytometry, fluorescent dyes, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot
Blog postSuper Bright conjugates for flow cytometryantibodies, Attune NxT, flow cytometry, immunophenotyping, multicolor flow cytometry, Super Bright
FluorophorePacific Blue dyeantibody conjugates, flow cytometry, immunophenotyping, violet laser
FluorophorePacific Green dyeantibody conjugates, flow cytometry, immunophenotyping, violet laser
FluorophorePacific Orange dyeantibody conjugates, flow cytometry, immunophenotyping, violet laser
FluorophoreSuper Bright 436 dyeantibody conjugates, flow cytometry, immunophenotyping, tandem polymer dyes, violet laser
FluorophoreSuper Bright 600 dyeantibody conjugates, flow cytometry, immunophenotyping, tandem polymer dyes, violet laser
FluorophoreSuper Bright 645 dyeantibody conjugates, flow cytometry, immunophenotyping, tandem polymer dyes, violet laser
FluorophoreSuper Bright 702 dyeantibody conjugates, flow cytometry, immunophenotyping, tandem polymer dyes, violet laser
FluorophoreAllophycocyanin (APC)antibody conjugates, flow cytometry, immunophenotyping, phycobiliproteins, tandem dyes
FluorophoreR-phycoerythrin (R-PE)antibody conjugates, flow cytometry, immunophenotyping, phycobiliproteins, tandem dyes
FluorophoreCyan fluorescent protein (CFP)flow cytometry, fluorescent proteins
FluorophoreGreen fluorescent protein (GFP)flow cytometry, fluorescent proteins
FluorophoreRed fluorescent protein (RFP)flow cytometry, fluorescent proteins
FluorophoreFluorophore selection guideantibody conjugates, flow cytometry, immunophenotyping
Molecular Probes HandbookPhycobiliproteins—Section 6.4antibody labeling, multicolor flow cytometry, tandem conjugates
Molecular Probes HandbookUsing the Fluorescence SpectraViewer—Note 23.1flow cytometry, fluorescence, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins
Molecular Probes HandbookZenon Technology: Versatile reagents for immunolabeling—Section 7.3antibody labeling, flow cytometry, fluorescence microscopy/fluorescence imaging, secondary detection, Zenon
Scientific poster (2018)Modular, defined assembly of antibody-based constructs using click chemistry Alexa Fluor, antibody labeling, Attune/Attune NxT, CellInsight, drug discovery, drug research, high content analysis (HCA), SiteClick, spheroids
Scientific poster (2018)Prospective cytometric study evaluating the alkaline phosphatase activity as a potential functional biomarker of primitive malignant cells in acute leukemia antibodies, Attune/Attune NxT, cancer, cell function, flow cytometry, immunophenotyping, stem cell research
Scientific Poster (2017)T-lymphocyte immunophenotyping: 14-color flow cytometry panel design using the Attune NxT Flow Cytometer and Super Bright fluorescent dyes antibodies, Attune NxT, fluorescent dyes, immunophenotyping, multicolor flow cytometry, particles, Super Bright
Scientific poster (2014)Copper-safe Click-iT Plus EdU proliferation assay: Improved compatibility with simultaneous phycobiliprotein and fluorescent protein detectioncell proliferation, Click-iT, flow cytometry, immunophenotyping
Scientific poster (2013)Improved click chemistry demonstrating EdU cell proliferation with GFP-expressing cells and R-PE-based immunophenotyping Alexa Fluor, cell cycle, cell proliferation, Click-iT, flow cytometry assays, fluorescent dyes, fluorescent proteins, immunophenotyping
Scientific poster (2008)Zenon labeling technology facilitates detection of intra-cellular phosphoprotein determinants Alexa Fluor, antibodies, cell signaling, cells, flow cytometry, fluorescent dyes, protein labeling, rare cell detection, Zenon
Selection guideHuman CD & other cellular antigensflow cytometry, immunology, immunophenotyping, extracellular staining
Selection guideMouse CD & other cellular antigensflow cytometry, immunology, immunophenotyping, extracellular staining
How-to videoTutorial: Labeling a purified antibody 
Educational video on how to label your next antibody for imaging or fluorescence. Walk through the tutorial as Molecular Probes scientists demonstrate the protocol—including all the tips and tricks you'll want to know about for your next antibody labeling experiment. The video features Judie showing Curtis, a chemistry graduate student, how to label his monoclonal antibodies while saving time and maximizing yield.
antibodies, antibody labeling, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes
How-to videoHow to site specifically label your antibody using SiteClick technology 
This video explains how to easily and site specifically label an antibody using an enzymatic and Click chemistry approach. This method can be applied to any intact IgG antibody and requires no antibody engineering or complex methodology. Unlike classic antibody conjugation techniques this breakthrough SiteClick technology allows for antibody conjugation with complete confidence that the label will not directly interfere with the antibody binding domain.
antibodies, antibody labeling, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, Qdot, western detection
WebinarA practical approach to antibody labeling 
The growing number of fluorophores available makes labeling your own antibodies a tempting proposition. But with many antibody labeling solutions available, selecting the best option can be a daunting task. In this webinar we will:
  • Provide an overview of our antibody labeling kits
  • Offer guidance on which methods are ideal for specific applications and experiments
  • Provide tips and tricks to optimize your labeling protocol
antibodies, antibody labeling, flow cytometry, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF)
WebinarLearn to choose the right fluorophore when designing experiments 
The choice of fluorophore is one of the first important decisions to make in developing an experiment. Fluorophores are compounds that emit light at a specific wavelength when they have been excited at another, lower wavelength. Join our webinar and explore:
  • How to choose the best organic dye for an assay
  • Quantum dots and how they compare to other dyes
  • When to use a phycobiliprotein like R-PE or APC
  • When to use fluorescent proteins like GFP
  • How to choose a suitable dye to match your instrument
In addition, we will explore the basic characteristics, strengths, and weaknesses of the various fluorophores to help you choose and develop the best assay for your needs.
antibodies, antibody labeling, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.