3D image of cells in suspension

This section provides different examples of immunophenotyping data that can be found in application notes, articles, scientific posters, on demand webinars and more.

Immunophenotyping examples features

Application note

Validated 10-color T cell panel using the Attune NxT flow cytometer

In this application note, we take advantage of the OMIP-009 publication, which is optimized for characterizing the immunological response of human T cells, applying it to the 4-laser, 16-parameter configuration of the Invitrogen Attune NxT Flow Cytometer with Autosampler. Additionally, we discuss the importance of antibody titration and optimizing antibody concentration in panel testing. Furthermore, we review the importance of fluorescence minus one (FMO) controls.

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TypeTitleCategories
Application note (2017)Validated 10-color T cell panel using the Attune NxT Flow Cytometerantibodies, Attune/Attune NxT Flow Cytometer, flow cytometry, immunology, immunophenotyping, fluorescent dyes, multicolor flow cytometry
Application note (2017) Detection of platelets in whole blood using the Attune NxT Flow Cytometer Attune NxT, flow cytometer, platelets, immunophenotyping, no-wash, no-lyse
Application note (2015)Attune NxT Flow Cytometer for 6-color immunophenotyping analysis of stained human whole blood using a no-lyse, no-wash protocol, with no compensationAttune NxT, flow cytometer, immunophenotyping, multicolor flow cytometry
Application note (2015)Detection of murine regulatory T cells on the Attune NxT Flow CytometerAttune NxT, flow cytometer, immunophenotyping, multicolor flow cytometry
Application note (2015)Multiparameter analysis of murine regulatory T cells and dendritic cells with the Attune NxT Flow CytometerAttune NxT, flow cytometer, immunophenotyping, multicolor flow cytometry
Application note (2015)Ten-parameter immunophenotyping of human lysed whole blood with the Attune NxT Flow Cytometer allows identification of multiple T cell subsets and myeloid cellsAttune NxT, flow cytometer, immunophenotyping, multicolor flow cytometry
Application note (2015) 13-parameter immunophenotyping of human lysed whole blood with the Attune NxT Flow Cytometer allows for the identification of B cells, NK cells, multiple T cell subsets and myeloid cells Alexa Fluor, antibodies, Attune NxT, compensation, flow cytometer, fluorescent dyes, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot
BioProbes article (2018)Immuno-oncology: Advances in Basic Research and Translational Medicine. With a focus on immune checkpoint inhibitors and T cell immunotherapyantibodies, ELISA, flow cytometry, fluorescence microscopy/fluorescence imaging, high content analysis (HCA), immunohistochemistry (IHC), immunology, immuno-oncology, immunophenotyping, Luminex, ProCartaPlex, spheroids
BioProbes article (2016)Transcription factor expression during differentiation of hPSC-derived cardiomyocytes—A multiparametric approach using the Attune NxT Flow CytometerAttune NxT, flow cytometer, fluorescence, large cell analysis, rare event detection, stem cell research
BioProbes article (2016)Optimized multicolor immunofluorescence panels (OMIPs)—Jump-start your experimental design with published antibody and reagent panelsflow cytometry, fluorescence, immunophenotyping, multicolor flow cytometry
BioProbes article (2015)No-compensation immunophenotyping using a four-laser flow cytometerAttune NxT, flow cytometer, fluorescence, immunophenotyping, multicolor flow cytometry
Guided learningT cell stimulation and proliferation eLearning courseantibodies, cell proliferation, cell stimulation, flow cytometry, immunoassays, immunology, immunophenotyping, T cells
Scientific Poster (2015) A no lyse, no-wash approach to characterizing phagocyte phenotype and function in whole human blood on the Attune NxT Flow Cytometer Attune NxT, cell cycle, flow cytometer, flow cytometry assays, immunophenotyping, no lyse, phagocytosis, whole blood
Scientific poster (2010) Proliferative and phenotypic characterization of human mesenchymal stem cells by flow cytometry and imagingAlexa Fluor, cell proliferation, cells, Click-iT, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, immunofluorescence (IF), immunophenotyping, stem cell research, violet laser-excited reagents
Scientific poster (2009) Proliferative and phenotypic characterization of human mesenchymal stem cells by flow cytometry Alexa Fluor, cell proliferation, cells, Click-iT, flow cytometry, fluorescent dyes, fluorescent proteins, immunophenotyping, nucleic acid quantitation, stem cell research, violet laser-excited reagents
CYToMORE lecture video (2016) Assessing immunological aging using flow cytometry
Presented by Victor Appay, Faculté de Médecine Pierre et Marie Curie, Pitié-Salpêtrière, France
flow cytometry, immunology, immunophenotyping
CYToMORE lecture video (2016) Cytometric analysis of cytokines and transcription factors 
Presented by Hyun-Dong Chang, Deutsches Rheuma-Forschungszentrum, Berlin, Germany
cell signaling, flow cytometry, immunophenotyping
CYToMORE lecture video (2016) Heterogeneity of tumour-infiltrating leukocytes: implications for adoptive cell transfer immunotherapy 
Presented by Enrico Lugli, Humanitas Clinical and Research Center, Italy
flow cytometry, immunology, immunophenotyping
CYToMORE lecture video (2016) Mass cytometry in the phenomics era: an essential tool for gene/function studies 
Presented by Herve Luche, Centre d’Immunophénomique - CIPHE, France
mass cytometry, immunology, immunophenotyping