Beta Tubulin Antibodies

Immunofluorescent analysis of beta-3 Tubulin (green) and PAX6 (red) in human iPSC-derived forebrain organoids derived at Day 40. The organoids were fixed with 4% PFA for 1 hour at room temperature, followed by incubation with 30% sucrose solution overnight at 4°C. The organoids were then embedded in OCT and cryosectioned at 5 µm, permeabilized with 0.2% Triton X-100 for 20 minutes, and blocked with 10% donkey serum in PBS for 30 minutes at room temperature. Organoid slices were stained with a beta-3 Tubulin Monoclonal Antibody (2G10) (green; Cat. No. MA1-118) at a dilution of 1:500, and a Rabbit PAX6 polyclonal antibody (red; Cat. No. 42-6600) at a dilution of 1:500 in blocking buffer overnight at 4°C, and then incubated with Donkey anti-Mouse Alexa Fluor 488 (Cat. No. R37114) and Donkey anti-Rabbit Alexa Fluor 568 (Cat. No. A10042) at a dilution of 1:1000 as well as DAPI (blue; 1:25000) in blocking solution at room temperature for 1 hour. Images were taken at 20X magnification. Scale bar: 50 µm.

Immunohistochemistry analysis of formalin-fixed paraffin-embedded human kidney tubules stained using a beta Tubulin polyclonal antibody (Cat. No. PA1-41331) at 10 µg/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes.

Flow cytometry analysis of Beta Tubulin in CEM cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10⁶ cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a beta Tubulin Loading Control Monoclonal Antibody (BT&R) (Cat. No. MA5-16308) at a dilution of 1 µg/test for 40 minutes at room temperature. Cells were then incubated for 40 minutes at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.