photograph of test tube tops

Sample preparation plays a critical role in producing quality data in your flow cytometry experiment. This section offers educational resources for sample preparation for intracellular staining, extracellular staining and more.

Sample preparation features

Selection guide

3D image of cell in circulation

Intracellular staining guide (for mouse & human cytokines and non-cytokine proteins)

This selection guide features buffer conditions, blocking conditions and more for select mouse and human protein antigens.

Biology overview

dot plot of flow cytometry data showing foxp3 positive cell populations compared to CD4 and CD25 populations

Foxp3: The master regulator of T regulatory cells

This biology overview of Foxp3 includes the challenges in sample preparation when studying this system.


Sample preparation learning resources

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TypeTitleCategories
BioProbes articles (Issues 50–present day)BioProbes Journal of Cell Biology Applicationcell analysis, flow cytometry, imaging microscopy, immunoassays, antibodies, protein detection and quantification
Immunology informationFoxp3 overviewflow cytometry, Foxp3, intracellular staining
ProtocolsCell preparation for flow cytometry protocols (Invitrogen eBioscience reagents)flow cytometry, sample preparation
ProtocolsRed blood cell lysis protocols using eBioscience lysis buffers (Invitrogen eBioscience reagents)flow cytometry, sample preparation
ProtocolsStaining cell surface targets for flow cytometry (Invitrogen eBioscience reagents)extracellular staining, flow cytometry, immunophenotyping
ProtocolsStaining intracellular antigens for flow cytometry (Invitrogen eBioscience reagents)flow cytometry, immunophenotyping, intracellular staining
ProtocolsSuper Bright staining buffer protocol  antibody conjugates, flow cytometry, immunophenotyping
Scientific poster (2009)A novel xeno-free and serum-free culture medium for the expansion of human mesenchymal stem cells  cell culture, cell expansion, flow cytometry, fluorescent dyes, gene expression, immunophenotyping, stem cell research
Scientific poster (2009)A simple, fast and quantitative single-step dead-cell indicator for flow cytometry  apoptosis, fluorescent dyes, multicolor flow cytometry, nucleic acid quantitation, viability
Scientific poster (2009)Dead cell stains in flow cytometry: A comprehensive analysis  apoptosis, cells, flow cytometry, fluorescent dyes, immunophenotyping, viability
Selection guideAntibody fixation considerationssample preparation
Selection guideHuman antibody cross reactivity chartantibody cross-reactivity, flow cytometry
Selection guideMouse antibody cross reactivity chartantibody cross-reactivity, flow cytometry
Selection guideCytokine bioassays guidecytokines, flow cytometry, immunophenotyping, intracellular staining
Selection guideIntracellular staining guide (for mouse & human cytokines and non-cytokine proteins)cytokines, flow cytometry, intracellular staining
Selection guideIntracellular staining buffer selection guideflow cytometry, immunophenotyping, intracellular staining
Selection guidePhospho flow cytometry antibody clone buffer selection guideflow cytometry, immunophenotyping, intracellular staining, phosphoflow cytometry

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.